Processing of East African Highland Green Bananas: Waste Generation and Characterization as a Potential Feedstock for Biogas Production in Uganda


  • Robert Gumisiriza School of Biosciences, Makerere University P.O Box 7062 Kampala, Uganda
  • Joseph Funa Hawumba School of Biosciences, Makerere University P.O Box 7062 Kampala, Uganda
  • Apollo Simon Peter Balyeidhusa School of Biosciences, Makerere University P.O Box 7062 Kampala, Uganda
  • Mackay Okure School of Engineering, Makerere University. P.O Box 7062 Kampala, Uganda
  • Oliver Hensel Universität Kassel - FG Agrartechnik, Nordbahnhofstr.1a, 37213 Witzenhausen, Germany


Banana processing, Banana waste characterization, Anaerobic Digestion, Biogas production.


Uganda is the second largest global producer of bananas and the industry generates different waste residues both at production and processing levels. This study aimed at assessing the state of banana processing, waste generation and its characterization for evaluation as feedstock for biogas production. The study was undertaken through a reconnaissance visit to western Uganda, one of the most banana producing regions. The information was collected following standard qualitative methods and laboratory analysis. Results revealed that processing of banana fruits mainly involved manual peeling of fruits to generate fresh pulp and large quantities of banana waste. The waste contained more than 80 % moisture content and volatile solids. It also had higher carbon content than total nitrogen that translated into a high C:N ratio of 41:1. The lignocellulose content comprised cellulose 21.16 %, hemicelluloses 10.46 % and lignin 11.31 %. The Biochemical Methane Potential (BMP) test showed a methane yield of 0.436 m3 CH4/KgVS which was higher than 0.340 m3 CH4/KgVS for grass. The highest methane production of 79.9 ml CH4/gVS/day was recorded at a retention time of 24 days. These results showed that banana waste was a favorable feedstock for biogas production through anaerobic digestion. Appropriate pre-treatment of lignocellulose would be required to enhance feedstock digestibility to improve biogas yield. The study concluded that utilization of banana waste via anaerobic digestion to produce biogas was the most economically viable option to alleviate the industry’s energy scarcity.


. Abdullah, N., Sulaiman, F., Miskam, M.A and Taib, R. M (2014),” Characterization of Banana (Musa spp.) Pseudo-Stem and Fruit-Bunch-Stem as a Potential Renewable Energy Resource”, International Journal of Biological, Veterinary, Agricultural and Food Engineering Vol: 8 No: 8, 2014.

. Adebayo, E.A and Martínez-Carrera, D (2015) “Oyster mushrooms (Pleurotus) are useful for utilizing lignocellulosic biomass” African Journal of Biotechnology Vol. 14 (1): 52-67.

. Allen, S.E.(1989) “Chemical analysis of ecological material. (2nd ed). Blackwell Scientific Publications; Oxford, UK.

. AOAC (2002) Association of Official Analytical Chemist. Official Methods of Analysis (17th ed.). Washington, DC.

. APHA (1998). Standard methods for examination of water and wastewater. 20 Edn. American Public Health Association, Washington, D.C.

. Asha, N., Enoch,K., Kephas, N., Susan, A. and Diego, N. (2015) “Technical report: Structure of the Cooking Banana Value Chain in Uganda and Opportunities for Value Addition and Postharvest Losses Reduction” Expanding Utilization of Roots, Tubers and Bananas and Reducing Their Postharvest Losses(RTB-ENDURE) is a 3 year project (2014-2016) implemented by the CGIAR Research Program on Roots, Tubers and Bananas (RTB) with funding by the European Union and technical support of IFAD.

. Ayeni, A. O., Hymore, F. K, Mudliar, S. N., Deskmukh, S. C., Satpute, D. B., Omoleye J. A, and Pandey, R. A (2013) Hydrogen peroxide and lime based oxidative pretreatment of wood waste to enhance enzymatic hydrolysis for a biorefinery: process parameters optimization using response surface methodology, Fuel, 106, 187-194.

. Ayeni, A.O., Adeeyo, O.A., Oresegun, O.M and Oladimeji, T.E (2015) “Compositional analysis of lignocellulosic materials: Evaluation of an economically viable method suitable for woody and non-woody biomass” American Journal of Engineering Research Vol.4, (4) pp-14-19

. Bagambe F, Kikulwe E, Tushemereirwe W K, Ngambeki D, Muhangi J, Kagezi GH, Rugama PE, Eden-Green S (2006). Awareness of Banana Bacterial W ilt Control in Uganda: 1. Farmers’ Perspective. Afr. Crop Sci. J. 14(2): 157-164

. Bardiya, N., Somayaji, D and Khanna, S (1996) “Biomethanation of banana peel and pineapple waste” Bioresource Technology 58 :73-76

. Bilibio, C., Hensel, O. and Selbach, J (2011), “Sustainable water management in the tropics and subtropics (and case studies in Brazil)”, Vol. 2 Jaguarão/RS, Fundação Universidade Ederal Do Pampa; UNIKASSEL - Pgcult-UFMA 2011

. Bouallagui H, Cheikh RB, Marouani L, Hamdi M (2003). Mesophilic biogas production from fruit and vegetable waste in a tubular digester. Bioresource Technology. 86: 85-85.

. Carpita, N. & Kanabus, J (1987) Extraction of starch by dimethylsulfoxide and quantization by enzymatic assay, Analytical Biochemistry 161, 132–139

. Chandra R., Takeuchi H., Hasegawa T. (2012): Methane production from lignocellulosic agricultural crop wastes: A review in context to second generation of biofuel production. Renewable and Sustainable Energy Reviews 16(3): 1462-1476.

. Clarke, W.P., Radnidge, P., Lai, E.T., Jensen, P and Hardin, M.T (2008) “Waste Management 28 (3), 527-533.

. Colin X, Farinet JL, Rojas O, Alazard D (2007). Anaerobic treatment of cassava starch extraction waste water using a horizontal flow filter with bamboo as support. Bioresource Technology. 98: 1602-1607.

. Dubois, M., Gilles,K.A., Hamilton, J.K., Rebers, P.A and Smith, F (1956) “Colorimetric methods for determination of sugars and related substances. Analytical Chemistry 28: 350-356.

. Emaga. T.H., Andrianaivo, R.H, Wathelet, B., Tchango, J.T and Paquot, M (2007) “Effects of the stage of maturation and varieties on the chemical composition of banana and plantain peels” Food Chemistry .103 590-600.

. Erguder, J.F., Tezel, U., Guven, E., Demirer, G.N (2001), “Anaerobic biotransformation and methane generation potential of cheese whey in batch and UASB reactors”, Waste management 21, 643-650

. Essien, J.P., Akpan, E.J and Essien, E.P (2005) “Studies on mould growth and biomass production using waste banana peel” Bioresource Technology 96: 1451–1456

. Fajardo, D., Jayanty, S.S and Jansky, S.H (2013). Rapid High Throughput Amylose Determination in Freeze Dried Potato Tuber Samples, Journal of Visual Experiments, (80): 50407

. FAOSTAT (2012): Global banana production by year, Food and Agriculture Organization of the United Nations. Retrieved on 10th May 2017 from http://faostat.fao. Org

. Graefe, S., Dufour, D., Giraldo, A., Muñoz, L. A., Mora, P., Solís, H., Garcés, H and Gonzalez, A (2011), “Energy and carbon footprints of ethanol production using banana and cooking banana discard: A case study from Costa Rica and Ecuador,” Biomass and Bioenergy, vol. 35, no. 7, pp. 2640-2649.

. Gumisiriza, R., Hawumba, J.F., Okure, M and Hensel, O (2017) “Biomass Waste-to-Energy Valorisation Technologies: A Review Case for Banana Processing in Uganda” Biotechnology for biofuels 10:11. DOI 10.1186/s13068-016-0689-5

. Gumisiriza, R., Mshandete, A.M., Rubindamayugi, M.S.T., Kansiime, F. and Kivaisi, A. K (2009), “Enhancement of Anaerobic digestion of Nileperch fish processing waste water”, African Journal of Biotechnology 8: (2) 328-333

. Hovenkamp-Hermelink J, Devries J, Adamse P, Jacobsen E, Witholt B, Feenstra W. (1988) Rapid estimation of the amylose amylopectin ratio in small amounts of tuber and leaf tissue of the potato. Potato Research; 31:241–246.

. Jeon, Y.J., Xun, Z. and Rogers, P.L (2010). “Comparative evaluations of cellulosic raw materials for second generation bioethanol production” Letters of Applied Microbiology. 51: 518-24.

. Kirtane, R. D., Suryawanshi, P.C., Patil, M.R., Chaudhari, A.B and Kothari, R.M (2009), “Optimization of Organic Loading Rate for different fruit waste during biomethanization”, Journal of Scientific & Industrial Research Vol.68, pp252-255

. Kiyasudeen,S.K., Hakimi bin Ibrahim, M and Ismail, S. A (2015) “Characterization of Fresh Cattle Wastes Using Proximate, Microbial and Spectroscopic Principles” American-Eurasian Journal of Agricultural & Environmental Science, 15 (8): 1700-1709.

. Lyimo, T.J, Pol, A and Op den Camp, H.J.M (2002a) “Methane emission, sulphide concentration and redox potential profiles in Mtoni mangrove sediment, Tanzania”. Western Indian Ocean Journal of Marine Science 1: 71–80.

. Martin-Ryals, (2012), “Evaluating the potential for improving anaerobic digestion of cellulosic waste via routine bioaugmentation and alkaline pretreatment”, A thesis Submitted in partial fulfillment of the requirements for the degree of Master of Science in Agricultural and Biological Engineering in the Graduate College of the University of Illinois at Urbana-Champaign, 2012

. Mohammad J. Taherzadeh, M.J and Karimi, K (2008) “Pretreatment of Lignocellulosic Wastes to Improve Ethanol and Biogas Production: A Review” International Journal of Molecular Sciences 9, 1621-1651; DOI: 10.3390/ijms9091621

. Monsalve, J.F.G., De perez, V.I.M. and Colorado, A.A.R (2006) “Ethanol production of banana shell and cassava starch” Dyna, Año 73, Nro. 150, pp. 21-27

. Moody, L., Burns, R., Wu-haan, and Spajic, R (2009), “Use of Biochemical Methane Potential (BMP) Assays for Predicting and Enhancing Anaerobic Digester Performance”, Agricultural and Biosystemes Engineering, Lowa State University, Digital Repository: Conference Proceedings and Presentations.

. Mshandete, A., Björnsson, L., Kivaisi, A.K, Rubindamayugi, S.T. and Mattiasson, B (2005), “Enhancement of anaerobic batch digestion of sisal pulp waste by mesophilic aerobic pre-treatment”, Water Research 39, 1569-1575.

. Mshandete, A., Björnsson, L., Kivaisi, A.K, Rubindamayugi, S.T. and Mattiasson, B (2005), “Enhancement of anaerobic batch digestion of sisal pulp waste by mesophilic aerobic pre-treatment”, Water Research 39, 1569-1575

. Newenhouse SC, Schmit JT (2000). Qualitative methods add value to waste characterization studies. Waste Management Research.18: 105-114.

. Padam, B.S.,Tin,H.S., Chye,F.Y and Abdullah,M.I (2014), “ Banana by-products: an under-utilized renewable food biomass with great potential”, Journal of Food Science and Technology 51 (12), 3527-3545.

. Patrick, F., Mtui, G., Mshandete, A.M and Amelia Kivaisi, A (2011). Optimization of laccase and manganese peroxidase production in submerged culture of Pleurotus sajor- caju, African Journal of Biotechnology Vol. 10 (50), 10166-10177

. Prabhudessai, V.,Ganguly, A, and Mutnuri, S (2013), “Biochemical Methane Potential of Agro Wastes”, Journal of Energy. Vol: 7

. Salyeem, E .J., Mshandete, A.M and Kivaisi, A.K (2014) “Biovalorization of Banana waste: Auditing and improvement of bio-methane production by fungal pre-treatment” Journal of Chemical, Biological and Physical Sciences, Vol. 4, No. 1; 818-833.

. Singh, S., Kumar, S., Jain, M.C and Kumar, D, (2001) “Increased biogas production using microbial stimulants” Bioresouce Technology Vol. 78 (3): 313-316.

. Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J and Templeton, D (2008) Determination of structural carbohydrates and lignin in biomass: laboratory analytical procedure (LAP). Golden, CO: National Renewable Energy Laboratory; NREL Report No.: TP-510-42618. Contract No.: DE-AC36-99-G010337, Sponsored by the U.S. Department of Energy.

. Smith, A. M and Zeeman, C.S (2006), Quantification of starch in plant tissues. Protocol; 2006 Nature Publishing Group;

. Spilsbury, J.S., Jagwe, J.N., Ferris, R.S.B (2002), “Evaluating the Marketing Opportunities for Banana & its Products in the Principle Banana Growing Countries of ASARECA (Uganda)”, International Institute of Tropical Agriculture. Foodnet. P: 60.

. Steffen, R., Szolar, O. and Braun, R (1998) “Feedstocks for Anaerobic Digestion” University of Agricultural Sciences Institute for Agrobiotechnology. Tulln, Vienna. Q:RODLPROJEKTEAD-NETTFEEDNEW.DOC

. Tock, J.Y., Lai, C.L., Lee, K.T., Tan, K.T and Bhatia, S (2010), “Banana biomass as potential renewable energy resource: A Malaysian case study,” Renewable & Sustainable Energy Reviews, vol. 14, no. 2, pp 798-805Moody, L.B. and Roman, D.R (2001), “A dual-anaerobic system for complete treatment of food processing waste”, Journal of Agricultural Engineering Research 80, 293-299

. Tripathi, L., Tripathi1, J. N. and Tushemereirwe, W. K (2008), “Rapid and efficient production of transgenic East African Highland Banana (Musa spp.) using intercalary meristematic tissues”, African Journal of Biotechnology Vol. 7 (10), pp. 1438-1445

. Tumutegyereize, P., Muranga, F.I., Kawongolo, J. and Nabugoomu, F (2011), “Optimization of biogas production from banana peels”, African Journal of Biotechnology Vol. 10 (79), 18243-18251

. Undersander, D., Mertens, D. R and Thiex, N (1993) “Forage Analysis procedures; National Forage Testing Association, Omaha, USA

. Velásquez-Arredondo, H.I., Ruiz-Colorado, A.A and De Oliveira junior, S (2010) “Ethanol production process from banana fruit and its lignocellulosic residues” Energy analysis 35: 3081-3087

. Waiswa, A.N.A (1998), Anaerobic treatment of distillery slope and landfill leachate using UASB and hybrid reactors, Master of Science thesis, University of Dar es salaam.

. Yan Q, Hua C, Yang S, Li Y, Jiang Z. (2012) “High level expression of extracellular secretion of a β-glucosidase gene (PtBglu3) from Paecilomyces thermophila in Pichia pastoris”. Protein Expression and Purification. 2012;84:64–72. doi: 10.1016/j.pep.2012.04.016.

. Zaher, U., Grau, P., Benedetti, L., Ayesa, E., Vanrolleghem, P.A (2007). Transformers for interfacing anaerobic digestion models to pre- and post-treatment processes in a plant-wide modelling context. Environmental Modelling and Software, 22, 40-58.




How to Cite

Gumisiriza, R., Funa Hawumba, J., Peter Balyeidhusa, A. S., Okure, M., & Hensel, O. (2019). Processing of East African Highland Green Bananas: Waste Generation and Characterization as a Potential Feedstock for Biogas Production in Uganda. American Scientific Research Journal for Engineering, Technology, and Sciences, 53(1), 215–236. Retrieved from