Antimicrobial Activity of Lemon (Citrus limon) Peel Extract Against Escherichia coli


  • Alhoi Hendry Henderson Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia
  • Edy Fachrial Laboratory of Molecular Biology, Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia
  • I. Nyoman Ehrich Lister Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia


Citrus limon peel, Escherichia coli, antimicrobial.


In developing country herbal medicine used widely to treat infectious disease. The Citrus limon peel rich in nutrient such as flavonoid and essential oil that can used for antimicrobial and anticancer activity. The Citrus limon peel extracted with ethanol 96% using maceration extraction and then dissolved with DMSO. The antimicrobial activity tested against Escherichia coli using disc diffusion assay. The present study shows the antimicrobial activity of the ethanolic extract of the Citrus limon peel of 25%, 50%, 75% and 100% had a strong antimicrobial activity against Escherichia coli were 15,03 mm; 16,17 mm; 15,83 mm; 18,77mm of the average inhibition zone, respectively. The essential oil can disrupt the bacterial membrane and damage the lipids and proteins layer. The flavonoid can inhibit specific enzymes and scavenge free radical. It concluded that lemon (Citrus limon) peel extract have a high potential on antimicrobial activity against the Escherichia coli.


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How to Cite

Henderson, A. H., Fachrial, E., & Lister, I. N. E. (2018). Antimicrobial Activity of Lemon (Citrus limon) Peel Extract Against Escherichia coli. American Scientific Research Journal for Engineering, Technology, and Sciences, 39(1), 268–273. Retrieved from