Molecular Study of Urinary Tract Infection Bacteria and their Relationship to the Present of Oxalobacter formigenes in Stool of Kidney Stone Patients

  • Munaff J. Abd Al-Abbas Department of Biology, College of Science, University of Basrah, Basrah, Iraq
  • Nooraldeen A. Jasim Department of Biology, College of Science, University of Basrah, Basrah, Iraq
Keywords: Molecular, 16SrDNA, Sequencing, Oxalobacter, Kidney, Corynebacterium, aurimucosum.

Abstract

Urinary Tract Infection is widespread in Al-Basrah province, it is important to detect the causative agent for suitable treatment and which factors may affect on the disease. Fifty urine samples were collected from patients with urinary  stone disease from Al-Basrah General Hospital. Bacterial growth was in 48 samples. Axenic culture appeared 82%  compared with mixed or no growth. Out  of all bacterial isolates (55), gram – positive was  71% versus to gram – negative. 16SrDNA gene sequencing identified 10 Staphylococcus haemolyticus , 7 Escherechia coli, 5 Staphylococcus epidermidis and 3 Enterococcus faecalis. While Pseudomonas  aeruginosa, Klebsiella pneumonia, Streptococcus agalactiae, Staphylococcus hominis and Bacillus cereus were 2 for each. Furthermore, Bacillus subtilis, Enterococcus raffinosus, Staphylococcus lugdunesis, Staphylococcus warneri, Streptococcus parasanguinis, Corynebacterium amycolatum, Corynebacterium tuberculostearicum, Corynebacterium coyleae and  Corynebacterium aurimucosum were 1 for each. Four of these isolates were recorded as new global strains in GenBank. The bacterial species were more frequented in  patients without surgical operation (88.6%), antibiotic utilization (77.3%), kidney and oxalate stone (72.7%) for each. Most  bacterial isolates had a high sensitivity to imipenem (86.4%), then followed by amikacin and ciprofloxacin (54.5%, for each), but  low and no sensitivity to ceftriaxone (97.7%), ampiclox and methicillin (100%, for each). High rate of  multi - drug resistance was noticed among all isolates. This is a first study confirms a relationship between the presence of some UTI bacteria and the absence of Oxalobacter  formigenes in the gut.

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Published
2016-10-14
Section
Articles