Molecular Study of Urinary Tract Infection Bacteria and their Relationship to the Present of Oxalobacter formigenes in Stool of Kidney Stone Patients
Keywords:Molecular, 16SrDNA, Sequencing, Oxalobacter, Kidney, Corynebacterium, aurimucosum.
Urinary Tract Infection is widespread in Al-Basrah province, it is important to detect the causative agent for suitable treatment and which factors may affect on the disease. Fifty urine samples were collected from patients with urinary stone disease from Al-Basrah General Hospital. Bacterial growth was in 48 samples. Axenic culture appeared 82% compared with mixed or no growth. Out of all bacterial isolates (55), gram – positive was 71% versus to gram – negative. 16SrDNA gene sequencing identified 10 Staphylococcus haemolyticus , 7 Escherechia coli, 5 Staphylococcus epidermidis and 3 Enterococcus faecalis. While Pseudomonas aeruginosa, Klebsiella pneumonia, Streptococcus agalactiae, Staphylococcus hominis and Bacillus cereus were 2 for each. Furthermore, Bacillus subtilis, Enterococcus raffinosus, Staphylococcus lugdunesis, Staphylococcus warneri, Streptococcus parasanguinis, Corynebacterium amycolatum, Corynebacterium tuberculostearicum, Corynebacterium coyleae and Corynebacterium aurimucosum were 1 for each. Four of these isolates were recorded as new global strains in GenBank. The bacterial species were more frequented in patients without surgical operation (88.6%), antibiotic utilization (77.3%), kidney and oxalate stone (72.7%) for each. Most bacterial isolates had a high sensitivity to imipenem (86.4%), then followed by amikacin and ciprofloxacin (54.5%, for each), but low and no sensitivity to ceftriaxone (97.7%), ampiclox and methicillin (100%, for each). High rate of multi - drug resistance was noticed among all isolates. This is a first study confirms a relationship between the presence of some UTI bacteria and the absence of Oxalobacter formigenes in the gut.
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