Comparative Genomics of Enterococcus Faecium Bacteriophages
Keywords:E. faecium, Prophages, Enterococcus, Comparative Genomics.
Temperate bacteriophages are known to be important drivers of genome plasticity in E. faecium species. The diversity of prophages and their relationship between was investigated after locating 56 prophage elements containing integrase and lysin genes encoded in the 139 publicly available E. faecium genomes by the end of 2014. Comparative analysis of the seprophages identified eight sequence types, which differed in size and gene content. The prophage genomes comprised between 17 to 72 ORFs and their size ranged from 13.9 to 55.1 kb with 35% to 37.9% average G+C content. Based on alignment analyses of the major functional proteins encoded in the prophage genomes (integrase, terminaselarge subunit, tail protein and holin) each was assigned a sequence type. All of the prophage integrases were identified to be tyrosine (XerC) recombinases and many of their respective attP/attR sequences were identified. The mosaic nature of E. faecium prophage genome sequence types supports previous hypotheses that extensive genetic recombination drives chimeric phage types.
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