Antioxidant and Inhibition of Elastase Effect of Scutellarein and Apigenin

Leo Tjermin, I Nyoman Ehrich Lister, Ali Napiah Nasution, Ermi Girsang

Abstract


Aging process in the skin was begun from the time when one is born, especially skin. Cutaneous aging can be caused by intrinsic and/or extrinsic factors. Wrinkles, thinning, and roughening of skin are part of skin aging. It becomes important looking for natural sources to prevent aging process. Apigenin and Scutellarein are potential phytochemistry which have antioxidant and anti-elastase effects. The purpose of this study is investigate antioxidant and anti-elastase effects of Apigenin and Flavonoid. Determination of Antioxidant Activity of Apigenin and Scutellarien was using FRAP Methods while anti-elastase activity was determined by inhibition of Elastase from Porcine Pancreas. The result of this study was expressed by Mean ± SD and analyzed by One Way analysis of variance (ANOVA) and Turkey HSD Post Hoc Test as a Further Analysis. Both of Scutellarein or Apigenin had the most potent antioxidant activity at the highest concentration (50 µg/ml). The FRAP Activity of Scutellarein or Apigenin at Highest concentration are 250.22 ±2.3 and 29.05 ±5.88 µg Fe (II), respectively. Antioxidant activity of Scutellarein are more potent than apigenin at various concentration and was shown differences at P value < 0.05. Same like antioxidant activity, anti-elastase activity of Scutellarein and apigenin has most potent inhibition effect at the highest concentration (66.67 µg/ml) which are 87.96 ± 1.63 and 28.33 ± 4.45 %, respectively while at various concentration of apigenin and scutellarein had differences percent inhibition of elastase at P value < 0.05. Scutellarein and Apigenin had antioxidant and anti-elastase activity. Each concentration of scutellarein had more potent antioxidant and anti-elastase activity than apigenin.


Keywords


Apigenin; Scutellarein; Antielastase; Inhibition of Elastase; Anti-elastase; Antioxidant; FRAP Methods; Elastase from Porcine Pancreas.

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