An Association of Prolactin Gene Polymorphisms with Some Milk Traits in Women
Abstract
We investigated the effects of prolactin gene polymorphisms on milk contents in women. The main aim of this work is to determine the genotypes of prolactin and its relationship with some milk chemical contents in women. Genotyping was carried out at Molecular Genetic Laboratory at the College of Agriculture, whereas biochemical assays were performed at the Department of Diseases Analyses at the South Technical University. Blood samples were collected for the prolactin-related gene. DNA was extracted from fifty candidate women. The extra-pituitary prolactin gene promoter 1149 G/T was subjected to XapI restriction enzyme. In this analysis PRL-Pxa, I products result in three genotypes TT, TG and GG, as well as the population, is under Hardy-Weinberg equilibrium. Our results showed that the highest milk fat yield, milk protein, lactose and sold not fat (SNF) materials percentages were obtained by the genotype GG.
References
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[20] Guzman-Guzman, I.P., Parra-Rojas, I., Oregón-Romero, E., Ledezma-Lozano, and IY Palafox-Sánchez, CA “The PADI4 haplotypes are associated with anti-CCP levels in rheumatoid arthritis from Western Mexico”. Clin Chem Lab Med 49, S205, Special suppl. 32, 2011.
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[3] Sinha, Y.N. “Structural variants of prolactin: occurrence and physiological significance”. Endocr Rev 16, pp 354–369, 1995.
[4] Nyante, SJ, Faupel-Badger, JM, Sherman, ME, Pfeiffer, RM, Gaudet, MM, Falk, RT, Andaya, et al “Genetic variation in PRL and PRLR, and relationships with serum prolactin levels and breast cancer risk: results from a population based case-control study in Poland”. Breast Cancer Research, 13, R42, pp 1-11, 2011.
[5] Williams, J.G.K., Kubelik, A.R, Liak, KJ Rafalski, J and Tingey, SV “DNA polymorphisms amplified by arbitrary primers are useful as enatic marker”. Nucleic Acids Research. 18, pp: 6531-6535, 1990.
[6] AL– Azzawie, AF “Extra pituitary prolactin –1149 G/T promoter polymorphism in some rheumatoid arthritis patients”. Al- Kindy College Medical Journal, Vol.11 No. 1, pp 40-44, 2015.
[7] Yeh FC Yang RC and Boyle T POPGENE, Version 1.31. “Microsoft Windows-based Freeware for Population Genetics Analysis”. Molecular Biology and Technology Center, University of Alberta, Canada. 1999.
[8] SPSS. “Statistical Packages of Social Sciences”. Version 15. USA 2006.
[9] Falconer, DS “Introduction to Quantitative Genetics”. 2ed Longman, UK. . 1981.
[10] Treadwell, E.L., Wiley, K., Word, B., Melchior, W., Tolleson, W.H., et al “Prolactin and Dehydroepiandrosterone Levels in Women with Systemic Lupus Erythematosus: The Role of the Extrapituitary Prolactin Promoter Polymorphism at ?1149G/T”. Journal of Immunology Research Volume Article ID 435658, 10 p 2015.
[11] Neidhart, M, Gay, RE, Gay, S “Prolactin and prolactin-like polypeptides in rheumatoid arthritis”. Biomed Pharmacotherapy 53, pp 218-222, 1999.
[12] Stevens, A, Newton, P, Anderson, NR, Gama, R “Characterization of a prolactin gene polymorphism and its associations with systemic lupus erythematosus”. Arthritis Rheum. 44 (10): pp 2358–2366, 2001.
[13] Mellai, M., Miller, A., Shtiller, R., Touby, E “Prolactin and prolactin receptor gene polymorphisms in multiple sclerosis and systemic lupus erythematosus”. Hum. Immunology 64, pp 274–284, 2003.
[14] Fojtíková, M, Radim, B, Jiri, VV “Polymorphism of the extra-pituitary prolactin promoter and systemic sclerosis”. Rheumatol. Int. 30 (12), pp 1691–1693, 2010.
[15] Montgomery, DW, Steven, R, Leuis, “A Prolactin production by immune cells”. Lupus 10, pp 665-675, 2001.
[16] Bole-Feysot, C, Goffin, V, Edery, M, Binart, N, and Kelly, PA “Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice”. Endocr. Rev, vol.19, no.3, pp 225–268, 1998.
[17] Goffin, V., Binart, N., Touraine, P., and P.A. Kelly “Prolactin: the new biology of the old hormone”. Annual Review of Physiology, vol.64, pp 47–67, 2002.
[18] Lee, Y.C., Sulli, A., Fasciolo, D. “The PRL –1149 G/T polymorphism and rheumatoid arthritis susceptibility”. Arthritis Rheum, 60 (5), pp 1250–1254, 2009.
[19] Kochi, Y., Suzuki, A., Yamada, R., Yamamoto, K. “Ethnogenetic heterogeneity of rheumatoid arthritis-implications for pathogenesis”. Nat. Rev. Rheumatol, 6, pp 290–295, 2010.
[20] Guzman-Guzman, I.P., Parra-Rojas, I., Oregón-Romero, E., Ledezma-Lozano, and IY Palafox-Sánchez, CA “The PADI4 haplotypes are associated with anti-CCP levels in rheumatoid arthritis from Western Mexico”. Clin Chem Lab Med 49, S205, Special suppl. 32, 2011.
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Published
2018-09-25
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FA, A., AY, A., & NM, A. (2018). An Association of Prolactin Gene Polymorphisms with Some Milk Traits in Women. American Scientific Research Journal for Engineering, Technology, and Sciences, 47(1), 21–27. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/4240
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