The Working Mechanism of CRISPR/Cas9 Genome Editing in Filamentous Fungi and its Industrial Applications
Keywords:
CRISPR, filamentous fungi, regulation of gene, aspergillus oryzae, aspergillus niger, aspergillus nidulansAbstract
Understanding of regulation of gene and its functions sequence which is coding the gene and genome manipulation are very crucial. For this understanding conventional methods in many filamentous fungi are either non-functional or ineffective. Filamentous fungi plays major role in agriculture, industrial production and in medical field. A broad applications and fast development of system of CRISPR/Cas9 method has given the basic outline to comply this technology of editing of gene for many filamentous fungi. In this review, by focusing the applications of CRISPR/Cas9 in research as well as in industries we give the brief introduction of the CRISPR/Cas9 system. We also give the brief description of overall review of editing of genome, describe the construction of vector, off targeted effects and different methods of transformation of the plasmid
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