Formulation and In-vitro Evaluation of Tolterodine Tartrate Tablets by Using High Performance Liquid Chromatographic (HPLC)


  • Sumitra Shrestha M.Pharm (Clinical pharmacy) Pokhara University, Assistant Quality Assurance Manager Divine Healthcare Pvt. Ltd. Bharatpur-16, Nepal
  • Sangit Maharjan B.Pharm Shree medical & Technical College, Production Officer Divine Healthcare Pvt. Ltd. Bharatpur-16
  • Nanda Kishor Bhatta M.Pharm (Industrial Pharmacy, Kathmandu University, Associate professor, Shree Medical and Technical College, Bharatpur, Chitwan
  • Arjun Gaire B.Pharm, Shree Medical & Technical College, QA Officer, Kasturi Pharmaceuticals Pvt. Ltd.Bharatpur-16, Chtiwan.
  • Naresh Thapa B.Pharm Dhaka University Bangladesh, Quality Assurance Manager Divine Healthccare Pvt. Ltd. Bharatpur-16, Nepal
  • Dr. Jeevan Raj Shrestha B.SC Microbiology (Tribhuvan University), MBBS (Manipal College of Medical Sciences, Pokhara) Kathmandu University, Medical Officer Barhabise Primary Health Centre Barhabise-9, Nepal


Tolterodine tartrate, HPLC, Assay, Dissolution


Tolterodine tartrate, is a new, potent and competitive muscarinic receptor antagonist in clinical development for the treatment of urge incontinence and other symptoms of unstable bladder. The purpose of this study is to formulation and invitro evaluation of Tolterodine tartrate by high performance liquid chromatography with ultraviolet detection (HPLC-UV). A simple, rapid, and sensitive high-performance liquid chromatographic method was developed and evaluated for invitro formulation of Tolterodine tartrate Tablets. Tablets were analysed by measuring different parameters: lubricated granules content of Tolterodine tartrate having bulk density, tap densities and angle of r

content uniformity, assay and related substances. Separation of Tolterodine tartrate was achieved within a single chromatographic run on 5µm 4.6x250mm with UV detection at 280 nm, under isocratic conditions, using Acetonitrile and A mixture of 65 volumes of buffer solution prepared by mixing 2.2 ml of orthophosphoric acid to 1000 ml with water, adjusted to pH 3.0 with triethylamine in 35:65 ratio with a flow rate of 1.5 ml/min. From the results, it was clear that designed formulations among f7 displayed drug release in the range of 55.66% to 102.067% in 10 min, which showed improved invitro dissolution rate compared to other formulations as well as others parameters were found to be good as compared to other formulations. Similarly, the average content of formulation f7 was found to be 104.58% and Related substances should comply the test. Assays of f7 were found to be 96.04%, the limit is 90% - 110% of the label claim having weight variation range from  82.50 mg-91.50 mg.

epose And flim coated Tolterodine tartrate tablets having friability, thickness, hardness, weight variation, invitro dissolution,


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How to Cite

Shrestha, S., Maharjan, S. ., Bhatta, N. K. ., Gaire, A. ., Thapa , N. ., & Shrestha , D. J. R. . (2021). Formulation and In-vitro Evaluation of Tolterodine Tartrate Tablets by Using High Performance Liquid Chromatographic (HPLC). American Scientific Research Journal for Engineering, Technology, and Sciences, 77(1), 95–104. Retrieved from