Antibiotic Resistant Genes in Multidrug-Resistant Extended-Spectrum ?-lactamase-Producing E. coli Isolated from Children's Samples

Authors

  • Salam sliai Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza, Egypt.
  • Aly E. Abo-Amer Department of Botany and Microbiology, Faculty of Science, Sohag University, Sohag (82524), Egypt.
  • Hani Moubasher Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza, Egypt.

Keywords:

E. coli, MDR, ESBLs, Children

Abstract

Antimicrobial resistance was declared by the World Health Organization (WHO) in 2014 as the greatest threat for human and veterinary medicine.The development of resistance in E. coli may be due to haphazard use of antibiotics, plasmid-mediated genes,i.e. blaCTX-M, blaSHV, blaOXA. This work was aimed to detect the antibiotic resistant genes in multidrug-resistant extended-spectrum ?-lactamase-producing E. coli from samples of children. 90 samples from chidren were collected. blaTEM, blaSHV, blaOXA, blaCTX-M and blaVEB resistance genes in drug-resistant E. coli were investigated. The blaTEM, blaSHV and blaCTX genes were detected in E.coli isolates. blaTEM genes were found to be in all isolates. The blaSHV gene was detected in 14.28% of the isolates. The blaCTX-M gene was detected in 71.42% of the isolates blaOXA and blaVEB genes were not detected in any sample.The resistant genes sequences of the E.coli isolates from urine, blood, skin ande ndotracheal tube were deposited in the DDBJ/EMBL/GenBank nucleotide sequence databases.The resistance genes sequences of the Escherichia isolates are greatest closely associated to those of Escherichia coli.

References

. R. Podschun, U.Ullmann, " nosocomial pathogens: epidemiology: taxonomy, typing methods, and pathogenicity factors" Clinical Microbiology Reviews, vol. 11, p. 603-589,1998.

. N. Allocati, M. Masulli, MF. Alexeyev, C. Di Ilio,"Escherichia coli in Europe: an overview" International Journal of Environmental Research and Public Health, vol. 10, p. 6235-6254,2013.

. AM. Fody, L. Boubou, A. Moussa, HI. Bawa, Daouda. A, Sidikou. R, AB. Traoure, N. Barro, "Phenotypic Detection of ESBL in multidrug resistant E. coli from clinical isolates in Niamey, Niger" African Journal of Microbiology Research, vol. 11, p .713-717, 2017.

. WHO, "Diarrhoea",2012.

. YB. Ngwai, SD. Gyar, GRI. Pennap, MD. Makut, D. Ishaleku, SM. Corosi, IH. Nkene, N. Uzoamaka, "Antibiogram of non-sorbitol fermenting Escherichia coli from sources and stool in Keffi, Nigeria" NSUK Journal of Science and Technology, vol. 4, 2014.

. N. Dayan, H. Dabbah, I. Weissman, I. Aga, L. Even, D. Glikman, "Urinary tract infections caused by community-acquired extended spectrum beta lactamase producing and nonproducing bacteria: a comparative study" The Journal of Pediatrics, vol. 163, p. 1417-1421, 2013.

. S. Karanika, T. Karantanos, M. Arvantis, C. Grigoras, E. Mylonakis, "Fecal colonic and risk factors among healthy individuals: a systematic review and metanalysis" Clinical Infectious Diseases, vol. 63, p. 308-310, 2016.

. A. Brolund, " Overview of ESBL-producing Enterobacteriaceae from a Nordic perspective" Infection Ecology & Epidemiology, vol. 4, p. 3402-3410, 2014.

. A. Alqasim, A. Abu Jaffal, AA. Alyousef, " Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia" International Journal of Microbiology, vol. 2018, p. 1-10, 2018.

. Bo. Ogunbosi, C. Moodley, P. Naicker, J. Nuttall, C. Bamford, B. Eley, "Colonisation and carbapenem-resistant Enterobacterales in with extended spectrum beta-lactamase-producing children admitted to a paediatric referral hospital in South Africa" PLoS ONE, vol. 15, p. 0241776, 2020.

. S. Shrestha, R. Amatya, R. Dutta," Prevalence of extended spectrum beta lactamase (ESBL) production in gram negative isolates from pyogenic infection in tertiary care hospital of eastern Nepal" Nepal Medical College Journal, vol.13, p. 186-189, 2011.

. A. Birgy, F. Madhi, C. Jung, C. Lev, A. Cointe, Ph. Bidet, CA. Hobson, S. Bechet, E. Sobral, H. Vuthien, A. Ferroni, S. Aberrane, G. Cuzon, L. Beraud, V. Gajdos, E. Launay, D. Pinquier, H. Haas, M. Desmarest, MA. Dommergues, R. Cohen, S. Bonacorsi, " Diversity and trends in population structure of ESBL-producing Enterobacteriaceae in febrile urinary tract infections in children in France from 2014 to 2017" Journal of Antimicrobial Chemotherapy, vol. 75, p. 96-105,2020.

. L. Pallecchi, M. Malossi, A. Mantella, E. Gotuzzo, C. Trigoso, A. Bartoloni "Detection of CTX-M-type beta-lactamase genes in fecal Escherichia coli isolates from healthy children in Bolivia and Peru" Antimicrobial Agents and Chemotherapy, vol. 48, p. 4556–61, 2004.

. A. Bartoloni, L. Pallecchi, E. Riccobono, A. Mantella, D. Magnelli, T. Di Maggio "Relentless increase of resistance to fluoroquinolones and expanded-spectrum cephalosporins in Escherichia coli: 20 years of surveillance in resource-limited settings from Latin America" Clinical Microbiology and Infection, vol. 19, p. 356-361, 2013.

. L. Pallecchi, A. Bartoloni, C. Fiorelli, A. Mantella, T. Di Maggio, H. Gamboa, "Rapid dissemination and diversity of CTX-M extended-spectrum beta-lactamase genes in commensal Escherichia coli isolates from healthy children from low-resource settings in Latin America" Antimicrobial Agents and Chemotherapy, vol.51, p.2720- 2725, 2007.

. E. Ruppe, PL. Woerther, A. Diop, AM. Sene, A. Da Costa, G. Arlet,"Carriage of CTX-M-15-producing Escherichia coli isolates among children living in a remote village in Senegal" Antimicrobial Agents and Chemotherapy, vol. 53, p. 3135–3137, 2009.

. D. Paterson, R. Bonomo," Extended-Spectrum β-Lactamases: A Clinical Update" Clinical Microbiology Reviews, vol. 18, p. 657–686, 2005.

. S. Karanika, T. Karantanos, M. Arvantis, C. Grigoras, E. Mylonakis, "Fecal colonization with extended-spectrum beta-lactamase-producing Enterobacteriaceae and risk factors among healthy individuals: a systematic review and metanalysis" Clinical Infectious Diseases, vol. 63, p. 310-308, 2016.

. R. Canton, A. Novais, A. Valverde, E. Machado, L. Peixe, F. Baquero," Prevalence and spread of extended-spectrum b-lactamase-producing enterobacteriaceae in Europe" Clinical Microbiology and Infection, vol. 14, p. 144-153, 2008.

. K. Bush, "Extended-spectrum b-lactamases in North America, 1987-2006" Clinical Microbiology and Infection, vol. 14, p. 134-143, 2008.

. A. Chanawong, FH. M'Zali, J. Heritage, JH. Xiong, PM. Hawkey, "Three cefotaximases, CTX-M-9, CTX-M-13, and CTX-M14, among Enterobacteriaceae in the People's Republic of China" Antimicrobial Agents and Chemotherapy, vol. 46, p. 630-637, 2002.

. JH. Chia, C. Chu, LH. Su, CH. C, AJ. Kuo, CF. Sun, "Development of a multiplex PCR and SHV melting-curve mutation detection system for detection of some SHV and CTX-M b-lactamases of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae in Taiwan" Journal of Clinical Microbiology, vol. 43, p. 4486-4491, 2005.

. JDD. Pitout, KB. Laupland, "Extended-spectrum b-lactamase-producing Enterobacteriaceae: an emerging public-health concern" The Lancet Infectious Diseases, vol. 8, p. 159-166, 2008.

. K. Bush, GA. Jacoby, "Updated functional classification of blactamases" Antimicrobial Agents and Chemotherapy, vol. 54, p. 969-976, 2010.

. SK. Dubois, MS. Marriott, SG. Amyes, "TEM and SHV derived extended-spectrum b-lactamases: relationship between selection, structure and function" Antimicrobial Agents and Chemotherapy, vol. 35, p. 7-22, 1995.

. PA. Bradford, "Extended-spectrum b-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat" 2001.

. DJ. Brenner, NR. Krieg, JT. Staley, GM. Garrity, "Bergey's Manual of Systematic Bacteriology", 2nd edition. New York, Springer,2005.

. TV. Nguyen, PL. Van, CL. Huy, KN. Gia, A. Weintraub, "Etiology and epidemiology of diarrhea in children in Hanoi, Vietnam" International Journal of Infectious Diseases. vol. 10, p. 298-308, 2006.

. A. Abo Amer, M Shobrak, A. Altalhi, "Isolation and antimicrobial resistance among Escherichia coli isolated from farm chickens in Taif province, Saudi Arabia" Journal of Global Antimicrobial Resistance. vol. 15, p. 65-68.

. K. Kayastha, B. Dhungel, Sh. Karki, B. Adhikari, M. Raj Banjara, K. Raj Rijal, P. Ghimire, "Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella Species in Pediatric Patients Visiting International Friendship Children’s Hospital, Kathmandu, Nepal" Auckland DHB Infectious Diseases, vol. 13, p. 1178633720909798, 2020.

. N. Thampi, J. Bowes, R. Melano, N. Tijet, R. Slinger, " Clinical and Genetic Characteristics of Extended-Spectrum Beta-Lactamase–Producing Enterobacteriaceae Among Canadian Children 2020" PLoS One, vol. 1, p. 141-168, 2020.

. BO. Ogunbosi, C. Moodley, P. Naicker, J. Nuttall, C. Bamford, B. Eley, " Colonisation with extended spectrum beta-lactamase-producing and carbapenem-resistant Enterobacterales in children admitted to a paediatric referral hospital in South Africa" PLOS ONE, vol. 15, p. e0241776, 2020.

. T. Yamanaka, Y. Horikoshi," CTX-M group gene distribution of extended spectrum beta-lactamase-producing Enterobacteriaceae at a Japanese Children's hospital" Journal of Infection and Chemotherapy, vol. 26, p. 1005-1007, 2020.

. R. Pandit, B. Awal, S. Shreekhanda, G. Joshi, B. Rijal, N. Prasad, P. corresponding," Extended-Spectrum β-Lactamase (ESBL) Genotypes among Multidrug-Resistant Uropathogenic Escherichia coli Clinical Isolates from a Teaching Hospital of Nepal" Interdisciplinary Perspectives on Infectious Diseases, vol. 2020, p. 6525826, 2020.

. E. Jafari, S. Mostaan, S. Bouzari "Characterization of Antimicrobial Susceptibility, Extended-Spectrum β-Lactamase Genes and Phylogenetic Groups of Enteropathogenic Escherichia coli Isolated from Patients with Diarrhea" Osong Public Health and Research Perspectives, vol. 11, p. 327–333, 2020.

. N. Eltai, A. Al Thani, S Al Hadid, K. Al Ansar, H. Yassine, " Antibiotic resistance and virulence patterns of pathogenic Escherichia coli strains associated with acute gastroenteritis among children in Qatar" BMC Microbiology, vol. 20, p. 54, 2020.

. A. Zakou, I. Nkene, R.Abimiku, I. Yahaya, B. Bassey, Y. Ngwai, " Antimicrobial Resistance Profile and Detection of Extended Spectrum and Amp C β-Lactamase Resistance Genes in Escherichia coli Isolated from Diarrheic Children in Lafia, Nasarawa State, Nigeria" South Asian Journal of Research in Microbiology, vol. 7, p. 15-27, 2020.

. M. Alikhani, Z. Tabar, F. Mihani, E. Kalantar, P. Karami, M. Sadeghi, Sh. Ahdi Khosroshahi, S. Farajnia, " Antimicrobial Resistance Patterns and Prevalence of blaPER-1 and blaVEB-1 Genes Among ESBL-producing Pseudomonas aeruginosa Isolates in West of Iran" Jundishapur Journal of Microbiology, vol. 7, p. e8888, 2014.

Downloads

Published

2020-12-19

How to Cite

sliai, S. ., Abo-Amer , A. E. ., & Moubasher , H. . (2020). Antibiotic Resistant Genes in Multidrug-Resistant Extended-Spectrum ?-lactamase-Producing E. coli Isolated from Children’s Samples. American Scientific Research Journal for Engineering, Technology, and Sciences, 74(2), 101–113. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/6511

Issue

Section

Articles