Inoculation with Pseudomonas Pseudoalcaligenes Lead to Changes in Plant Sugar Metabolism and Defense That Enhance Tolerance Against the Pathogenic Fungus Sclerotium Rolfsii
AbstractCertain Pseudomonas species promote in plants an induced systemic response (ISR), which results in pathogenic disease reduction. This is energetically expensive, implies a redistribution of sugars, and involves several enzymes such as cell-wall invertase (cwINV). The present study aimed to evaluate the role of soluble sugars and cwINV activity in the ISR of Pseudomonas pseudoalcaligenes-primed tomato plants challenged with Sclerotium rolfsii. Disease severity of infected plants was 100%, whereas that of primed plants was 43%. At 24 h after challenge, infected plants showed higher cwINV activity, increased LIN6 and SUS3 mRNA levels, upregulation of the defense marker gene PR1b1, no changes in PR2 and PR3 mRNA levels, and almost unchanged sugar content. Instead, primed plants displayed a lower induction of cwINV activity and gene expression, slightly increased PR2 and PR3 mRNA levels, and increased leaf fructose content. Cytokines also induced LIN6 expression and cwINV activity. Altogether, these results reveal that P. pseudoalcaligenes triggers changes both in sugar metabolism and plant defense, leading to enhanced tolerance against Sclerotium rolfsii.
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