Comparison of Burn Wound Healing Ability between Ethyl Acetate Extract Gel of Betel Nut (Areca catechu) and Snake Fruit Seed (Salacca zalacca) in Rats

  • Ferra Sherliana Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • Ermi Girsang Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • Ali Napiah Nasution Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • I Nyoman Ehrich Lister Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
Keywords: Betel nut, Snake fruit, Ethyl Acetate, Gel


The burn is the most common type of trauma that is found over the world. Betel nut and snake fruit belong to the family of Arecaceae have various phytochemicals that have antioxidant activity to accelerate the wound healing process. This study was aimed to explore the wound healing effect of ethyl acetate extract from betel nut and snake fruit. This study used 20 rats that divided into four groups include Standard (Bioplaceton©), Control, Gel of Snake fruit seed, and Gel of Betel nut. The gel was formulated from the ethyl acetate extract of snake fruit seed and betel nu by the maceration process. A steel plate conducted the burn under ketamine anesthesia. The parameters that were evaluated were wound contraction and epithelialization period. The result of this study showed that the wound contraction showed a significant difference up to 4 days of observation (P-value < 0.05). While, the group of standard, gel of snake fruit seed, and gel of betel nut have a significant difference in epithelialization period against the control group (P-Value < 0.05). Overall, both of the gel extracts have potential acceleration burn wound healing. However, the gel of betel nut is more effective than the gel of snake fruit seed.


. S. ur Rehman et al., “The Distribution and Determinants of Burn Injuries at Jinnah Burn and Reconstructive Surgery Centre, Lahore,” Proceedings, vol. 32, no. 2, pp. 31–35, 2018.

. N. Primadina, “Epidemiologi Kasus Bedah Plastik Di Rsud Syarifah Ambami Rato Ebhu Bangkalan, Penelitian Retrospektif Dua Tahun,” Med. Heal. Sci. J., vol. 1, no. 2, pp. 41–46, 2018.

. R. Suzan and D. E. Andayani, “Tata laksana nutrisi pada pasien luka bakar listrik,” JMJ, vol. 5, no. 1, pp. 1–13, 2017.

. R. Rajamani, K. Arts, K. S. Kuppusamy, and K. Arts, “Preliminary Phytochemical Screening of Aqueous Extract of Betel Nut and Betel Leaves,” Int. J. Biosci. Nanosci., vol. 3, no. 1, pp. 14–18, 2016.

. M. S. M. Saleh et al., “Salacca zalacca: A short review of the palm botany, pharmacological uses and phytochemistry,” Asian Pac. J. Trop. Med., vol. 11, no. 12, pp. 645–652, 2018.

. M. Bharat, D. K. Verma, V. Shanbhag, R. S. Rajput, D. Nayak, and A. Amuthan, “Ethanolic extract of oral Areca catechu promotes burn wound healing in rats,” Int. J. Pharm. Sci. Rev. Res., vol. 25, no. 2, pp. 145–148, 2014.

. S. Istiana, “Formulasi sediaan gel basis Na-CMC ekstrak etanol daun cocor bebek (Kalanchoe pinnata (Lmk.) Pers.) sebagai penyembuh luka bakar pada kelinci,” Universitas Muhammadiyah Surakarta, 2016.

. W. Widowati et al., “Antioxidant and antidiabetic potential of Curcuma longa and its compounds,” Asian J. Agric. Biol., 2018.

. D. K. Verma, M. Bharat, D. Nayak, T. Shanbhag, V. Shanbhag, and R. S. Rajput, “Areca catechu : Effect of topical ethanolic extract on burn wound healing in albino rats,” Int. J. Pharmacol. Clin. Sci., vol. 1, no. 3, pp. 74–78, 2012.

. F. Handayani, E. Siswanto, and L. Pangesti, “Uji Aktivitas Ekstrak Etanol Gambir (Uncaria gambir Roxb.) terhadap Penyembuhan Luka Bakar pada Kulit Punggung Mencit Putih Jantan (Mus musculus),” J. Ilm. Manuntung, vol. 2, no. 2, pp. 154–160, 2016.

. E. Girsang et al., “Chemical Constituents of Snake Fruit (Salacca zalacca (Gaert.) Voss) Peel and in silico Anti-aging Analysis,” Mol. Cell. Biomed. Sci., 2019.

. E. Girsang et al., “In silico analysis of phytochemical compound found in snake fruit (Salacca zalacca) peel as anti-aging agent,” Thai J. Pharm. Sci., 2019.

. L. C. Agra, J. N. S. Ferro, F. T. Barbosa, and E. Barreto, “Triterpenes with healing activity: A systematic review,” J. Dermatolog. Treat., vol. 26, no. 5, pp. 465–470, 2015.