Scavenge ABTS and Inhibition of Elastase Enzyme Activity from Ethanol Extract of Pineapple (Annas cosmusus (L.) Merr) Core

  • Lenny Wijaya Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • I Nyoman Ehrich Lister Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • Edy Fachrial Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • Ermi Girsang Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
Keywords: Elastase Inhibition, ABTS, Pineapple core, Luteolin

Abstract

The exact cause of aging is still unknown, there are some theories which has been directed. Excess ROS (Reactive oxygen species) or UV radiation can precipitate activation of elastase which degrades elastin. This study was aimed to investigate anti-elastase and antioxidant activity of ethanol extract from pineapple core. Determination of Antioxidant activity was using ABTS Methods while Inhibition of Elastase assay was using Elastase Enzyme from porcine pancreases. The result of this study was express by Mean ± SD and analysed by One-Way ANOVA test and followed by the Post Hoc Test with the Tukey HSD test, while IC50 was determined by simultaneous analysis of linear regression. At the highest concentration (50 ug/ml), The scavenge activity of ABTS’ were 36.13 ± 2.82 % for ethanol extract of pineapple core and 93.91 ± 3.25 %  for luteolin compound. Same as Scavenge ABTS Activity, at the highest concentration (66.67 ug/ml), the elastase inhibition activities were 78.73 ±3.08 % for ethanol extract of pineapple core and 86.30 ± 1.78 % for luteolin compound. The result of Post Hoc test for ABTS activity and inhbition of elastase was shown significant differences (P value < 0.05) of percentage activity at various concentration. Based on IC50 value of Ethanol extract from pineapple core that antioxidant activity (IC50: 72.73±4.31) was strong while inhibition of elastase enzyme (IC50:16.79±1.62) was very strong. Antioxidant and antielastase activity of ethanol extract from pineapple core aren’t potent as luteolin compound.

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Published
2020-06-26
Section
Articles