Scavenge ABTS and Inhibition of Elastase Enzyme Activity from Ethanol Extract of Pineapple (Annas cosmusus (L.) Merr) Core

Authors

  • Lenny Wijaya Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • I Nyoman Ehrich Lister Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • Edy Fachrial Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • Ermi Girsang Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia

Keywords:

Elastase Inhibition, ABTS, Pineapple core, Luteolin

Abstract

The exact cause of aging is still unknown, there are some theories which has been directed. Excess ROS (Reactive oxygen species) or UV radiation can precipitate activation of elastase which degrades elastin. This study was aimed to investigate anti-elastase and antioxidant activity of ethanol extract from pineapple core. Determination of Antioxidant activity was using ABTS Methods while Inhibition of Elastase assay was using Elastase Enzyme from porcine pancreases. The result of this study was express by Mean ± SD and analysed by One-Way ANOVA test and followed by the Post Hoc Test with the Tukey HSD test, while IC50 was determined by simultaneous analysis of linear regression. At the highest concentration (50 ug/ml), The scavenge activity of ABTS’ were 36.13 ± 2.82 % for ethanol extract of pineapple core and 93.91 ± 3.25 %  for luteolin compound. Same as Scavenge ABTS Activity, at the highest concentration (66.67 ug/ml), the elastase inhibition activities were 78.73 ±3.08 % for ethanol extract of pineapple core and 86.30 ± 1.78 % for luteolin compound. The result of Post Hoc test for ABTS activity and inhbition of elastase was shown significant differences (P value < 0.05) of percentage activity at various concentration. Based on IC50 value of Ethanol extract from pineapple core that antioxidant activity (IC50: 72.73±4.31) was strong while inhibition of elastase enzyme (IC50:16.79±1.62) was very strong. Antioxidant and antielastase activity of ethanol extract from pineapple core aren’t potent as luteolin compound.

References

. Y. Liu, J. Long and J. Liu, "Mitochondrial free radical theory of aging: who moved my premise?," Geriatr Gerontol Int. , vol. 14, no. 4, pp. 740-749, 2014.

. K. Jin, "Modern Biologival Theories of Aging," Aging Dis, vol. 1, no. 2, pp. 72-64, 2010.

. A. Nurrochmad, Wirasti, A. Dirman, E. Lukitaningsih, A. Rahmawati and N. Fakhrudin, "Effects of Antioxidant, Anti-Collagenase, AntiElastase, Anti-Tyrosinase of The Extract and Fraction From Turbinaria decurrens Bory.," Indonesian J. Pharm, vol. 29, no. 4, pp. 188-197, 2018.

. S. Zhang and E. Duan, "Fighting against Skin Aging: The Way from Bench to Bedside," Cell Transplantation, vol. 27, no. 5, pp. 729-738, 2018.

. S. R. Varmal, A. Mishral, M. Vijayakumar and R. Paramesh, "Anti-skin Ageing Phytochemicals in Cosmetics; An Appraisal," H&PC Today - Household and Personal Care Today, vol. 12, no. 2, pp. 20-23, 2017.

. S. F. Nabavi, NadyBraidy, OlgaGortzi, EduardoSobarzo-Sanchez, M. Daglia, K. Skalicka-Woźniak and S. M. Nabavi, "Luteolin as an anti-inflammatory and neuroprotective agent: A brief review," Brain Research Bulletin, vol. 119, pp. 1-11, 2015.

. V. V. Irda Fidrianny and M. Insanu, "Antioxidant Potential of Different Parts of Bogor Pineapple (Ananas Comosus (L) Merr. Var. Queen) Cultivated in West Java-Indonesia," Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 1, pp. 129-133, 2018.

. W. Widowati, R. M. Widyanto, W. Husin, H. Ratnawati, D. R. Laksmitawati, B. Setiawan, D. Nugrahenny and I. Bachtiar, "Green tea extract protects endothelial progenitor cells from oxidative insult through reduction of intracellular reactive oxygen species activity," Iran J Basic Med Sci, vol. 17, no. 9, pp. 702-709, 2014.

. W. Widowati, N. Fauziah, H. Herdiman, M. Afni, E. Afifah, H. S. W. Kusuma, H. Nufus, S. Arumwardana and D. D. Rihibiha, "Antioxidant and Anti Aging Assays of Oryza Sativa Extracts, Vanillin and Coumaric Acid," Journal of Natural Remedies, vol. 16, no. 3, pp. 88-99, 2016.

. W. Widowati, A. P. Rani, R. A. Hamzah, S. Arumwardana, E. Afifah, H. S. W. Kusuma, D. D. Rihibiha, H. Nufus and A. Amalia, "Antioxidant and antiaging assays of Hibiscus sabdariffa extract and its compounds," Nat. Prod. Sci, vol. 23, no. 3, pp. 192-200, 2017.

. W. Widowatia, W. J. B, S. Nadya, A. Amalia, S. Arumwardana, H. S. W. Kusuma and Y. Arinta, "Antioxidant and Antiaging Activities of Jasminum Sambac Extract, and its Compounds," J. Reports Pharmaceutic. Sci, vol. 7, no. 3, pp. 270-285, 2018.

. A. Taskeen, I. Naeem, S. Bakhtawar and T. Mehmood, "Comparative Study of Flavonoids in Fruits and Vegetables with Their Products using Reverse Phase High Performance Liquid Chromatography (RP-HPLC)," EJEAFChe, vol. 9, no. 8, pp. 1372-1377, 2010.

. S. Bhagwat, D. B. Haytowitz and J. M. Holden, USDA Database for the Flavonoid Content of Selcted Foods, Maryland: U.S. Department of Agriculture, 2014.

. A. Menon, V. P. V and G. R, "Preliminary Phytochemical Analysis and Cytotoxicity Potential of Pineapple Extract on Oral Cancer Cell Lines," Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 2, pp. 140-143, 2016.

. I. Fidrianny, V. Virna and M. Insanu, "Antioxidant Potential of Different Parts of Bogor Pineapple (Ananas Comosus (L) Merr. Var. Queen) Cultivated in West Java-Indonesia," Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 1, pp. 129-133, 2018.

. E. Karimi, P. Mehrabanjoubani, M. Keshavarzian, E. Oskoueian, H. Z. Jaafar and A. Abdolzadeh, "Abdolzadeh A. Identification and quantification of phenolic and flavonoid components in straw and seed husk of some rice varieties (Oryza sativa L.) and their antioxidant properties," J Sci Food Agric, vol. 94, no. 11, pp. 2324-2330, 2014.

. A. Pouillot, L. L. Polla, P. Tacchini, A. Neequaye, A. Polla and B. Polla, "Natural Antioxidant and Their Effects on The Skin," in Formulating, Packaging, and Marketing of Natural Cosmetic Products First Edition, New York, John Wiley & Sons, Inc, 2011, pp. 239-257.

. T. S. Thring, P. Hilli and D. P. Naughton, "Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants`," BMC Complement Altern Med, vol. 9, no. 27, pp. 1-11, 2009.

. O. Makarenko and A. Levitsky, "Biochemical Mechanisms of Therapeutic and Prophylactic Effects of Bioflavonoids," Journal of Pharmacy and Pharmacology, vol. 4, pp. 451-456, 2016.

Downloads

Published

2020-06-26

How to Cite

Wijaya, L. ., Lister, I. N. E. ., Fachrial, E., & Girsang, E. (2020). Scavenge ABTS and Inhibition of Elastase Enzyme Activity from Ethanol Extract of Pineapple (Annas cosmusus (L.) Merr) Core. American Scientific Research Journal for Engineering, Technology, and Sciences, 70(1), 106–113. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/5716

Issue

Vol. 70 No. 1 (2020)

Section

Articles

License

Authors who submit papers with this journal agree to the following terms.