Ferric Reducing Antioxidant Power (FRAP) and Inhibition of Collagenase Enzyme Activity from Ethanol Extract of Pineapple (Ananas cosmus (L.) Merr) Core
Keywords:Collagenase Inhibition, FRAP, Pineapple Core, Luteolin
Reactive Oxygen Species (ROS) is one of aerobic respiration products in cells which cause aging. Pineapple core is able to protect skin healthy by reducing reactivity from ROS, inhibiting oxidation, absorbing UV light, suppressing enzyme activity (such as collagenase), reducing the formation of wrinkles on the skin and protecting the skin from aging. This study aimed to investigate antiaging effect of pineapple core by their antioxidant and anti-collagenase activity. Determination of Antioxidant activity was using FRAP Methods while Inhibition of Collagenase assay was using Collagenase Enzyme from Clostridium histolyticum. The result of this study was expressed by Mean ± SD and analysed by One-Way ANOVA test and followed by the Post Hoc Test with the Tukey HSD test while Inhibition of collagenase were followed to analyse by simultaneous analysis of linear regression then determined the value of Inhibition Concentration 50 (IC50). At the highest concentration (50 µg/ml) from each sample were shown highest FRAP activity were 149.92±0.75 µg Fe2+ for ethanol extract of pineapple core and 255.05±1.04 µg Fe2+ for luteolin. Same as FRAP Activity, Inhibition of Collagenase assay at the highest concentration (250 µg/ml) from each sample were shown highest percent of collagenase inhibition were 64.62 ± 0.73% for ethanol extract of pineapple core and 81.22 ± 1.00%for luteolin while IC50 value of collagenase inhibition activity are shown that ethanol extract of pineapple core has moderate inhibition activity. Whether FRAP activity or Inhibition of Collagenase activity aren’t potent as luteolin compound as comparison.
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