Effectiveness Test of Robusta Coffee (Coffea cenephora) Extract from North Sumatra in Collagen and Hydration Skin Level of Female Wistar Rattus norvegicus


  • Lora Coarnita Girsang Master of Biomedical Science, Faculty of Medicine, Prima Indonesia University, Medan, North Sumatera, Indonesia
  • Edy Fachrial Faculty of Medicine, University of Prima Indonesia, Medan, North Sumatera, Indonesia
  • I Nyoman Ehrich Lister Faculty of Medicine, University of Prima Indonesia, Medan, North Sumatera, Indonesia


Coffea Cenephora, Collagen, Hydration, Anti-Aging


Robusta coffee (Coffea canephora) is one of the Indonesian plants that have antioxidant compounds that can be used as cosmetic ingredients. The antiaging effect of the coffee extract can overcome the photoaging problem. The purpose of this study is to see the anti-aging effects of robusta coffee bean extract based on concentration variations of the female white rats. The study was conducted in a laboratory with pre and post control group design, from February to March 2019. 30 female white rats were divided into 5 groups and were sunbathed for 5 days, then were shaved 2x2 cm wide on the back and were given Robusta coffee bean extract cream 2.5%, 5%, 7.5%, 10%, and control. Applying cream twice a day for 4 weeks and changes were measured every 1 week for 4 weeks with a skin analyzer. The results were tested for the normality with the Shapiro-Wilk test and Levene's test. Then followed by Anova Repeated, Pearson Correlation and Multiple Linear Regression test. Data analysis was using SPSS 21.0. There were the highest increased of collagen and hydration levels with Coffea canephora 10% extract given, and variable concentrations and duration of administration have a positive effect. The highest increased of collagen level was in the Coffea canephora 10% extract with an average 91.3% and hydration level amounted to 86.09%.


Wasitaatmadja SM. 2013.Faal Kulit. In : Adhi D,editor. Ilmu Penyakit Kulit dan Kelamin.Jakarta : Fakultas Kedokteran Universitas Indonesia .p.7-8.

Sjerobabski-Masnec I, Situm M. 2010. Skin aging. Acta Clin Croat. Dec;49(4): 515-8. PubMed PMID:21830465.

Biniek,K.,Kemal.L & Reinhold.H.D. 2012. Solar UV radiationreduces the barier function of human skin. In : John W.H (ed) Proceedings of the National Academy of Sciences of the United States of America. 109 (42), National Academy of Sciences, pp 17111–17116. doi: 10.1073/pnas.1206851109.

McLafferty E, Alistair F, and Hendry C. 2012. The integumentary system: anatomy, physiology and function of skin . Nursing Standart ; Vol. 27 (3): p.35-42.

Alhana, Pipih S, Kustiariyah. 2015. Extraction and Characterization of Collagen from Sea Cucumber Flesh.JPHPI. Aug; Vol 18(2): 150-161.

Barel A.O., Paye M. and Maibach H.I. 2009. Handbook of Cosmetic Science and Technology. 3rd Edition. New York : Informa Healthcare USA Inc.

Chaiyasut C, Bhagavathi SS, Sisithorn S, Netnapa M, Jakkapan S, Sartjin P,et al. 2018. Formulation and Stability Assessment of Arabica and Civet Coffee Extraccts Based Cosmetic Preparation. Asian Journal of Pharmaceutical and Clinical Research. June ;Vol 11:425-429.

Magnani C, Thais SO, Vera LI, Marcos AC, Herida RS. 2015. Validation of Caffeic Acid in Emulsion by UV-Spectrophotometric Method. Journal Physical Chemistry .5(1):16-22.

Chairgulprasert.V and Kittiya.K. 2017. Preliminary Phytochemical Screening and Antioxidant Activity of Robusta Coffee Blossom. Thammasat International Journal of Science and Technology. 22(1):1-8.

Tanuma HA, Gayatri C, Widya AL. 2016.Aktivitas antibakteri ekstrak biji kopi robusta (Coffea cenephora) terhadap bakteri Escherichia coli.Jurnal Ilmu Farmasi. 5(4):243-251.

Ciptaningsih,E. 2012. In Vitro Antioxidant Activity and Phtochemical Characteristics of Kopi Luwak and Its Effect on Blood Pressure in Normotensive and Hyoertensive Rats. Master of Pharmaceutical Sciences Thesis.Jakarta: University of Indonesia.

Sharma GK, Gadiya J, and Dhanawat M. 2016.Textbook of Cosmetic Formulation. India: Kbuuk Publication.

Safrida and Mustafa Sabri. 2017.Potential Of Aceh Arabica Coffee Extract (Coffea arabica L) in Rejuvenation of Aging Skin Rat.Jurnal Kedokteran Hewan.11(3):101-103.

Handayati D, Fifa A, Yuli K, Citra TM, Yudha DS. 2017.Efficacy of Topical Coffee Paste to Colagen Density in Wistar Rats Incision Wound. Bioscientia Medicina.2(1):12-15.

Grosselli B, Joseane M, Anglecio LS, Marilene NS, Amanda RA, Gislaine V, et al. 2017.Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus).In. Tatiana.M (Ed).PLOS.1-17.

Alam M and Havey J. 2010. Chapter 2: Photoaging . Cosmetic Dermatology: Products and Procedures. New York: Blackwell Publishing.

Putri E, Celvin A, Chrismis NG, Linda C, I Nyoman EL. 2019. Comparison of Anti-Aging Effectiveness from Gotu Kola Extract Cream (Centella asiatica) and Robusta Coffee Cream (Coffea canephora) Toward Hydration Levels in Male Mus Musculus Skin. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS).61(1):192-201.

Ribeiro H, Joana M, Sara R, Maria A, Vera I, Bruna GC, et al. 2012. From coffee industry waste materials to skin-friendly products with improved skin fat levels. Eur. J. Lipid Sci. Technol.115:330–336.

Fukagawa S, Satoshi H, Shun S,Yuka Y, Hisashi T, Takatoshi M. 2017. Coffe Polyphenols Extracted From Green Coffee Beans Improved Skin Properties and Microcirculatory Function. Biological Science Laboratories, Kao Corporation, Tochigi, Japan. Bioscience, Biotechnology, and Biochemistry Journal.1814-1822.

Magnani C, Thais SO, Vera LI, MarcosAC, Herida RS. 2015.Validation of Caffeic Acid in Emulsion by UV-Spectrophotometric Method. Journal Physical Chemistry .5(1):16-22.

Herman A, AP Herman. 2013.Caffeine’s Mechanisms of Action and its Cosmetic Use.Journal Skin Pharmacology and Physiology.Vol 26:8-14.




How to Cite

Girsang, L. C. ., Fachrial, E. ., & Lister, I. N. E. . (2020). Effectiveness Test of Robusta Coffee (Coffea cenephora) Extract from North Sumatra in Collagen and Hydration Skin Level of Female Wistar Rattus norvegicus. American Academic Scientific Research Journal for Engineering, Technology, and Sciences, 65(1), 108–115. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/5661