Identification and Characterization of the Antibacterial Activity of Fungal Metabolites on Contaminants Responsible for Foodborne Diseases
Keywords:
Bacterial resistance, Synergistic effect, Nigrospora, Aspergillus, PenicilliumAbstract
The emergence of new diseases due to recurrent food poisoning nowadays in the face of excessive use of conventional antibiotics leads to the search for new bioactive molecules. Thus, the objective of this work was to investigate the antibacterial activity of fungal metabolites on contaminants responsible for food poisoning. To do this, Eight (08) fungal strains belonging to the genera Aspergillus, Penicillium and Nigrospora were used and antibacterial tests were performed on 4 bacterial strains (Staphylococcus aureus ATCC 25923, Shigella sp, Escherichia coli sp and Salmonella sp) using the agar cylinder method (antibiotic). The results showed that seven (07) fungal isolates have high antibacterial activity with inhibition diameters ranging from 18 to 29 mm on Staphylococcus Aureus ATCC 25923; Shigella sp; Salmonella sp and Escherichia coli. Synergistic tests have shown that the combination of 4 to 5 fungal strains could increase bacterial inhibition of Staphylococcus, Salmonella, Shigella and Escherichia coli which appear resistant to the action of a single fungal strain.
References
Zoie Jones, Paul Garwood, Leticia Linn, S. O. and A. B. 2019. PAHO/WHO | Food safety is everyone’s business. Retrieved November 24, 2019, from PAHO website: https://www.paho.org/hq/index.php?option=com_content&view=article&id=15241:food-safety-is-everyone-s-business&Itemid=1926&lang=en
World Health Organization. 2019. New report calls for urgent action to avert antimicrobial resistance crisis. Retrieved November 24, 2019, from Joint News Release website: https://www.who.int/news-room/detail/29-04-2019-new-report-calls-for-urgent-action-to-avert-antimicrobial-resistance-crisis
Savin M.C.E. 2014. La Phagotherapie: Historique Et Potentielle Utilisation Contre Les Infections A Bacteries Multi Resistantes, These De Doctorat, Faculte De Medecine De Creteil. website: http://theses.vet-alfort.fr/telecharger.php?id=1445
Mehravar M. and Sardari S. 2011. Screening of Antimicrobial Membrane-Active Metabolites Of Soil Microfungi By Using Chromatic Phospholipid/Polydiacetylene Vesicles. Journal Of Medical Mycology, 21 (3:188-197.Website : https://www.researchgate.net/deref/http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.mycmed.2011.07.005
Pereira E., Santos A., Reis F., Tavares R.M., Baptista P., Lino-Neto T. And Almeidaaguiar C. 2013. A New Effective Assay To Detect Antimicrobial Activity Of Filamentous Fungi. Microbiological Research, 168 (1) : 1-5. https://doi.org/10.1016/j.micres.2012.06.008
Mansour, M., & Mazzi, Y. 2017. Recherche de l’activité antibactérienne des souches du genre Aspergillus Présenté (Université des Frères Mentouri Constantine). Retrieved from http://fac.umc.edu.dz/snv/faculte/biblio/mmf/2017/Recherche de l’activité antibactérienne des souches du genre Aspergillus.pdf
Bitsi Mjm. 2002. Isolement et caracterisation d’une Souche de champignon productrice d’antibiotiques. Diplôme D'études Approndies (DEA), University de Ouagadougou, Ouagadougou, P. 58.
LCSI. 2015. Performances standards for antimicrobial susceptibity testing M100S, 26 th Edition ; table 4A : Disk diffusion : Quality control ranges for nonfastidious. https://webstore.ansi.org/standards/clsi/clsim100s26
Mansour Mm, Al Hadidi Km, Hamed Ms. 2017. Epidemiology And Antimicrobial Resistance Of Community-Acquired Pneumonia In Children. J Health Res Rev, 4 : 122-9 DOI: 10.4103/jhrr.jhrr_75_15
Amadi, J. E. and Adeniyi, D. O. 2009. Mycotoxin Production By Fungi Isolated From Stored Grains Department Of Plant Biology, University Of Ilorin, P.M.B. 1515, Ilorin, Nigeria. Afr. J Of Biotechnology 8 (7) : 1219-1221. https://pdfs.semanticscholar.org/b773/927786760e6842108cb196afc4bb19e5f283.pdf
Nayak, B. K., & Anitha, K. 2014. Combined effects of antibiotics and AgNPs biosynthesized from Aspergillus ustus studied against few pathogenic bacteria. International Journal of PharmTech Research. https://www.researchgate.net/publication/288597078_Combined_effects_of_antibiotics_and_AgNPs_biosynthesized_from_Aspergillus_ustus_studied_against_few_pathogenic_bacteria
Gimenez C, Cabrera R, Reina M, Gonzalez-Coloma A. 2007. Fungal Endophytes And Their Role In Plant Protection. Curr. Org. Chem. 11 : 707-720. DOI: 10.2174/138527207780598765
Kumara C. G., Mongollaa P., Josepha J., Nageswara Y. V. D. And Kamal A. 2010. Antimicrobial Activity From The Extracts Of Fungal Isolates Of Soil And Dung Samples. From Kaziranga National Park, Assam, India. J Of Medical Mycology 2010, 20 : 283-289. https://doi.org/10.1016/j.mycmed.2010.08.002
Abdelaziz Wided (2006). Isolement Des Mycetes Producteurs De Substances Antibacteriennes A Partir Des Sols Sahariens. Mémoire De Magister En Microbiologie Et Biochimie Appliquees, Universite Mentouri De Constantine. https://bu.umc.edu.dz/theses/biologie/ABD4724.pdf
Tanaka, M., Fukushima, T., Tsujino, Y., & Fujimori, T. (1997). Nigrosporins A and B, New Phytotoxic and Antibacterial Metabolites Produced by a Fungus Nigrospora oryzae. Bioscience, Biotechnology, and Biochemistry, 61(11), 1848–1852. DOI: 10.1271/bbb.61.1848
Frisvad, J. C., Smedsgaard, J., Larsen, T. O., & Samson, R. A. (2004). Mycotoxins , drugs and other extrolites produced by species in Penicillium subgenus Penicillium. 201–241. http://www.wi.knaw.nl/publications/1049/part6.pdf
Houbraken J, M Due, J Varga, M Meijer, Jens Christian Frisvad, Robert A Samson (2007). Polyphasic Taxonomy Of Aspergillus Section Usti 2007 Sudies In Mycology 59, 107-128. doi: 10.3114/sim.2007.59.12
Raistrick, H., & Stickings, C. E. (1951). Studies in the biochemistry of micro-organisms; ustic acid, a metabolic product of Aspergillus ustus (Bainier) Thom & Church. The Biochemical Journal, 48(1), 53–66. https://doi.org/10.1042/bj0480053.
Rathod DP, Dar MA, Gade AK and Rai MK. 2014. Grisofulvin Producing Endophyting Nigrospora Oryzae From Indian Emblica Officinalis Gaertn : A new report. Austin Journal Of Biotechnology & Bioengineering Volume 1, 3. https://austinpublishinggroup.com/biotechnology-bioengineering/fulltext/download.php?file=ajbtbe-v1-id1015.pdf
Thennarasu. V., Thajuddin, N. & Panneerselvam, A. 2015. Antibacterial Activity Of Penicillium Spinulosum Ttmf2 Isolated From Marine Soils of Chidiya Tapu In Andaman And Nicobar Islands.2015 World Journal Of Pharmaceutical Research 4, 2577- 2583. https://wjpr.net/admin/assets/article_issue/1430825684.pdf
Aouarib, K. and Lemsara, R. (2016). Étude Des Activites Antimicrobienne Et Enzymatique Des Champignons Endophytes Isoles A Partir D’artemisia Absinthium 2016: 28-50. http://www.secheresse.info/spip.php?article57279
Vega, N. M., & Gore, J. (2014). Collective antibiotic resistance: Mechanisms and implications. Current Opinion in Microbiology 21, 28–34. https://doi.org/10.1016/j.mib.2014.09.003.
Downloads
Published
How to Cite
Issue
Section
License
Authors who submit papers with this journal agree to the following terms.