Comparison of Antioxidant and Anti-collagenase Activities Ethanol Extract of Black Soybeans with Daidzein Compounds

Authors

  • Lubna Alqodri Faculty of Medicine, Universitas Prima Indonesia, Medan, Sumatera Utara, Indonesia
  • Ermi Girsang Faculty of Medicine, Universitas Prima Indonesia, Medan, Sumatera Utara, Indonesia
  • Ali Napiah Nasution Faculty of Medicine, Universitas Prima Indonesia, Medan, Sumatera Utara, Indonesia
  • Sahna Ferdinand Ginting Faculty of Medicine, Universitas Prima Indonesia, Medan, Sumatera Utara, Indonesia

Keywords:

Antioxidant, Anti-collagenase, Black Soybean, Daidzein

Abstract

Aging is a natural process in human life; one of the triggers is free radicals. The use of antioxidants from natural ingredients is an effort to overcome premature aging and counteract the harmful effects caused by free radicals. Black soybean (Glycine soja), which easily obtained in Indonesia, contains a nutritional value that is very good for human health. The protein content is quite high (40.4 g / 100g) and rich in antioxidants. The results presented in this study revealed that black soybean extract (Glycine max (L.) Merr.) Had a higher antioxidant activity of FRAP compared to Daidzein compounds, black soybean extract had antiaging activity through better collagenase inhibition compared to Daidzein compounds. The largest FRAP activity at 50 ?g / mL black soybean extract concentration was 146.35 ?M Fe (II) / ?g) and Daidzein 50 ?g / mL concentration was 96.93 (?M Fe (II) / ?g). Anticolagenase activity based on the IC50 value of black soybean extract was 84.73 ?g / mL, and Daidzein was 98.18 ?g / mL. From the above results, it can conclude that ethanol extract of black soybeans has excellent potential as an antioxidant that is useful as inhibiting collagenase.

References

. Anderson,J.W. 2002. Meta-analysis of the Effect of Soy Proteins Intake of Serum Lipid. J.Med. 333: 276-282.

. Arct J, Pytkowska K. Flavonoids of biologically active cosmeceuticals. Clin Dermatol. 2008(26): 347–357.

. Bao, C., Namgung, H., Lee, J., Park, H.C., Ko, J., Moon, H., Ko, H.W., Lee, H.J. (2014). Daidzein suppresses tumor necrosis factor-α induced migration and invasion by inhibiting hedgehog/Gli1 signaling in human breast cancer cells. J. Agric. Food. Chem. 30:3759-3767.

. Benaiges,A.,P.Marcet.,R.Armengol.,C.Betes.,E.Girones. 1998. Study of the refirming effect of a plant complex. Int J Cosmet Sci. 1998(20): 223–233.

. Charkoudian, N. (2003). Skin blood flow in adult human thermo-regulation: How it works, when it works, when it does not, and why. Mayo Clinic Proceedings, 78(5), 603-612

. Choi, E.J., Kim, G.H. (2013). Antiproliferative activity of daidzein and genistein may be related to ERα/c-erbB-2 expression in human breast cancer cells. Mol. Med. Rep. 7: 781-784.

. Chu, D. H. (2008). Overview of biology, development, and structure of skin. In K. Wolff, L. A. Goldsmith, S. I. Katz, B. A. Gilchrest, A. S. Paller, & D. J. Leffell (Eds.), Fitzpatrick's dermatology in general medicine (7th ed., pp. 57-73). New York: McGraw-Hill.

. Cotsarelis, G., Sun, T. T., & Lavker, R. M. (1990). Label-retaining cells reside in the bulge of the pilosebaceous unit: Implications for follicular stem cells, hair cycle, and skin carcinogenesis. Cell, 61(7), 1329-1337.

. Coward L, Barnes NC, Serchell KD, Barnes S. 1993. Genistein, Daidzein, and their betha-glycosidase conjugates: antitumor isoflavone in soybean foods from American and asian diets. J Agric Food Chem 41:1961–1967.

. Dajanta, K., Janpum, P. & Leksing, W. 2013. Antioxidant Capacities, Total Phenolics and Flavonoids in Black and Yellow Soybeans Fermented by Bacillus subtilis: A Comparative Study of Thai Fermented Soybeans (thuanao). International Food Research Journal. Vol. 20 (6): 3125-3132.

. Danby, F. W. (2005). Why we have sebaceous glands. Journal of the American Academy of Dermatology, 52(6), 1071-1072.

. Daniel, R. C., & Scher, R. K. (1997). Nail changes secondary to systemic drugs and ingestants. In R. K. Scher, & R. C. Daniel (Eds.), Nails: Therapy, diagnosis, surgery (2nd ed., pp. 251-258). Philadelphia: Saunders.

. Danilenko, D. M., Ring, B. D., & Pierce, G. F. (1996). Growth factors and cytokines in hair follicle development and cycling: Recent insights from animal models and the potentials for clinical therapy. Molecular Medicine Today, 2(11), 460-467.

. Dubey, R.K., Imthurn, B., Zacharia, L.C., Jackson, E.K. (2004). Hormone replacement therapy and cardiovascular disease what went wrong and where do we go from here? Hypertension. 44: 789-795.

. Ernita E. 1995. Senyawa-senyawa Isoflavon dari Limbah Tahu [skripsi]. Bogor: Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor.

. Fairley, J. A., Scott, G. A., Jensen, K. D., Goldsmith, L. A., & Diaz, L. A. (1991). Characterization of keratocalmin, a calmodulin-binding protein from human epidermis. Journal of Clinical Investigation, 88(1), 315-322.

. Farnsworth, N. R. 1966. Biological and Phytochemical Screening of Plants. J Pharm Sci ; 55(3): 243–264.

. Fawwaz,M.,D.S.Muliadi.,A.Muflihunna. 2018. Kedelai hitam (Glycine soja) Terhidrolisis Sebagai Sumber Flavonoid Total. Jurnal Fitofarmaka Indonesia-Research Gate. 4(1): 194-198.

. Fisher,G.,S.Kang.,J.Varani.,Z.Bata-Csorgo.,Y.Wan.,S.Datta.,J.J.Voorhees. 2002. Mechanisms of Photoaging and Chronological Skin Aging. Arch Dermatol. 138: 1462-1470.

. Fisher,A.E.O.,T.A.Hague.,C.L.Clarke.,D.P.Naughton. 2004. Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG. Biochem Biophys Res Commun. 2004(323): 163–167.

. Flaxman, B. A., Sosis, A. C., & Van Scott, E. G. (1973). Changes in melanosome distribution in Caucasoid skin following topical application of nitrogen mustard. Journal of Investigative Dermatology, 60(5), 321-326.

. Franke AA, Custer LJ, Cerna CM, Narala KK. 1994. Quantitation of phytoestrogens in legumes by HPLC. J Agric Food Chem 42:1905–1913.

. Friedman M, Brandon DL. 2001. Nutritional and helth benefits of soy protein. J Agric Food Chem 49:1069–1086.

. Gayraud, B., Hopfner, B., Jassim, A., Aumailley, M., & Bruckner-Tuderman, L. (1997). Characterization of a 50-kDa component of epithelial basement membranes using GDA-J/F3 monoclonal antibody. Journal of Biological Chemistry, 272(14), 9531-9538.

. Genovesse MI, Davila J, Lajolo FM. 2006. Isoflavones in processed soybean product from Ecuador. Braz Arch Biol Tech 49(5):853–859Haake, A. R., & Hollbrook, K. (1999). The structure and development of skin. In I. Freedberg, A. Eisen, K. Wolff, K. Austen, L. Goldsmith, S. Katz, et al. (Eds.), Fitzpatrick's dermatology in general medicine (5th ed., pp. 70-111). New York: McGraw-Hill.

. Jenkins, D. J., C. W Kendall, L. S. Augustin et al. 2002. Glycemic index: overview of implications in health and disease. Am. J. Clin. Nutr. 76:266S-273S.

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Published

2020-06-26

How to Cite

Alqodri, L. ., Girsang, E. ., Nasution, A. N. ., & Ginting, S. F. . (2020). Comparison of Antioxidant and Anti-collagenase Activities Ethanol Extract of Black Soybeans with Daidzein Compounds. American Scientific Research Journal for Engineering, Technology, and Sciences, 70(1), 90–98. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/5560

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Vol. 70 No. 1 (2020)

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