Antioxidant and Tyrosinase Enzyme Inhibition Activity of Lime Peel and Seed Ethanol Extract

  • Leo Mirah Faculty of Medicine, University Prima Indonesia, Medan, North Sumatera, Indonesia
  • Ermi Girsang Faculty of Public Health, University Prima Indonesia, Medan, North Sumatera, Indonesia
  • Ali Napiah Nasution Faculty of Medicine, University Prima Indonesia, Medan, North Sumatera, Indonesia
  • I Nyoman Ehrich Lister Faculty of Medicine, University Prima Indonesia, Medan, North Sumatera, Indonesia
Keywords: Lime, Peel, Seed, ABTS, Tyrosinase enzyme

Abstract

UV Light exposure and Oxidative stress can cause the accumulation of free radicals and increase melanogenesis. The various herbal plant enriches Indonesia. One of them is lime that content various phytochemicals. This study was aimed to explore antioxidants property and tyrosinase enzyme inhibition activity of lime peel and lime seed. This study used ethanol extract from lime peel and seed. The antioxidant activity was evaluated by the ABTS reduction assay, while tyrosinase enzyme inhibitor activity by tyrosinase enzyme from fungi. The results of this study showed that the IC50 Value of Lime Seed (14.09 ± 0.88 µg/mL) was lower than lime peel (20.16 ± 0.23 µg/mL). Meanwhile, the IC50 value against tyrosine enzyme of Lime Peel (34.23 ± 0.93 µg/mL) was lower than lime seed (67.88 ± 3.73µg/mL). Hence lime peels potentially become a tyrosinase enzyme inhibitor agent while the lime seed has better antioxidant potential than the peel.

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Published
2020-01-22
Section
Articles