Analysis of Protein Content, Spectrophotometry FT-IR, and Antibacterial Effects of Earthworm (Eudriluseugenia)

  • Suandy Master in Biomedical Sciences Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera
  • Ermi Girsang Faculty of Public Health, Universitas Prima Indonesia, Medan, North Sumatera
  • Ali Napiah Nasution Master in Biomedical Sciences Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera
  • I Nyoman Ehrich Lister Master in Biomedical Sciences Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera
Keywords: Eudrilus eugeniae, coelomic fluid, Ciprofloxacin, Staphylococcus aureus, Escherichia coli

Abstract

This research to compare effectiveness of coelomic fluid and extract ethanol of Eudriluseugeniae against Staphylococcus aureus and Escherichia coli as antibacterial. Suggest that protein on Eudriluseugeniae have antibacterial activity.Experimental laboratory with analytical statistic Mann- Whitney with following step of research: (1) Determination of earthworm, (2) Cold shock methods modification to get coelomic fluid, (3) Maceration ethanol 70 % of Eudriluseugeniae.(4) Determination protein content with Kjehdahl methods, (5) Identification absorbance infrared by spectrophotometry FT-IR .(7) Antibacterial assay using disc diffusion against Staphylococcus aureus and Escherichia coli.The result of this study showed that protein content of coelomic fluid (1.875 %)were higher than ethanol extract (0.437%) of Eudrilus eugenia. Coelomic liquid had no functional group in the chemical compound. However, the ethanol extract showed some functional group includes aliphatic, ethanol, and Amide A group. The antibacterial effect against Escherichia coli and Staphylococcus aureus from ethanol extract or coelomic liquid of Eudriluseugeniae were similar at equal concentration. It due to p value of analysis was lower than 0.05.Coelomic liquid and ethanol extract had similar antibacterial effects against Staphylococcus aureus and Escherichia coli.

References

N. K. S. S. Ningsih dan T. Setyawati, “Perbandingan Efektivitas Antibiotik (Ciprofloxacin, Cefotaximen, Ampicilin, Ceftazidime, dan Meropenem) terhadap Bakteri Staphylococcus auerues Penyebab Ulkus Diabetik Metode Kiirby-Bauer,” J. Ilm. Kedokt., vol. 3, no. 2, hal. 1–11, 2016.

B. Chudlori, M. Kuswandi, dan P. Indrayudha, “Pola kuman dan resistensinya terhadap antibiotika dari spesimen pus di RSUD Dr. Moewardi,” J. Farm. Indones., vol. 13, no. 2, hal. 70–76, 2012.

M. F. AL-Marjani, K. A. Kadhim, A. A. Kadhim, dan A. A. Y. Kinani, “Ciprofloxacin resistance in Staphylococcus aureus and Pseudomonas aeruginosa isolated from patients in Baghdad,” Int J Pharm Sci Res, vol. 6, no. 2, hal. 382–385, 2015.

G. Ayundini dan N. P. Adi, “Penggunaan Antibiotik Topikal sebagai Alternatif Terapi Ulkus Kaki Diabetik,” eJournal Kedokt. Indones., vol. 2, no. 2, hal. 7–10, 2014.

E. L. Cooper et al., “Earthworms dilong: Ancient, inexpensive, noncontroversial models may help clarify approaches to integrated medicine emphasizing neuroimmune systems,” Evidence-based Complement. Altern. Med., vol. 2012, hal. 1–11, 2012.

J. A. Kanis, E. V. McCloskey, H. Johansson, C. Cooper, R. Rizzoli, dan J. Y. Reginster, “European guidance for the diagnosis and management of osteoporosis in postmenopausal women,” Osteoporos. Int., 2013.

Z. Sun, “Earthworm as a biopharmaceutical: from traditional to precise,” Eur. J. Biomed. Res., vol. 1, no. 2, hal. 28–35, 2015.

N. Mathur, G. C. Jain, dan G. Pandey, “Anti-Inflammatory Activity of Earthworm Extracts,” Int. J. Pharm. Sci. Res., vol. 2, no. 2, hal. 278–281, 2011.

C. Esaivani, K. Vasanthi, dan A. J. . R. Singh, “An Investigation on Antimicrobial Potency of Coelomic Fluid of Earthworm Eudrilus eugeniae,” Br. J. Med. Heal. Res., vol. 4, no. 4, hal. 18–27, 2017.

P. S. Chauhan, J. Tomar, G. B. K. S. Prasad, dan O. P. Agrawal, “Evaluation of antimicrobial activity if earthworm tissue extract of Eudrilus eugeniae,” J. Chem. Pharm. Res., vol. 6, no. 8, hal. 28–38, 2014.

S. Reddy dan R. Kale, “Antimicrobial potency of earthworm, Eudrilus eugeniae on certain plant pathogens,” Jan 2007.

J. Waluyo, B. Sugiharto, dan C. Zaini, “Purifikasi dan Karakterisasi Protein Antibakteri dari Pheretima javanica ( Purification and Characterization of Antibacterial Protein from Pheretima javanica ),” J. Ilmu Dasar, vol. 8, no. 1, hal. 37–44, 2007.

H. Rosaini, R. Rasyid, dan V. Hagramida, “Penetapan Kadar Protein Secara Kjehdahl Beberapa Makanan Olahan Kerang Remis (Corbiculla moltkiana Prime) dari Danau Singkarak,” J. Farm. Higea, vol. 7, no. 2, hal. 120–127, 2015.

Published
2020-01-22
Section
Articles