Effectivity of Gel Ethanolic Extract of Senggani Leaves (Melastoma candidum D. Don) In Increasing The Number of Fibroblast Cells and Thickness of Collagen Fibers Against Socket Wound after Tooth Extraction on Male White Rats
Keywords:
Gel ethanolic extract of Senggani Leaves, Rattus norvegicus, number of fibroblast Cell, Collagen density score, tooth extraction and wound healingAbstract
The important cells in wound healing process are the fibroblast cells and the density of collagen fibers. The Senggani leaves (Melastoma candidum D.Don) contains some active compound of flavonoids / phenols, triterpenoid / steroid, saponins, tannins and glycosides. This research aims to determine the effectiveness of gel ethanolic of Senggani leaves (Melastoma candidum D.Don) towards increased number of fibroblast cells and collagen density score in the process of wound healing after tooth extraction on male white rats (Rattus noevegicus). The research design was purely experimental in vivo, design post test only control group and non – randomly or non – probability sampling using the sample of 25 Wistar rats. Male white rats anesthetized intra-peritoneal (IP) using ketamine 100 mg / 1 ml at a dose of 20-40 mg/kg body weight of the rat, then extraction of the mandibular left incisor using a sharp excavator and pulling the pliers off, then Dental sockets are irrigated with a sterile was given Aloclair Plus gel ®, the treatment group was given 1% EDS, 5% EDS and 10% EDS twice a day with a frequency of 12 times the treatment.
On the seventh day, euthanasia was carried out, the rats were put into a closed box containing ether cotton until suffocation, followed by histological tissue making. Histology preparations were seen with the Boeco Binocular microscope BM-180 SP model, with the help of MDCE-5A digital microscope camera connected to a binocular microscope through the eyepiece entry hole, and using a 10 x objective lens and analyzed using Adobe Photoshop CS 6.0 software to view a number of fibroblast cells and density of collagen fibers. Calculations were carried out with 5 fields of view then calculated how many fibroblasts and collagen density scores in each field of view so that they were clearly visible. From the field of view 1 to 5 it is added up, taken the average. The results of descriptive analysis of the number of fibroblast cells and collagen fiber density scores showed that the average number of fibroblast cells and the highest collagen fiber density score in the 10% EDS treatment group were 83,133 and 3,933, followed by the positive control group of 82,667 and 3,667, the 5% EDS treatment group is 41,200 and 3,067, the EDS treatment group is 1% which is 33,800 and 3,000, and the lowest result is negative control which is 16,533 and 1,200. Data analysis was using Shapiro Wilk normality test, Levene homogeneity test, One way Anova test and the test of the comparative effect (LSD = Least Significant Difference and Tukey's HSD = Honestly Significant Difference). The results showed that has normal distribution of data (p > 0,05) and homogeneity of data (p> 0,05). One way annova test results the significant number of fibroblast cells and collagen fiber density score on the fifth group (given Melastoma candidum D. Don rind extract gel concentration of 10 %). The conclusion showed that Melastoma candidum D. Don rind extract gel concentration of 10 % is effective towards increased the number of fibroblast cells and thickness of collagen fibers score against socket wounds after tooth extraction in male white rats.
References
. Nadia Farhatika, 2018.Efektifitas Gel Ekstrak Biji Kakao (Theoborma cacao L.) Terhadap Intensitas Kolagen Pada Penyembuhan Lika Jaringan Lunak Soket Pasca Pencabutan Gigi Tikus Wistar. Fakultas Kedokteran Gigi, Universitas Jember.
. Oroh CG, Pangemanan DHC, Mintjelungan CN. Efektivitas lendir cekicot (Achatina fulica) terhadap jumlah sel fibroblas pada luka pasca pencabutan gigi tikus Wistar. J E-Gigi. 2015;3(2):515–20.
. Nyoman Sidi Wisesa, 2017. Kombinasi Pasta Ekstrak Daun Jambu Biji (Psidium guajava linn.) 5% dan Povidon Iodine 10% Efektif Meningkatkan Jumlah Fibroblas dan Ketebalan Kolagen Pasca Pencabutan Gigi Marmut (Cavia cobaya), Program Magister. Program Studi Ilmu Biomedik, Fakultas Kedokteran Universitas Udayana, Denpasar.
. Sjamsuhidajat R., Warko K., Theddeus OHP, Reno B. Buku Ajar Ilmu Bedah (3 rd ed.) Jakarta: EGC. 2012. 95-101.
. Rahayu, Fitri., Wiwit Ade FW., Wiwik Rahayu. 2013. Pengaruh Pemberian Topikal Gel Lidah Buaya (Aloe cinensis Baker) Terhadap Reepitelisasi Epidermis pada Luka Sayat Kulit Mencit (Mus musculus).Universitas Riau. Pekanbaru.
. ITD Kusumowati, Rosita Melannisa, Angga Prasetyawan. Daya Antibakteri Ekstrak Etanol Daun Senggani (Melastoma Affine D.Don) Fakultas Farmasi Universitas Muhammadiyah Surakarta, Agustus 2014: Vol 6(2): 20-25
. Olivia Avriyanti Hanafiah, 2019. Perbedaan Ekspresi TGF B1 dan PDGF – BB sebagai Penanda Proliferasi Sel Fibroblas dengan Pemberian Gel Ekstrak Daun Benahong (Anredera cordifolia (Ten.) Steenis) dan Gel Aloclair R Pada Penyembuhan Luka di Mukosa Palatal Tikus Wistar, Program Doktor (S-3) Ilmu Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Sumatera Utara, Medan.
. Andi Irdam Hidayat. Uji Aktifitas Antimikroba Fraksi Ekstrak Daun Senggani (Melastoma affine D.Don) Terhadap Mikroba Patogen. Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Alauddin. 2017; 7-9.
. Backer, C.A. and Bakhuizen V.d. Brink, Jr. 1968. Flora of Java Volume 3. Netherlands: Netherlands Organisation for the Advancement of Research
. Sentra Informasi IPTEK, 2009. Senggani (Melastoma Candidum D. Don) http://www.iptek.net.id/ind/pd_tanobat/view.php/mnv=2&id=156 [ 09April 2009]
. Robertus Wandi, 2015. Efektifitas Gel Combustio Derajat II Ekstrak Etanol Daun Senggani (Melastoma malabathricum L.) Pada Tikus Jantan (Rattus nurvegicus), Program Studi Farmasi, Fakultas Kedokteran Universitas Tanjung Pura, Pontianak.
. Sry Wardiyah, 2015. Perbandingan Sifat Fisik Sediaan Krim,Gel, dab Salep yang Mengandung Etil P – Metoksisinamat dari Ekstrak Rimpang Kencur (Kaempferia galanga Linn.). Fakultas Kedokteran dan Ilme Kesehatan, Program Studi Farmasi. UIN Syarif Hidayatullah, Jakarta.
. Ulfa Zara Izzati, 2015. Efektifitas Penyembuhan Luka Bakar Salep Ekstrak Etanol Daun Senggani (Melastoma malabathricum L.) Pada Tikus (Rattus norvegicus) Jantan Galur Wistar. Program Studi Farmasi Fakultas Kedokteran, Universitas Tanjungpura, Pontianak.
. Mappa T, Edy, J.H., Kojong, N. Formulasi Gel Ekstrak Daun Sasaladahan (Peperomia pellucida (L.) H.B.K) dan Uji Efektifitasnya Terhadap Luka Bakar pada Kelinci (Oryctolagus cuniculus). Jurnal Ilmiah Farmasi. 2013; 2(2); Hal 49-55.
. Zulfah Al-Fa’izah, 2018. Efektifitas Gel Ekstrak Biji Kakao(Theobroma cacao L.) Terhadap Jumlah Sel Fibroblas Pada Soket Pasca Pencabutan Gigi Tikus Wistar Jantan. Fakultas Kedokteran Gigi, Universitas Jember.
. Soekidjo Notoatmodjo, S.K.M., M.Com.H., 2018, Metodologi Penelitian Kesehatan, Jakarta. Rhineka Cipta. Cetakan ketiga.
. Hafidhah, N., R.F.Hakim, dan Fakhurranzi, 2017. Pengaruh ekstrak biji kakao (Theobroma cacao L.) terhadap penumbuhan Enterococcus faecalis pada berbagai konsentrasi. Journal Canalis Dentistry. 2(2): 341 -343.
. Isye Matiani, et.al., 2013. Laporan Praktikum Farmakognosi Penetapan Kadar Sari Dalam Pelarut Tertentu, Jurusan Farmasi Fakulktas Matematika dan Ilmu Pengetahuan Alam, Universitas Garut.
. Fuadi, M.I., E. Ulfa dan Misnawi, 2015. Jumlah Fibroblas Pada Luka Bakar Derajat II pada Tikus dengan Pemberian Gel Ekstrak Etanol Biji Kakao dan Silver Sulfadiazine. E- Jurnal Pustaka Kesehatan. 3(2): 247-248.
. Barua, N., Absar, N., Paul, S., Barua, A., Gazi, M.Y., Saha, M., Islam, M.S., Belaly, J.M., 2014. In Vitro Phytochemical, Cytoxicity and Mineral Composition Analyses of Micania cordata (Bumr.f.) B.L. Robinson Leaves. International Journal of Biosciences. 5(8): 154-160.
. Dewi IALP, Damriyasa IM, Dada IKA. Bioaktivitas Ekstrak Daun Tapak Dara (Catharanthus roseus) terhadap periode epitelisasi dalam proses penyembuhan luka pada tikus wistar. Indonesia Medicus Veterinus 2013; 2(1): 58-75.
. Ferdinandez MK, Dada IKA, Damriyasa IM. Bioaktivitas Ekstrak Daun Tapak Dara (Catharantus roseus) Terhadap Kecepatan Angiogenesis Dalam Proses Penyembuhan Luka Pada Tikus Wistar. Indonesia Medicus Veterinus 2013; 2(2): 180-190.
. Nurcahaya MI. Pengaruh ekstrak etanol lidah buaya (Aloe vera) terhadap peningkatan jumlah fibroblas pada proses penyembuhan luka mukosa rongga mulut tikus (Rattus norvegicus) strain wistar.Skripsi. Surakarta: 2015. P. 35.
. Guy Masir O, Manjas M,Putra AE, Agus S. Pengaruh cairan Cultur Filtrate Fibroblast (CFF) terhadap penyembuhan luka: penelitian eksperimental pada Rattus novergicus galur Wistar. Jurnal Kesehatan Andalas. 2012; 1(3): 112-117.
. Glim JE, Egmond MV, Niessen FB,Everts V, Beelen RH. Detrimental dermal wound healing: What can we learn from the oral mucosa. International Jounal of tissue repair and regeration. 2013; 21(5): 648-660.
. Guo S. Dipietro LA. Factors Affecting Wound Healing. Journal of Dental Research. 2010; 89(3): 219-229.
. Orsted HL, Keast D, Lalande LF, Megie MF. Basic Principles of Wound Healing: An Understanding of the basic physiology of wound healing provides the clinician with the framework necessary to implement the basic principles of chronic wound care. Wound Care Canada 2011, 9(2): 4-12.
. Velnar T, T Bailey, V Smrkolj. The wound healing process: an overview of the cellular and molecular mechanism. The Journal of International Medical Research 2009; 37(5): 1528-1542.
. Suryadi IA, Asmarajaya AAGN, Maliawan S. Proses penyembuhan dan penanganan luka. Denpasar. P3-4.
. Triyono B. Program perbedaan tampilan kolagen di sekitar luka insisi pada tikus wistar yang diberi infiltrasi penghilang nyeri levobupivakain dan yang tidak diberi levobupivakain. Tesis 2005. p. 25
. Chairunnisa A. Pengaruh aplikasi ekstrak daun ceremai (Phyllanthus acidus (L.)) terhadap jumlah fibroblas pada hari ke-7. Skripsi. Banda Aceh: 2015. P. 29.
. Nayak BS, Pereira LMP. Catharantus roseus flower extracthas wound-healing activity in Sprague dawley rats. BMC Complementary and Alternative Medicine 2006; 6(41): 1-6.
. Fitriyani A, Winarti L, Muslichah S dan Nuri. Uji antiinflamasi ekstrak metanol daun sirih merah (Piper crocatum Ruiz & Pav) pada tikus putih. Majalah Obat tradisional 2011; 16(1): 34-42.
. Hidayati NA, Listyawati S, Setyawan AD. Kandungan kimia dan uji antiinflamasi ekstrak etanol Lantara camara L. Pada tikus putih (Rattus novergicus L.) jantan. Bioteknologi 2008; 5(1): 10-17.
. Priosoeryanto B, Huminto H, Wientarsih I, Estuningsih S. Aktivitas Getah Batang Pohon Pisang dalam Proses Persembuhan Luka dan Efek Kosmetiknya pada Hewan. Lembaga Penelitian dan Pemberdayaan Masyarakat. Institut Pertanian Bogor, 2006: 11(2): 70-73.
. Harisaranraj R, Suresh K, Saravanababu S. Evaluation of the Chemical Composition Rauwolfia serpentine and Ephedra vulgaris. Advances in Biological Research, 2009; 3(5): 174-178.
. Hasanoglu. Efficacy of Micronized Flavonoid Fraction in Healing of Clean and Infected Wounds. Medicine Oral, 2001; 10 (1): 41-44.
. Dian NI. Isolasi dan elusidasi struktur senyawa turunan terpenoid dari kulit batang slatri (Calophyllum soulattri Burm f.) Skripsi. 2011. p. 13-14.
. Simanjuntak MR. Ekstraksi dan fraksinasi komponen ekstrak daun tumbuhan senduduk (Melastoma malabathricum L.) serta pengujian efek sediaan krim terhadap penyembuhan luka bakar. Skripsi. Medam: 2008. p.6.
Downloads
Published
How to Cite
Issue
Section
License
Authors who submit papers with this journal agree to the following terms.
