Protective Effect of Curcuma zedoria Against Copper in Rats’ White Blood Cell


  • Linda Chiuman Prima Indonesia University


Curcuma zedoria, copper, CuSO4, curcumin, WBC


Copper, an essential trace element, is essential for the human body to maintain homeostatic balance. Higher levels of copper in the human body can cause damage to the liver, kidney, and brain due to increased generation of ROS (reactive oxygen species). As an alternative solution, natural resources which can be used to reduce the impact of copper excess is Curcuma zedoria due to the presence of curcumin. This study was aimed to explore the protective effect of Curcuma zedoria against copper in rats using the WBC as an indicator. This study was performed using 15 rats that were divided into 5 groups consisting of a negative group, a positive group, and three experimental groups which were given ethanol extract of Curcuma zedoria in various dosage. Each group of rats was fed 0.36 mg/kg b.w of CuSO4 solution on the 10th to 14th day of the experiment. The results showed that the level of band neutrophil, lymphocyte, and monocyte increased following the reduction of the dosage of Curcuma Zedoria that given to the rats. However, the levels of monocyte in each group were within normal range except the negative group. Based on the higher levels of WBC, all of the experiment groups suffered chronic active inflammation. Curcuma zedoria had a protective effect against overexposure to copper in rats, but it didn’t completely protect the rats from the effects of overexposure.


L. Wilson, Toxic Metals and Human Health. Mexico: Eck Institute Nutrition and Bioenergetics, 2012.

M. A. Septiono and D. Roosmini, ‘Heavy Metal Distribution in Water, Sediment, and Fish at Upper Citarum River and Its Potential Exposure Pathway to Human’, in Environmental Technology & Management Conference, 2015, pp. 1–10.

M. Bost, S. Houdart, M. Oberli, E. Kalonji, J. F. Huneau, and I. Margaritis, ‘Dietary copper and human health: Current evidence and unresolved issues’, J. Trace Elem. Med. Biol., vol. 35, pp. 107–115, 2016.

L. S. P. Bugata et al., ‘Acute and subacute oral toxicity of copper oxide nanoparticles in female albino Wistar rats’, J. Appl. Toxicol., vol. 39, no. 5, p. 2, 2019.

Y. Rivera-Espinoza and P. Muriel, ‘Pharmacological actions of curcumin’, John Wiley Sons. Ltd, vol. 29, no. 10, pp. 1457–1466, 2009.

S. Lakshmi, G. Padmaja, and P. Remani, ‘ Antitumour Effects of Isocurcumenol Isolated from Curcuma zedoaria Rhizomes on Human and Murine Cancer Cells ’, Int. J. Med. Chem., 2011.

T. C. B. Pereira, M. M. Campos, and M. R. Bogo, ‘Copper toxicology, oxidative stress and inflammation using zebrafish as experimental model’, J. Appl. Toxicol., 2016.

H. Mohan, Textbook of Pathology Sixth Edition. New Delhi: Jaypee, 2010.

V. Kumar, R. S. Cotran, and S. L. Robbins, Buku Ajar Patologi Robbins, 9th ed. Jakarta: EGC, 2015.

A. L. Meschel, Histologi Dasar Junqueira Teks & Atlas, 12th ed. Jakarta: EGC, 2017.

M. H. Ross and W. Pawlina, Histology a Text and Atlas. Philadelhia: Wolters Kluwer, 2011.

B. F. Rodak and J. H. Carr, Clinical Hematology Atlas Fifth Edition. China: Elsevier, 2017.

N. X. Ongko, L. Chiuman, and C. N. Ginting, ‘Effect of White Turmeric Rhizome Extract (Curcuma zedoaria) on Testis Histology of Male Wistar Rat’, Am. Sci. Res. J. Eng. Technol. Sci., vol. 55, no. 1, pp. 69–74, 2019.




How to Cite

Chiuman, L. . (2019). Protective Effect of Curcuma zedoria Against Copper in Rats’ White Blood Cell. American Scientific Research Journal for Engineering, Technology, and Sciences, 59(1), 142–148. Retrieved from