Bacteriological Evaluation of Drinking Water of Rajshahi City, Bangladesh

Authors

  • Md Fazlul Haque Genetics and Molecular Biology Laboratory, Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh
  • Sathi Rani Sarker Genetics and Molecular Biology Laboratory, Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh
  • Ananda Kumar Saha Genetics and Molecular Biology Laboratory, Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh
  • Moni Krishno Mohanta Genetics and Molecular Biology Laboratory, Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh
  • Arnaba Saha Chaity Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh
  • Tamanna Nasrin Genetics and Molecular Biology Laboratory, Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh
  • Rokshana Ara Ruhi Genetics and Molecular Biology Laboratory, Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh
  • Sk. Md. Atiqur Rahman Genetics and Molecular Biology Laboratory, Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh

Keywords:

Bacteria, Pollution, drinking water, Rajshahi

Abstract

Access to pathogen free safe drinking water is one of the major challenges of the 21st century for most of the people of world. According to WHO guidelines, 100 ml sample of safe drinking must be free of total coliform, fecal coliform and E. coli. Hence, this study was designed for bacteriological evaluation of drinking water collected from different restaurant in Rajshahi city, Bangladesh. Among 10 samples, no detectable E. coli was found in 5 water samples (100 ml each) while 8-50 detectable E. coli in 100 ml water sample was found in the remaining 5 samples which exceeded the BDS and WHO standard of drinking water. Four randomly selected isolates (B1, B2, B3 and R) which were isolated from different water samples were subjected to 16S rRNA gene sequencing. It was found  that the 16S rRNA sequence of isolate B1, B2 and B3 had 98% similarity to that of Enterobacter asburiae, Escherichia coli and Acinetobacter sp respectively. Similarly, 16S rRNA sequence of isolate R had 99% similarity to that of Klebsiella pneumonia. Results of biochemical analysis revealed that all four isolates were negative to Catalase, KOH and Oxidase test while variations were found for other tests. Similarly, all four isolates were able to ferment all tested carbohydrates except cellulose. The optimum pH and temperature for the growth of the all isolates was pH 7.0 and 37ºC. It was found that all the isolates were sensitive to majority of the antibiotics used.

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Published

2019-09-22

How to Cite

Haque, M. F., Rani Sarker, S. ., Kumar Saha, A. ., Krishno Mohanta, M. ., Saha Chaity, A. ., Nasrin, T. ., Ara Ruhi, R. ., & Rahman, S. M. A. . (2019). Bacteriological Evaluation of Drinking Water of Rajshahi City, Bangladesh. American Scientific Research Journal for Engineering, Technology, and Sciences, 59(1), 230–243. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/4981

Issue

Vol. 59 No. 1 (2019)

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Articles

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