The Result of Some Biomarker of Free Radical-Peroxidation of Lipids and Antioxidant System of Experimental Acute Infarction Model Induced by Coronary Artery Occlusion in Rats

  • Enebish Batbold New Medicine Medical University, Ulanbator, Mongolia
  • Ambaga Miegombo New Medicine Medical University, Ulanbator, Mongolia
  • Sarantsetseg Bandi New Medicine Medical University, Ulanbator, Mongolia
  • Amgalanbaatar Dorjkhuu Mongolian National University of Medical Sciences, Ulan Bator, Mongolia
Keywords: Myocardial infarction, Free radical-pereoxidation of lipids, Antioxidant, Coronary occlusion induced myocardial ischemia, Electron and proton conductance.

Abstract

Background: Recently years, Increasing coronary artery disease, diabetes, and cancer in the worldwide. Epidemiological studies indicate that ischemic heart disease will constitute the major disease-burden worldwide by the year 2020. Myocardial infarction is invariably followed by numerous pathophysiological and biochemical alterations including hyperlipidemia, thrombosis, lipid peroxidation and free radical damage etc., leading to qualitative and quantitative changes of myocardium. Determination of in blood serum and cardiac tissue homogenate levels of cardiac biomarker cardiac specific troponin I and estimation of lipid peroxidation products and some antioxidants enzymes of experimental acute infarction model induced by coronary artery occlusion in rats. Materials and methods: Adult male Wistar rats, weighting approximately 180 to 200±20 gram, used for the experiment. The were divided randomly into 2 groups (6-8 animals in each group). They were distributed as follow: first group (healthy, non treatment), second group (control, experimental acute infarction model induced by coronary artery occlusion in rats,  non treatment). We did coronary occlusion induced myocardial ischemia by Kogan A.K., Ambaga M /1979/‘s method.The experiment is 1st, 3rd, 7th, 14th 21st days blood samples were collected and used to determine in blood serum and cardiac tissue homogenate levels of cardiac biomarker cardiac specific troponin I (CTn-I) and estimation of lipid peroxidation products (MDA, SA, LPO) and some antioxidants enzymes (SOD, GSH, GSH-px) were estimated using standard rat ELISA KIT by enzyme-linked immunesorbent assay. Result: Determination of cardiac troponin-I levels in blood serum of experimental acute infarction model induced by coronary artery occlusion in rats increased by 31.9-58.4% in the 1st, 3rd, 7th, 14th 21st days of the test compared to healthy groups. The cardiac biomarker cardiac specific troponin I is indicative for cardiomyocyte damage and is currently used in the diagnosis and prognosis of myocardial ischemia. Experimental acute infarction model induced by coronary artery occlusion in white rats, it is sufficiently shown that from day 1-21 MDA in plasma increased by 43.9 – 76.3%, plasma SA by 18.3-49.8% and LPO by 12.8-38.3%. This results in necrosis of cells and tissues, dissolving of membrane and stimulation of pathogenesis of disruption of membrane parts. It is also shown that substances such as endogen and antioxidant decreases in heart ischemia. Conclusion: Determination of increased level are lipid peroxidation products (MDA, SA, LPO, CTn-I), and decreased level are antioxidants (SOD, GSH, GSH-px) in blood serum of experimental acute infarction model induced by coronary artery occlusion in rats, it seems to induced of pathogenesis of coronary disease and infarction myocardium while the accumulation of lipid product cause to damage the cell membrane.

References

World Health organization. Global atlas on cardiovascular disease prevention and control 2011. 2011:7-15

WHO, Cardiovascular disease, Available at:http://www.who.int/mediacentre/factssheets/fs317/en/. [Accessed 17 May 2017].

R.Kleemann, S.Zadelaar, T.Kooistra. “Cytokines and atherosclerosis: a comprehensive review of studies in mice” Cardiovasc Res. pp. 360, 2008.

James N.B.M. de Andrade, Junnan Tang, Michael Taylor Hensley, Adam Vandergriff, Jhon Cores, Eric Henry, Tyler A. Allen, Thomas George Caranasos, Zegen Wang, Tianxia Zhang, Jinying Zhang, Ke Cheng. “Rapid And Efficient Production Of Coronary Artery Ligation And Myocardial Infarction In Mice Using Surgical Clips”. Doi:10.1371/Journal.Pone.014322, pp.1-2/11, November 24, 2015.

Hua Li, Yan-hua Xie, Qian Yang, Si-Wang, Bang-Le Zhang. “Protective effect of Paeonol and Danshu Combination on isoproterenol-induced myocardial ischemia in rats”. Plos one. volume number 7, issue 11, pp.1, 2012.

M.Ambaga, A.Tumen-Ulzii. “The full 9 stepped cycle of proton conductance and the time dependent disturbance of clockwise normal flow of electrons, protons during shortage of donators and acceptors”. International Journal of Current Research. Vol. 9, Issue, 08, pp.55902-55903. August.2017.

I.A.Katrukha. “Human Cardiac Troponin Complex. Structure and Functions”. Russian in Uspekhi Biologicheskoi Khimii. vol.53, pp. 1447. 2013.

P.Anversa, EH.Sonnenblick. “Ischemic cardiomyopathy: pathophysiological mechanisms”. Prog Cardiovasc Dis 33:49–70, 1990

AC.Hegstad, K.Ytrehus, R.Myklebust, L.Jorgensen. “Ultrastructural changes in myocardial myocytic mitochondria: crucial step in the development of oxygen radical induced damage in isolated rat hearts”. Basic Res Cardiol, pp.128–138, 1994.

J.Wu, JG.Hecker, N.Chiamvimonvat. “Antioxidant enzyme gene transfer for ischemic diseases”. Adv Drug Deliver Rev pp.61(4):351–363, 2009.

B.Sarantsetseg. “Antioxidant-lipid peroxidation free radical” system, their health science, pathology, significance of pharmacologic treatment”. Research dissertation for Doctor of Medical Science degree. Ulanbator, 1998.

M.Ambaga, A.K. Kogan, A.N. Kudrin “Pharmacotherapeutic action of cardioselective blockers of H1-adrenoreceptors (OF4647 and atenolol), Motley Astragalus, their combinations and Antioxidant-Dibunol in myocardial infarction caused by persistent and transient coronary occlusive ischemia (experimental study)”. Research dissertation for Doctor, Russia, 1984.

D.Ahn, L.Cheng, C.Moon, H.Spurgeon, EG.Lakatta, MI.Talan. “Induction of myocardial infarcts of a predictable size and location by branch pattern probability-assisted coronary ligation in C57BL/6 mice”. Am J Physiol Heart Circ Physiol. pp.286:1201–1207, 2004.

RD.Patten, MJ.Aronovitz, L.Deras-Mejia, NG.Pandian, GG.Hanak, JJ.Smith, et al. “Ventricular remodeling in a mouse model of myocardial infarction”. Am J Physiol. pp.274: 1812–1820, 1998.

LH.Michael, ML.Entman, CJ.Hartley, KA.Youker, J.Zhu, SR.Hall, et al. “Myocardial ischemia and reperfusion: a murine model”. Am J Physiol. pp.269: 2147–2154, 1995.

JA.Tallaj, V.Franco, BK.Rayburn, et al. “Response of doxorubicin-induced cardiomyopathy to the current management strategy of heart failure”. J Heart Lung Transplant. pp.24: 2196-2201, 2005.

B.Sarantsetseg, M.Ambaga. “Lipid peroxidation-antioxidant system”. Ulanbator, pp.23, 1999.

Published
2019-07-25
Section
Articles