Comparison Activities of Peel and Extract of Lime (Citrus amblycarpa) as Antioxidant and Antielastase

  • Stevenie Stevenie Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • Ermy Girsang Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • Ali Napiah Nasution Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
  • I Nyoman Ehrich Lister Faculty of Medicine, Universitas Prima Indonesia, Medan, 20118, Sumatera Utara, Indonesia
Keywords: Lime extract, Antioxidant, Antielastase.

Abstract

Aging of the skin is a physiological process that occurs in all living things, along with increasing age, hormones, nutrition, and exposure to sunlight both directly and indirectly. This process will form Reactive Oxygen Species ROS, free radicals that interfere with one of the most important enzymes in the cell matrix, namely the elastase enzyme. Free radicals can be neutralized by the presence of antioxidants. Lime plants have natural phytochemical compounds that act as antioxidants and anti-aging. This study was to compare the antioxidant activity and anti-elastase from the peel and lime seed extract (Citrus amblycarpa) by conducting H2O2 trapping tests and inhibition of elastase. The test results of antioxidant activity and anti-aging trapping of H2O2 lime seed extract had an IC50 value lower than lime peel extract, with yields of 194.82 μg / ml better than those of lime skin extract of 246.84 μg / ml. The results of the elastase inhibition activity test showed that lime peel extract had a lower IC50 value of 36.94 μg / ml compared to lime seed extract with an IC50 value of 62.39 μg / ml. A lime seed extract has antioxidant activity through better H2O2 trapping compared to lime peel extract while lime skin extract has antiaging activity through inhibition of elastase which is better than lime seed extract.

References

Ganceviciene, Ruta, et al. "Skin anti-aging strategies." Dermato-endocrinology 4.3 (2012): 308-319.

Masaki, Hitoshi. "Role of antioxidants in the skin: anti-aging effects." Journal of dermatological science 58.2 (2010): 85-90.

Durai, Priya Cinna, et al. "Aging in elderly: chronological versus photoaging." Indian journal of dermatology 57.5 (2012): 343.

Fisher, Gary J., et al. "Collagen fragmentation promotes oxidative stress and elevates matrix metalloproteinase-1 in fibroblasts in aged human skin." The American journal of pathology 174.1 (2009): 101-114.

Poljšak, Borut, and Raja Dahmane. "Free radicals and extrinsic skin aging." Dermatology research and practice 2012 (2012).

Harraan, Denham. "Aging: a theory based on free radical and radiation chemistry." (1955).

Mukhopadhyay, Debanjan, et al. "A Sensitive In vitro Spectrophotometric Hydrogen Peroxide Scavenging Assay using 1, 10-Phenanthroline." Free Radicals & Antioxidants 6.1 (2016).

Thring, Tamsyn SA, Pauline Hili, and Declan P. Naughton. "Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants." BMC complementary and alternative medicine 9.1 (2009): 27.

Wittenauer, Judith, et al. "Inhibitory effects of polyphenols from grape pomace extract on collagenase and elastase activity." Fitoterapia 101 (2015): 179-187.

Yaar, Mina, and Barbara A. Gilchrest. "Photoageing: mechanism, prevention and therapy." British Journal of Dermatology 157.5 (2007): 874-887.

Lima, L., and A. Macieira-Coelho. "Parameters of aging in chicken embryo fibroblasts cultivated in vitro." Experimental cell research 70.2 (1972): 279-284.

Liang, Jack N., and Martin T. Rossi. "In vitro non-enzymatic glycation and formation of browning products in the bovine lens α-crystallin." Experimental eye research 50.4 (1990): 367-371.

Widowati, Wahyu, et al. "High Performance Liquid Chromatography (HPLC) analysis, antioxidant, antiaggregation of mangosteen peel extract (Garcinia mangostana L.)." Int J Biosci Biochem Bioinfo 4.6 (2014): 458-66.

Published
2019-07-13
Section
Articles