A Comparative Study of Peel and Seed Extract of Passion Fruit (Passiflora edulis) as Anti Collagenase

  • Surya Hartanto Master Program of Biomedical Sciences, Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia
  • I Nyoman Ehrich Lister Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia
  • Edy Fachrial Laboratory of Molecular Biology, Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia
Keywords: Passiflora edulis, antioxidant, anti collagenase, DPPH.


Natural plant compound is known to have various effects that are beneficial to human health including as antioxidant and anti-aging. The aim of this study was to investigate the antioxidant activity of passion fruit peel and seeds an the activity in inhibiting collagenase. The ethanolic extract of seed and peel of P.edulis was determined by antioxidant activity using DPPH scavenging method and continued with the investigation of anticollagenase activity. The result showed that the IC50 of peel extract and seed extract of P.edulis were 56.11 ± 2.26 µg/mL and 88.25 ± 14.31 µg/mL respectively. The IC50 of anti-collagenase activity of peel and seed extract of P.edulis were 82.53 ± 1.42 µg/mL and 225.40 ± 9.12 µg/mL respectively. From the result, it concluded that peel and seed extract of P.edulis possesses remarkable potency as anti-collagenase activity and have the potential to be developed as anti-aging skin nutraceutical.


I. Binic, V. Lazarevic, M. Ljubenovic, J. Mojsa, and D. Sokolovic, “Skin Ageing : Natural Weapons and Strategies,” Evidence-Based Complement. Altern. Med., vol. 2013, pp. 1–10, 2013.

S. Singh and S. Mishra, “Aging and Nutrition : A Review Article,” IOSR J. Nurs. Heal. Sci., vol. 1, no. 4, pp. 43–47, 2013.

K. Lifie, R. Mansauda, E. Anwar, T. Nurhayati, and E. Anwar, “Antioxidant and Anti-Collagenase Activity of Sargassum plagyophyllum Extract as an Anti-Wrinkle Cosmetic Ingredient,” Pharmacogn J, vol. 10, no. 5, pp. 932–936, 2018.

S. Sharafzadeh, “Medicinal Plants as Anti-Ageing Materials : A Review,” Glob. J. Med. Plant Res., vol. 1, no. 2, pp. 234–236, 2013.

W. D. Ratnasooriya, W. P. K. M. Abeysekera, G. A. S. Premakumara, C. D. T. Ratnasooriya, and S. G. Ratnasooriya, “In vitro anti-collagenase collagenase activity of Sri Lankan low grown orthodox Orange Pekoe grade black tea ( Camellia sinensis L .),” Res. J. Chem. Sci., vol. 7, no. 7, pp. 11–15, 2017.

T. C. Colomeu, D. De Figueiredo, R. D. L. Zollner, L. Maria, and M. Meletti, “Comparison of Antioxidant and Ant proliferative Effect among Four Passiflora Spp .,” J. Agric. Life Sci., vol. 4, no. 2, pp. 1–8, 2017.

C. Irawan, L. Sulistiawaty, and H. Rochaeni, “Evaluation of DPPH free radical scavenging activity of Pometia pinnata from Indonesia,” Pharma Innov. J., vol. 6, no. 8, pp. 403–406, 2017.

H. Jalal, M. A. Pal, H. Hamdani, and M. Rovida, “Antioxidant activity of pomegranate peel and seed powder extracts,” J. Pharmacogn. Phytochem., vol. 7, no. 5, pp. 992–997, 2018.

S. M. R. Hasan, M. Hossain, R. Akter, and M. Jamila, “DPPH free radical scavenging activity of some Bangladeshi medicinal plants,” J. Med. Plant Res., vol. 3, no. 11, pp. 875–879, 2009.

S. R. Kanatt, R. Chander, and A. Sharma, “Antioxidant and antimicrobial activity of pomegranate peel extract improves the shelf life of chicken products Original article Antioxidant and antimicrobial activity of pomegranate peel extract improves the shelf life of chicken products,” Int. J. Food Sci. Technol., vol. 45, pp. 216–222, 2010.

RSR. Benites. ASN. Formagio. EJS Argandona. CRF. Volobuff. LNF. Trevizan. MC. Vieira. MS. Silva, “Contents of constituents and antioxidant activity of seed and pulp extracts of Annona coriacea and Annona sylvatica,” Brazilian J. Biol., vol. 74, no. 3, pp. 685–691, 2015.

S. Ramaiya, J. S. Bujang, M. H. Zakaria, M. Arif, and S. Sahrir, “Sugars , ascorbic acid , total phenolic content and total antioxidant activity in passion fruit ( Passiflora ) cultivars Shiamala Devi Ramaiya , a ∗ Japar Sidik Bujang , a Muta Harah Zakaria , b Wong,” J Sci Food Agric, vol. 93, pp. 1198–1205, 2013.

I. Ahmad, R. Sulistiarini, and L. Rijai, “Antioxidant Activity of Some Selected East Borneo Plants,” Int. J. Publich Heal. Sci., vol. 4, no. 1, pp. 58–62, 2015.

S. Pientaweeratch, V. Panapisal, and A. Tansirikongkol, “activities of Phyllanthus emblica , Manilkara zapota and silymarin : an in vitro comparative study for anti-aging applications,” Pharm. Biol., vol. 54, no. 9, pp. 1865–1872, 2016.