Biosynthesis, Characterization, Optimization of Silver Nanoparticles from Marine S. griseoflavus and Their Role in Improving Antibiotic Susceptibility of Multidrug Resistance Pathogenic Fungi

  • Muneera Al-Otaibi Faculty of Science, Biology Department, Taif University, Taif, KSA
  • Bahig El-Deeb Faculty of Science, Botany Department, Sohag University, Sohag, Egypt
  • Nasser Mostafa Faculty of Science, Biology Department, Taif University, Taif, KSA
Keywords: Marine bacteria, Antifungal activity, Optimization of silver nanoparticles, Biochemical and Molecular characterization.


Green synthesis of silver nanoparticles using marine microorganisms has gained profound interest because of their potential to synthesize nanoparticles of various size, shape and morphology. In the present investigate, 25 silver resistant bacterial isolates were isolated from different sites of red sea cost at  Jeddah city, , bacterial strain BM3 was selected on the basis of its ability to produce stable extracellular silver nanoparticles ( AgNPs). Biochemical and Molecular characterization of this isolate indicated that BM3 strain is belonged to Streptomyces  and identified as S. griseoflavus. The biosynthesis of AgNPs was monitored by UV–Visible spectrum that showed surface plasmon resonance (SPR) peak at 420 nm. Further characterization of synthesized AgNPs was carried out using the XRD, TEM and FTIR spectroscopy. TEM and XRD analysis revealed that the AgNPs synthesized by MB was spherical in shape with a size range of 5-45. nm FTIR confirmed the presence of proteins as the stabilizing agent surrounding the nanoparticles.Optimization of silver nanoparticles synthesis has been studied and the results demonstrated that the biosynthesis of AgNPs by bacterial supernatant increased with the  increasing of  the temperature, pH  and Ag+ ions concentration. The synthesized AgNPs and their combination with different commercial antibiotic were tested against C. albicans and C.krusei.All tested antibiotics showed synergistic inhibition against growth of the pathogenic bacteria. The biocide actions of AgNPs studied using SEM. The results showed damage in membrane and some pits that have been created cause inter cellular components leakage and finally cell death .UV-Vis absorption spectra of AgNPs alone, each antibiotic alone, and AgNPs-antibiotic combinations were studied . The results clearly point out that the synergistic effect of AgNPs-antibiotic combination against pathogenic is mediated by complexes that are produced from the interaction between AgNPs and the antibiotics, instead of individual or additive action of AgNPs or antibiotic .In other words, the pathogenic cells are killed more effectively by “AgNPs-antibiotic complexes” .However, the nature of the interaction between the AgNPs and the antibiotics needs further investigation.


[1]. A.Ahmad , P. Mukherjee, S. Senapati , D. Mandal , M.I Khan , R. Kumar , M. Sastry ” Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum”, Colloid Surface B, vol.28,pp.313–318,2003.
[2]. NB.Ashley , S.Kathryn , AS. Tova , L. Jiangrui ,O.O. Sherine , E.S.Maria,” Nanoparticles Functionalized with Ampicillin Destroy Multiple-Antibiotic-Resistant Isolates of Pseudomonas aeruginosa and Enterobacter aerogenes and Methicillin-Resistant S ”, Advanced Materials,vol.30,pp18-25,2012
[3]. M.Agnihotri ,S. Joshi S, R.Kumar ,S. Zinjard , S.K. Kulkarni ,” Biosynthesis of gold nanoparticles by the tropical marine yeast Yarrowia lipolytica NCIM 3589 ” ,Mater Letter, vol.10 , pp63- 1231.2009.
[4]. K.Chaloupka , Y. Malam, A.M Seifalian , ”Nanosilver as a new generation of nanoproduct in biomedical applications”,Trends Biotechnology , vol. 28,pp 580–588, 2010 .
[5]. X.Chen , H.J.Schluesener,”Nanosilver: a nanoproduct in medical application. Toxicol”, Mater Letter.,vol .176,pp 1–12,2008.
[6]. A.M.Fayaz , K. Balaji ,P.T. Kalaichelvan , R.Venkatesan,” Fungal based synthesis of silver nanoparticles - an effect of temperature on the size of particle,”,Colloid Surf B,vol . 74,pp , 123–126, 2009 .
[7]. J.A.Haggstrom , K.J. Klabunde ,G.L. Marchin ”, Biocidal properties of metal oxide nanoparticles and their halogen adducts”,Nanoscale, vol . 2,pp 399–405,2010 .
[8]. M.Singh ,S. Sing, S.Prasad, I.S. Gambhir ,” Nanotechnology in medicine and antibacterial effect of silver nanoparticles”,Digest. J. Nanomater. Biostructures, vol .3,pp 115–122 ,2008.
[9]. A.Shirley , B. Dayanand, G.D.Syed.” Antimicrobial activity of silver nanoparticles synthesized from novel Streptomyces species” ,Digest Journal Nanomaterials and Biostructures,vol.5, pp 447 – 451, 2010 .
[10]. M.Vanaja , S. Rajeshkumar , K. Paulkumar ,G. Gnanajobitha, C. Malarkodi, G. Annadurai, .” Kinetic study on green synthesis of silver nanoparticles using Coleus aromaticus leaf extract.” , Advances in Applied Science Research, vol .4, pp 50-55,2013 .
[11]. L.D.Vidhya , T. Roshmi, T.V Rintu , E.V. Varghese, J.M. Soniya , E. Radhakrishnan E.” Extracellular synthesis of silver nanoparticles by the Bacillus strain CS 11 isolated from industrialized area.” Biotech ,vol. 4, pp. 121–126, 2014.
[12]. N.Vigneshwaran , A.A Kathe , P.V Varadarajan , R.P.Nachane and R.H. Balasubramanya . “ Silver-Protein(Core-Shell) Nanoparticle Production Using Spent Mushroom Substrate”. Langmuir, vol ,23,pp 7113-7117,2007.
[13]. B. Wang , W.Y.Feng , T.C.Wang . “ Acute toxicity of nano- and micro-scale zinc powder in healthy adult mice.”Toxicol. Lett, vol ,161,pp 115–123 , 2006.
[14]. R.L.Wen , B.X. Xiao, S.S. Qing , YZ. Hai , S.You , O. Yang, Y.B .Chen . “ Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. .” Applied Microbiology and Biotechnology, vol 5 , pp 1115–1122, 2006 .
[15]. X.Chen and H.J. Schluesener .“ Nanosilver: a nanoproduct in medical application.”Toxicol. Lett, vol 10 ,pp 1–12,2008 .
[16]. J.G.Holt , N.R. Krieg , P.H.A. Sneath , J.T. Staley, S.T. Williams .“ Bergey’s Manual of Determinative Bacteriology.”Williams and Wilkins Press, Baltimore, vol , 100,PP 544–551,1994 .
[17]. P. Jeevan, K. Ramya , A.E. Rena .“ Extracellular biosynthesis of silver nanoparticles by culture supernatant of Pseudomonas aeruginosa.” Indian Journal of Biotechnology ,vol 11, pp 72-76,2012.
[18]. S. Kundu, S. Lau, H. Liang 2007.S. “hape-controlled catalysis by cetyltri methyl ammonium bromide terminated gold nanospheres, nanorods, and nanoprisms.” Journal of Physical Chemistry A , vol 13 ,pp 5150–5156, 2007 .
[19]. P.Mulvaney 1996. “Surface Plasmon sepectroscopy of nanosized metal particles.”Langmuir, vol 12 pp 788-800.
[20]. A. Nasrollahi, K.H Pourshamsian , P. Mansourkiaee P. “Antifungal activity of silver nanoparticles on some of fungi.” International Journal of Nano Dimension, vol, 3,pp 233-239, 2011.
[21]. P.S. Pimprikar , A.R. JoshiAR, S.S Kumar , S.K. Zinjarde, M. Kulkarni .“ Influence of biomass and gold salt concentration on nanoparticle synthesis by the tropical marine yeast Yarrowia lipolytica NCIM 3589 .”Colloids and Surfaces, B: Biointerfaces, vol ,74 , pp 309–316, 2009 .
[22]. W.S. Rasband. 1997-2012.Image J. US, National Institutes of Health, Bethesda .
[23]. S. Rajeshkumar , S. Rajeshkumar , C, Malarkodi , K. Paulkumar , M. Vanaja , G. Gnanajobitha . “ Annadurai ,G.Intracellulr and extracellular biosynthesis of silver nanoparticle by using marine bacteria vibrio alginolyticus.” Nanoscience and Nanotechnology: An International Journal ,vol 3, pp 21-25 ,2013 .
[24]. S. Sadhasivam, P. Shanmugam, K. Yun. “Biosynthesis of silver nanoparticles by Streptomyces hygroscopicus and antimicrobial activity against medically important pathogenic microorganisms .” Colloids and Surfaces B.Biointerfaces , vol 3 ,PP 358–362,2010 .
[25]. R. Sanghi and P. Verma .”Biomimetic synthesis and characterisation of protein capped silver nanoparticles.”.Bioresource Technology ,vol 1 , pp 501–504,2009 .