Analysis of Milk Collected From Milk Points for Composition, Adulterants and Microbial Quality in District Swat

Authors

  • Humayun Khan University of Swat
  • Israr Aziz University of Swat
  • Misbahullah Misbahullah University of Swat
  • Jamila Haider University of Swat
  • Ihsan Ud Din Research Officer, Veterinary Research & Disease Investigation Center Balogram, Swat 19200 Pakistan
  • Khurshaid Anwar Senior Research Officer, Veterinary Research & Disease Investigation Center Balogram, Swat 19200 Pakistan
  • Insan Ud Din Senior Research Officer VR & DIC Balogram, Swat
  • Habibunabi Habibunabi Research Officers VR & DIC Balogram, Swat
  • Hanifur Rehman Research Officers VR & DIC Balogram, Swat
  • Anwarud Din Ph.D. Scholar Department of Mathematics, Sun Yet-sen University, China

Keywords:

Milk, Microbial Quality, Adulterants, Salmonella, Colony forming unit, Agar.

Abstract

Milk contains nutrients which are building block for growth and cannot be fulfill by any other food.  In Pakistan Approximately 50 %of the milk produced is consumed as fresh or boiled, one sixth as yoghurt or curd and remaining is utilized for manufacturing of indigenous varieties of milk products such as ice cream, butter, khoa, paneerrabri, kheer, barfi and gulabjamin. A total of 150 samples were collected 50 each from tehsil Babozai, kabal and khwazakhela. The composition was determined through lactoscane milk analyzer, microbial quality through MBRT and adulterant analysis through various procedures. High milk Fat was recorded in tehsil Kabal 5.21% followed by 4.48% in tehsil Khwazakhela and 4.08%in tehsil Babozai. High milk SNF% was 6.92% recorded in tehsil Kabal followed by 6.72% in tehsil Khwazakhela and 6.11% in tehsil Babozai. High milk protein was recorded in tehsil Kabal 3.15% followed by 3.1% in tehsil Khwazakhela and 2.79% in tehsil Babozai.

High quantity of lactose in milk was recorded as 3.04% in tehsil Kabal followed by 2.96% in tehsail Khwazakhela and 2.69% in tehsil Babozai. High amount of added water was observed as 36.22% in tehsil Babozai followed by 34.07% in tehsail Khwazakhela and 31.69% in tehsil Kabal. Boric acid and starch were not detected in all the samples processed. Formalin was detected in all tehsils as 26%, 46% and 36% in tehsil Babozai, kabal and khwazakhela, respectively. On MBRT no sample (0%) was found to be of excellent quality. The good quality was found to be one sample in tehsil khwazakhela (0.6%), 41.33% were of fair quality, 32% were of poor quality and 26% samples were of very poor quality. In tehsil Babozai 20 samples were of very poor quality which was high value among all the three tehsils. The maximum no for fair quality was observed in tehsil Kabal followed by tehsil Khwazakhela. The colony forming unit per milliliter (cfu/ml) for salmonella on salmonella shagilla agar was found 538 in tehsil Babozai followed by 510 in tehsil Kabal and 370 cfu/ml was observed in tehsil Khwazakhela. The data regarding e.coli count (cfu/ml) shows that high number (595)  of colonies of e.coli were observed in milk collected from tehsil Kabal followed by 576  in tehsil Babozai and 480 in tehsil Khwazakhela. Colony forming unit on nutrient agar for various bacterial species showed that maximum number of microorganism were observed in milk samples for tehsil Babozai (590) followed by tehsil Khwazakhela 530 and 475 in tehsail Kabal.

References

[1] Adams, M.R., and Moss, M.O., Food Microbiology. 3rdEdition, The Royal Society of Chemistry. Adulteration of raw milk in the rural areas of Barisal district of Bangladesh. Bang. J. Anim. 41 (2), 112-115
[2] Ali, A., Mahmood, M. S., Hussain, L., Akhtar, M. (2011). "Adulteration and Microbiological Quality of Milk (A Review). Pak J Nutr 12: 1195-1202.
[3] Anushmala, M. K., and B. Niranjan, B. (2012). A comparative microbiological quality assessment of rural and urban milk samples . African Journal of Food Science. 6(21),519-523.
[4] AOAC. (1990). Official methods of analysis of the AOAC, 15th ed. Methods 932.06, 925.09, 985.29, 923.03. Association of official analytical chemists. Arlington, VA, USA.
[5] Barlowska, J., Szwajkowska, M., Litwi’nczuk, Z., and Kr’ol, J. (2011).Nutritional Value and Technological Suitability of Milk from Various Animal Species Used for Dairy Production. Comprehensive reviews in food science and food safety, 10, 291-302
[6] Bryan, F.L., (1988). Risks associated with vehicles of food borne pathogens and toxins. J. Food Prot., 51: 498-508.
[7] Chanda, T., Debnanth, G.K., Hossain, M.E., Islam, M.A., Begum, M.K. (2012). Adulteration of raw milk in the rural areas of Barisal district of Bangladesh. Bang. J. Anim. 41 (2), 112-115
[8] Chatterjee, S.N., Bhattacharjee, I., Chatterjee, S. K., and Chandra, G., (2006).Microbial examination of milk in Tarakeswar, India with special reference to Coliforms. African Jouranal of Biotechnology,5(15),1383-1385.
[9] Deman, J.C., Rogosa, M., and Sharp, M.E., (1960). Amedium for the cultivation of lactobacilli., J. Appl.Bacteriol., 23: 130-135.
[10] Faraz, Lateef, A. M,. Mustafa M. I., Akhtar, P,. Yaqoob, M., and Rehman, S. (2013). Detection of adulteration, Chemical composition and hygienic status of milk supplied to various canteens of Educational Institutes and Public places in Faisalabad. J Anim Plant Sci, 23( 1),119-124
[11] Hamid, I., Sikandar, K. S., Muhammad, A., Abdul, Q. K., Asim, K., Saifullah, Muhammad, N. A. Q. J., Shahzad, M.(2013).Microbial analysis and quality control of milk collected from various districts of Khyber Pakhtunkhwa. Research Article ,CODEN, IJPRNK Shahzad Munir, IJPRBS, 2(4): 243-252
[12] Javaid, S. B., Gadahi, J. A., Khaskeli, M., Bhutto, M. B., Kumbher, S., and Panhwar, A. H. (2009).Physical and chemical quality of market milk sold at Tandojam, Pkaistan. Pakistan Vet. J. 29(1)27-31.
[13] Keter, G., 1975. The bacterial flora in aseptically draw milk neth. Milk dairy J., 28: 220-237
[14] Lateef, M., A. Faraz, M.I. Mustafa, P. Akthar and M.K. Bashir, 2009. Detection of adulterants and chemical composition of milk supplied to canteens of various hospitals in Faisalabad city. Pak. J. Zool., 9: 139- 142.
[15] Lateef, M., Faraz, A., Mustafa, M. I., and Bashir, M. K., (2009). Detection of Adulterants and Chemical Composition of Milk Supplied to Canteens of Various Hospitals in Faisalabad City. Pakistan J.Zool., 9:139-142.
[16] Loudon, and Irvine, (1986). Deaths in childhoods from the eighteenth century to 1935. Med. History, 30: 1- 41.
[17] Mabrook, M.F., and Petty,M.C., (2003). Effect of composition on the electrical conductance of milk.J.Food Eng., 60:321-325.
[18] Marcus, I. A., (1979). Disease prevention in America: From a local to a national out look, 1880-1910. Bull. History Med., 53: 184-203.
[19] Maxcy, R.B., (1967). Nature and growth response of themicroflora of pasteurized, packaged milk, J. MilkFood Tech., 30: 213-220.
[20] Muir, D.D., (1996). The shelf-life of dairy products: Rawmilk and fresh products. J. Society of Dairy Tech.,49: 44-50.
[21] Okeke, S. K., Abullahi, O. I., and Makun, H. A., and Okeke, U. K. (2016). Physico-Chemical and Nutritional Qualities of Dairy Cattle Products. G.J.L.S.B.R.,2(2),8-13.
[22] Rideout, T. C., Liu Q, Wood, p., and Fan, M. Z., (2008). Nutrient utilization and intestinal fermentation are differentially affected by consumption of resistant starch varieties and conventional fibres in pigs.Br.J.Nut., 99:984-992.
[23] See, A.S., A.B. Salleh, F.A. Bakar, N.A. Yusof, A.S. Abdulamir and L.Y. Heng, (2010). Risk and health effect of boric acid. Am. J. Applied Sci., 7: 620-627.
[24] Shrishti, N., P. Rakesh, P., and Nishant, R. (2013) Analysis Of Milk Quality, Adulteration And Mastitis In Milk Samples Collected From Different Regions Of Dehradun. International Journal of PharmTech Research CODEN (USA) .5 (2), 359-364.

Downloads

Published

2017-09-25

How to Cite

Khan, H., Aziz, I., Misbahullah, M., Haider, J., Ud Din, I., Anwar, K., Ud Din, I., Habibunabi, H., Rehman, H., & Din, A. (2017). Analysis of Milk Collected From Milk Points for Composition, Adulterants and Microbial Quality in District Swat. American Scientific Research Journal for Engineering, Technology, and Sciences, 36(1), 95–108. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/3397

Issue

Section

Articles