Characterization of Enterobacter cloacae Strains Isolated from different areas of Basrah Hospital by Multilocus Sequence Typing Method(MLST)
Keywords:
Enterobacter cloacae, Phylogenetic tree, MLST.Abstract
In this study we used seven genes of multilocus sequence typing(MLST) to detect genetic diversity among nine isolates of Enterobacter. The results showed that the isolates identified into the species Enterobacter cloacae belong to different strains after sequenced and analyzed of seven MLST genes. the phylogenetic trees were drawn for each gene and for all genes based on neighbor-joining method after sequences alignment in Clustal W,whereas the trees divided into groups.
References
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[2] Diancourt,L. , Passet ,V. ,Verhoef ,J., Grimont,P.A. and Brisse,S. Multilocus sequence typing of K.pneumoniae nosocomial isolates . Journal of Clinical Microbiology, 43:4178-4182.2005.
[3] Vimont, S., Mnif,B., Fevre,C. and Brisse,S. Comparison of PFGE and multilocus sequence typing for analysis of K. pneumoniae isolates . Journal of Medical Microbiology , 57:1308-1310.2008.
[4] Maiden ,M.C., Bygraves ,J.A., Feil ,E., Morelli ,G., Russell ,J.E., Urwin,R. et al. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proceedings of the National Academy of Sciences of the United States of America, 95:3140-3145.1998.
[5] Noller,A.C., McEllistrem,M.C., Stine,O.C., Morris,J.G., Boxrud,D.J., Dixon,B. et al. Multilocus sequence typing reveals a lack of diversity among Escherichia coli O157:H7 isolates that are distinct by pulsed –field gel electrophoresis. Journal of Clinical Microbiology, 41:.675-679.2003.
[6] Tartof, S.Y., Solberg,O.D., Manges,A.R. and Riley,L.W. Analysis of a uropathogenic Escherichia coli clonal group by multilocus sequence typing . Journal of Clinical Microbiology, 43:5860-5864.2005.
[7] Urwin,R. and Maiden,M.C. Multilocus sequence typing :a tool for global epidemiology. Trends in Microbiology, 10:479-487.2003.
[8] Enright , M.C., Day ,N.P.J., Davies ,C.E., Peacock,S.J. and Spratt,B.G. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus . Journal of Clinical Microbiology, 38:1008-1015.2000.
[9] Enright, M.C.and Spratt,B.G. Multilocus sequence typing. Trends in Microbiology, 7:482-487.1999.
[10] Siripattanapipong , S . ; Mungthin ,M., Tan-Ariya,P. and Leelayoova,S . Multilocus sequence typing(MLST) and its applications in epidemiological studies for parasitic infections. Parasitology ResearchTrends,pp.99-117.2010.
[11] Maiden,M.C.J. Multilocus sequence typing of bacteria. Annual Review of Microbiology , 60:561-588.2006.
[12] Enright,m.C. and Spratt,B.G. A multilocus sequence typing scheme for Streptococcus pneumoniae:identification of clones associated with serious invasive disease. Journal Microbiology, 144:3049-3060.1998.
[13] Forsythe ,S.J., Dickins,B. and Jolley,K.A. Cronobacter,the emergent bacterial pathogen Enterobacter sakazakii comes of age;MLST and whole genome sequence analysis. Journal.BMC Genomics, 15:1-14.2014.
[14] Joseph ,S., Sonbol ,H., Hariri ,S., Desai ,P., McClelland,M. and Forsythe,S.J . Diversity of the Cronobacter genus as revealed by multilocus sequence typing. Journal of Clinical Microbiology, 50:3031-3039.2012.
[15] Joseph ,S., Cetinkaya ,E., Drahovska ,H., Levican,A., Fiqueras,M.J. and Forsythe,S.J. Cronobacter condiment sp. nov.,isolated from spiced meat,and Cronobacter universalis sp. nov., a species designation for Cronobacter sp.genomospecies 1,recovered from aleg infection,water and food ingredients. International Journal of System Evolutionary Microbiology, 62:1277-1283.2012.
[16] Helgason ,E., Tourasse ,N.J., Meisal ,R., Caugant,D.A. and Kolsto,A.-B. Multilocus sequence typing scheme for bacteria of bacillus cereus group. Journal of Applied and Environmental Microbiology, 70:191-201.2004.
[17] Miyoshi-Akiyama ,T., Hayakawa ,K., Ohmagari ,N., Shimojima,M. and Kirikae,T . Multilocus sequence typing (MLST) for characterization of Enterobacter cloacae. PLoS ONE, 8:1-10.2013.
[18] Girlich ,D., Poirel,L. and Nordmann,P . Clonal distribution of multidrug-resistant Enterobacter cloacae . Journal Diagnostic Microbiology and Infectious Disease, 81:264-268.2015.
[19] Abbas,Y.A. and.Radhi,G.F. Identification of Enterobacter spp.by 16SrRNA gene sequencing in Basrah province/Iraq .International Research Journal of Natural Sciences, 4:26-39.2016.
[20] Rozas ,J., Sanchez-DelBarrio ,J.C., Messeguer,X. and Rozas,R. DnaSP,DNA polymorphism analyses by the coalescent and other methods. Bioinformatics, 19:2496-2497.2003.
[21] Marcelletti, S., Ferrante,P. and Scortichini,M. Multilocus sequence typing reveals relevant genetic variation and different evolutionary dynamics among strains of Xanthomonas arboricola pv.juglandis. Journal Diversity, 2:1205-1222.2010.
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Published
2017-04-18
How to Cite
Al-Kanaany, G., & Al-Khafaji, Y. (2017). Characterization of Enterobacter cloacae Strains Isolated from different areas of Basrah Hospital by Multilocus Sequence Typing Method(MLST). American Scientific Research Journal for Engineering, Technology, and Sciences, 30(1), 213–225. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/2859
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