Genetic Characterization of Indigenous Sudanese Cattle Using FSHR and LHR Genes
Keywords:
Sudanese cattle, RFLP, FSHR, LHR.Abstract
The aim of this study was to estimate the genotypic and allelic frequencies of the two polymorphisms located in the receptor of Follicle Stimulating Hormone (FSHR) and Luteinizing Hormone (LHR). One hundred and sixteen blood samples were collected from three native Sudanese dairy ecotypes. The studied samples include 32, 34 and 50 cows from Butana, Kenana and Erashy cattle types, respectively. The DNA was extracted following standard methods. The purified DNA was subjected to PCR-RFLP techniques to identify polymorphisms of the FSHR and LHR genes in the three Sudanese cattle ecotypes. The amplified fragments of FSHR (306 bp) were digested with restriction enzymes ALu resulting in 243 and 63 bp fragments. At exon10in the FSHR gene, all genotyped cows in the investigated native Sudanese population were homozygous for AA genotype. The restriction endonuclease Hhal allowed the identification of three genotypes of the LHR gene at exon 11 among the different cattle ecotypes: The TT, CT and the CC genotypes. The observed genotypic frequencies for LHR gene in Kenana were 33.3% for TT, 41.7% for CT and 25% for CC genotypes. In Butana cows, the frequencies were 18.7 for TT, 50% for CT and 31.3% for CC. In Erashy cattle the frequencies were 33.3% for TT, 50% for CT and 16.7% for CC. It was concluded that the allele of FSHR gene among the tested animals of Kenana, Butana and Erashy is monomorphic while the LHR allele is polymorphic.
References
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[27] B.C. Hernandez-Cruz, P. Cervantes-Acosta, F. Montiel-Palacios, R Canseco-Sedano , and A. Carrasco-Garcia,. ’’Allelic variants of FSHR gene in cows of different genotypes Mixco’’ Animal and Veterinary Advances, 8: 2489-2494, 2009.
[28] A.Latronico, and I. Arnhold. ‘’Inactivating mutations of LH and FSH receptors from genotype to phenotype.’’ Pediat. Endocrinol. Rev, 4: 28-31. PM: 17021580, 2006.
[29] ME. Carvalho LGG Figueiredo. EP. Marson P . Ripamonte, Caracterização da heterozigose no gene do receptor do hormônioluteinizante (LHR) emanimais da raça Nellore. In: Simpósio da SociedadeBrasileira de Melhoramento Animal. Anais do V SimpósioNacional da SociedadeBrasileira de Melhoramento Animal (SBMA), Pirassununga 2004.
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[2] M.P. Milazzotto, “Mutações no gene do receptor do hormônioluteinizante (LHR) bovino e associação com precocidade sexual emfêmeasBosprimigeniusindicus (Nelore).’’M.Sc. thesis, Instituto de Biociências, UniversidadeEstadualPaulista, Campus de Botucatu, Botucatu, SP, Brazil, 2001.
[3] F. Campagnari , ‘’Novasvariantesmoleculares dos genes dos receptores do hormonioliberador de gonadotrofinas (GnRHR) e do hormoniofoliculoestimulante (FSHR) emfemeasBosprimigeniusindicus (Nelore).’’ M.Sc. Thesis, Instituto de Biociencias, UniversidadeEstadual, Paulista, Campus de Botucatu, Botucatu, SP, Brazil, 2001.
[4] E.P. Marson, J.B. Ferraz, F.V. Meirelle, J.C. Balieir, J.P. Eler, L.G. Figueiredo, and G.B. Mouro, ‘’Genetic characterization of European-Zebu composite bovine using RFLP markers.’’ Genet. Mol. Res, 2005, 4: 496-505.
[5] D.G. Bradley, D.E. MacHugh, P. Cunningham, and R.T. Loftus, ‘’Mitochondrial diversity and the origins of African and European cattle.’’Proc. Nat. Acad. Sci. USA.1996, 93: 5131-5135.
[6] , D.E. Mac-Hugh, R.T. Loftus, D.G. Bradley, P.M. Sharp, andE. P. Cunningham, ‘’Microsatellite DNA variation within and among European cattle breeds.’’Proc Royal Soc1994, 256:25-31.
[7] R.T. Loftus , D.E. MacHugh, D.G. Bradley, Sharp, P.M. and, E.P. Cunningham ,‘’Evidence for two independent domestications of cattle.’’ Proceedingof the National Academy of Science USA, 1994, 91, 119–144.
[8] O.Hanotte, C.L. Tawah, , D.G. Bradley, M. Okomo, Y. Verjee, , J. Ochieng , and, J.E.O. Rege, ‘’Geographic distribution and frequency of a taurineBostaurus and an indicineBosindicus Y specific allele amongst sub-Saharan African cattle breeds.’’. Molecular Ecology, ,pp 387 – 396, 2002.
[9] O. Hanotte, Y. Ronin, M. Agaba, P. Nilsson, A. Gelhaus, , R . Horstmann , Y. Sugimoto, S. Kem , J. Gibson, A. Korol, M. Sollerand. A. Teale, Mapping of quantitative trait loci controlling trypanotolerance in a cross of tolerant West African N'dama and susceptible East African Boran cattle.’’ Proc. Natl. Acad. Sci.USA , 2003, 100 (13): pp 7443 – 7448.
[10] A. M. Dierich, L.Ram Sairam, G.M. Monaco, A. Fimia, M. Gansmuller, LeMeur, and P. Sassone-Corsi, ‘’Impairing follicle-stimulating hormone [FSH] signalling in vivo; Targeted disruption of the FSH receptor leads to aberrant gametogenesis and hormonal imbalance.’’ Proc. Nat.Acad. Sci., USA, 1998, 95:136113617.PMI:9811848
[11] M. J. Simoni, W. Gromoll, A. Hoppner, T. Kamischke, D. Krafft, E. Sthle.and Nieschlag, ‘’Mutational analysis of the follicle-stimulating hormone (FSH) receptor in normal and infertile men: Identification and characterization of two discrete FSH receptor isoforms.’’ J. Clin. Endocrinol.Metab, 84: 75-755, 1999
[12] A.Houde, A. Lambert, J. Saumande, D.W. Silversides, and J.G. Lussier, ‘’Structure of the bovine follicle-stimulating hormone receptor complementary DNA and expression in bovine tissues.’’ Molecular Reproduction and Development, 39: 127-135, 1994.
[13] M.F. Allan, R.M. Thallman, R.A.; Cushman, S.E. Echternkamp and S.N. White et al. ‘’Association of a single nucleotide polymorphism in SPP1 with growth traits and twinning in a cattle population selected for twinning rate.’’ J. Anim. Sci, , 85: 341-347, 2007.
[14] L.P.M.K. Vasconcellos, D.D. Tambasco, A.Talhari, P. Pereira, L.L. Coutinho, and L.C.A. Regitano, ‘’Genetic charaterization of Aberdeen Angus cattle using molecular markers.’’ Genet. Mol. Biol, 26:133-137, 2003.
[15] S.E.M. Almeida, M.S.N. Machado, C.S. Steigleder, C.L. Gama, M.H. Hutz, L.E. Henkes, J.C.F. Moraes, and T.A. Weimer, ‘’Genetic diversity in Brazilian bovine herd based on four microsatellite loci.’’ Genet. Mol. Biol, 23: 345-350, 2002.
[16] E.P. Marson, J.B. Ferraz, F.V. Meirelles, J.C. Balieiro and J.P. Eler, ‘’Effects of polymorphisms of LHR and FSHR genes on sexual precocity in a Bostaurus x Bosindicus beef composite population.’’ Genet. Mol. Res, 7(1): 243-251, 2008.
[17] P.Rahal, A.C. Latronico, M.B.F.Kohek, R.F.S. de Lucia, M.P. Milazzotto, M.B. Wheeler, J.B.S. Ferraz, J.P. Eler, J.F. Garcia, ‘’Polymorphisms in the bovine follicle-stimulating hormone receptor gene.’’ Anim. Genet, 31: 280-281, 2000.
[18] J.Gromoll, M. Simoni, V. Nordhoff, H. Behre, C. de. Geyter, And E. Nieschlag, ‘’Functional and clinical consequences of mutations in the FSH receptor.’’ Mol. Cell. Endocrinol, 125: 177-182, 1996.
[19] I. Huhtaniem, and K. Aittomaki, ‘’Mutations of follicle-stimulating hormone and its receptor: Effects on gonadal function.’’ Eur. J. Endocrinol, 138: 473-481, 1998.
[20] P.Hyttel , T.Greve, T .Fair , SCJ.Hulshof, and,MP. Boland ‘’Oocyte ultrastructure in bovine primordial to early teriary follicles’’ . Brain Structure and Function (Print Edition), 195, 327-336. 1997.
[21] M.A. Beg, D.R. Bergfelt, K. Kot, M.C.Wiltban, and O.J. Ginther, ‘’Follicular-fluid factors and granulosa-cell gene expression associated with follicle deviation in cattle.’’ Biology of Reproduction, 64: 432-441, 2001.
[22] O.J. Ginther, D.R. Bergfelt, M.A. Beg, and K. Kot, ‘’Follicle selection in cattle: role of luteinizing hormone.’’ Biol Reprod, 64: 197-205, 2001.
[23] R. Sartori, P.M. Fricke, J.C.P. Ferreira, O.J .Ginther, and M.C. Wiltbank, ‘’Follicular deviation and acquisition of ovulatory capacity in bovine follicles.’’ Biol. Reprod, 65:1403-1409, 2001.
[24] C.M. Barros, R. Ereno, R.A.L. Simões, P. Fernandes , J. BuratiniJr, and M.F.G. Nogueira, ‘’Use of knowledge regarding LH receptors to improve superstimulatory treatments in cattle.’’ Reprod Fertil Dev, 22:132-137 , 2010.
[25] DK. Lahiri and JI. Nurnberger,‘’A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies.’’ Nuc Acid Res, 19;5444 , 1991.
[26] D. S. Falconer, and T. F. C. Mackay, Introduction to Quantitative Genetics, Ed. 4.Longman, Harlow, Essex, United Kingdom.1996.
[27] B.C. Hernandez-Cruz, P. Cervantes-Acosta, F. Montiel-Palacios, R Canseco-Sedano , and A. Carrasco-Garcia,. ’’Allelic variants of FSHR gene in cows of different genotypes Mixco’’ Animal and Veterinary Advances, 8: 2489-2494, 2009.
[28] A.Latronico, and I. Arnhold. ‘’Inactivating mutations of LH and FSH receptors from genotype to phenotype.’’ Pediat. Endocrinol. Rev, 4: 28-31. PM: 17021580, 2006.
[29] ME. Carvalho LGG Figueiredo. EP. Marson P . Ripamonte, Caracterização da heterozigose no gene do receptor do hormônioluteinizante (LHR) emanimais da raça Nellore. In: Simpósio da SociedadeBrasileira de Melhoramento Animal. Anais do V SimpósioNacional da SociedadeBrasileira de Melhoramento Animal (SBMA), Pirassununga 2004.
[30] E. J. Gardner, Simmons, and P. Snustad. Principios de Genetica.4th Edn. Mexico: Limusa,Wiley, 2002, pp.1-776.
[31] A. Wunsch, B. Sonntag and M. Simoni, ‘’Polymorphism of the FSH receptor and ovarian response to FSH.’’ Ann. Endocrinol ,68: 160-166, 2007.
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2016-08-19
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N. Omer, N., Gornas, N., A. Rahmatalla, S., & A. Ahmed, M.-K. (2016). Genetic Characterization of Indigenous Sudanese Cattle Using FSHR and LHR Genes. American Scientific Research Journal for Engineering, Technology, and Sciences, 24(1), 1–9. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/1185
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