The Influence of Estradiol and HDL-Cholesterol levels on Breast Cancer in Pre-menopause and Menopause Women and its Correlation with BRCA1 Gene C61G Mutation

Sahna Ferdinand Ginting, Sumaryati Syukur, Sanusi Ibrahim, Djong Hon Tjong


Estradiol hormones will increase the risk for breast cancer. About 5% to 10% of breast cancer patients bring with them germ line mutation. The aim of this study is to evaluate the estradiol and HDL-cholesterol levels and the correlation with BRCA1 gene C61G mutation. This research included 120 blood samples from normal females and 45  tissue samples.  The results were that subjects with breast cancer in premenopause group indicating higher estradiol levels compared with menopause group. Highest HDL-cholesterol levels were found in 30-39 year group, and the lowest levels were found in the 50-59 years old group. Of all subject, only 32,5% samples with ER+/PR+, while ER-/PR- were found in 37,5%.  HER-2/neu(+3) was found in 55,5% samples. For ER+/PR+ increased in the age group of 40-49 years, while ER-/PR- increased in the age group of 40-49 years  ( pre-menopause > menopause ).  HER2/neu (+3) was higher in pre-menopause  than in menopause group.  DNA sequencing results with no mutation or polymorphism in this SNP. There was significant difference between pre-menopause group and menopause group in estradiol and HDL-cholesterol content, ER+/PR+ was highest in pre-menopause.

The highest  ER-/PR- was found in in pre-menopause group, and the highest Her2/neu (+) was found in pre-menopause. BRCA1 gene C61G mutation was not found, it was suggested that polymorphism is occured in another different location.  


Estradiol; HDL cholesterol; ER/PR; HER2/neu; BRCA1 mutation C61G.

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Azhar,M,Natalia, D.,Syukur,. S, Vovien, Jamsari, (2015) Gene Fragments that encodes inulin hydrolysis enzyme from genomic Bacillus licheniformis:Isolation by PCR technique using new primers International, Journal of Biological Chemistry 9 (2) ,pp.59,

Abbas AK, Lichtman AH, Pillai S. 2007. Immunity tu Tumors, in Cellular and Molecular Immunology, 6th Ed. Philadphia: WB Saunders, pp. 397-418.

Bolton Jl and Thatcher GRJ. 2008. Potential Mechanisms of Estrogen Quinone Carcinogenesis, Chem Res Toxicol, pp. 21 (1):93-101

Baudi F, Quaresima B, Grandinetti C, Cuda G, Faniello C, Tassone P, Barbieri V Bisegna R, Ricevuto E, Conforti S, Viel A, Marchetti P, Ficorella C, Radice P, Costanzo F,Venuta S.2001. Evidence of a Founder Mutation of BRCA1 in a Highly Homogeneous Population from Southern Italy with Breast/Ovarian Cancer. Hum Mutan; 18(2), pp. 163.

Donal P. Mc Downell, Sungkee Park, Matthew T. Goulet, Jeff Jasfer, Suzane E, Wardell, Ching-Yi Chong, Jhon D. Haris, Jhon D Guyton, and Erik R. Nelson. 2014. Obesity, Cholesterol Metabolism and Breast Cancer Pathogenesis Cancer Res, pp. 4976-4982

Ellis IO, Schinitt SJ, Sastre GX, et al. 2003. Invasive Breast Carcinoma in World Health Organization Classification of Tumors Pathology & Genetics Tumors of the Breast & Female Genital Organs. IARC, pp. 13-59

Ginting, S,F, Syukur S, Ibrahim, S, Tjong, D, H, and Fachrial E, 2015, Lipid profile and estradiol analysis between pre-menopose and post-menopause woman in Medan areas of North Sumatra, Indonesia, journal of Chemical and Pharmaceutical Research,7(10), 505-508

Ibrahim AM. Ali RM. Ali MM, Azary SS, Hassan AK. 2011. Hormonal Changes in Egyptian Patients Suffering from Prostate Cancer and Benign Prostate Hyperplasia. NY Scj; pp. 4(3):18-26

Knudson AG, 2001. Two Genetic Hits (more or less) to Cancer. Nat Rev Cancer 1, pp. 157-162

Lacey J, Kreimer A , Buys S, Marcus P, Chang S , Leitzmann M Hoover R Prorok P, Berg C., Hartge P, Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening Trial Project Team. Breast cancer epidemiology according to recognized breast cancer risk factors in the Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening Trial Cohort.BMC Cancer 2009, pp. 9:84-92

Lee A, Park WC, Yim HW, Lee MA, Park G, Lee KY. 2007 Expression of c-erbB2, cyclin D1, and Estrogen Receptor and their Clinical Implication in the Invasive Ducal Carcinoma of the Breast. Japan Journal of Clinical Oncology 37 (9), pp708-714 Meads,C.Ahmed,I, Riley,R.D.2012. A Systematic Review of Breast Cancer Incidence Risk Prediction Models with Meta Analysis of their Performance. Breast Cancer ResTreat; 132(2), pp. 365-377

Syukur S, Syafrizayanti, Zulaiha S, (2017). Virgin Coconut 0il Increase HDL, lower triglyceride and fatty acids profile in blood Serum of mus musculus, Research journal of Chemical and Pharmaceutical Research 8(2) pp 1077.

Sihombing, B., Jamaludin, Cong, D,H, Ibrahim, S., Syukur, S., (2015) Immunohistohemical detection of p53 protein as a prognostic indicator in prostate carcinoma, Journal of Chemical and Pharmaceutical Research 7(9)

Grzybowska E, Zientek H, Jasinska A. High Frequency of Recurrent Mutations in BRCA1 and BRCA2 Genes in Polish Families with Breast and Ovarian Cancers. Human Mutan 2000, pp. 16: 482–90.


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