The Influence of Estradiol and HDL-Cholesterol levels on Breast Cancer in Pre-menopause and Menopause Women and its Correlation with BRCA1 Gene C61G Mutation

Sahna Ferdinand Ginting, Sumaryati Syukur, Sanusi Ibrahim, Djong Hon Tjong

Abstract


Estradiol hormones will increase the risk for breast cancer. About 5% to 10% of breast cancer patients bring with them germ line mutation. The aim of this study is to evaluate the estradiol and HDL-cholesterol levels and the correlation with BRCA1 gene C61G mutation. This research included 120 blood samples from normal females and 45  tissue samples.  The results were that subjects with breast cancer in premenopause group indicating higher estradiol levels compared with menopause group. Highest HDL-cholesterol levels were found in 30-39 year group, and the lowest levels were found in the 50-59 years old group. Of all subject, only 32,5% samples with ER+/PR+, while ER-/PR- were found in 37,5%.  HER-2/neu(+3) was found in 55,5% samples. For ER+/PR+ increased in the age group of 40-49 years, while ER-/PR- increased in the age group of 40-49 years  ( pre-menopause > menopause ).  HER2/neu (+3) was higher in pre-menopause  than in menopause group.  DNA sequencing results with no mutation or polymorphism in this SNP. There was significant difference between pre-menopause group and menopause group in estradiol and HDL-cholesterol content, ER+/PR+ was highest in pre-menopause.

The highest  ER-/PR- was found in in pre-menopause group, and the highest Her2/neu (+) was found in pre-menopause. BRCA1 gene C61G mutation was not found, it was suggested that polymorphism is occured in another different location.  


Keywords


Estradiol; HDL cholesterol; ER/PR; HER2/neu; BRCA1 mutation C61G.

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