Isolation and Molecular Identification of Bacillus Subtilis in Petroleum-Contaminated Soil
Keywords:
B. subtilis, petroleum-contaminated[1] soil, bioremediationAbstract
Petroleum pollution poses a significant threat to soil health and ecosystem stability, particularly in oil-producing regions. This study aimed to isolate and identify hydrocarbon-degrading bacteria from contaminated soil samples. Four soil samples were collected, preserved, and analyzed for microbial growth. The isolate with the highest colony-forming unit count was subjected to Gram staining, biochemical tests, and molecular identification using 16S rRNA gene sequencing. The isolate with the highest colony-forming unit count was subjected to Gram staining, biochemical tests, and molecular identification using 16S rRNA gene sequencing. The bacterium was confirmed as Bacillus subtilis, showing positive catalase and motility reactions, and negative oxidase activity. BLAST analysis revealed 100% sequence identity with B. subtilis strain 17A. These findings validate the potential of B. subtilis as a native bioremediation agent for petroleum-contaminated soils, offering a sustainable solution for environmental restoration.
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