Investigation of Chemical Composition, Antimicrobial and Antioxidant Activities of Allium Wallichii Kunth (Garlic) Bulb

Dr. Mi Mi Yee


Garlic can high fully be called one of nature’s wonderful plants with leading power. In this research work, the bulb of Allium wallichii Kunth (garlic) was collected from Panglong area, southern Shan state. It is known that garlic (Allium wallichii Kunth.) possesses antimicrobial, antiprotozoal, antimutagenic, antiplatelet and antihyperlipidermic properties. From the presence research work, the following inference could be deduced. The preliminary phytochemical investigation indicated that alkaloids, -amino acids, flavonoids, saponins, phenolic compounds, glycosides were present and tannin, starch and reducing sugar were absent.  The nutritional value of garlic were determined by AACC method, it was show that (60.22%) of moisture, (1.25%) of ash, (5.15%) of proteins, (0.69%) of fibre, (0.1%) of fat and (32.63%) of carbohydrate. Elemental analysis of garlic sample was determined by EDXRF method. It was found that Ca (56.489%), K (40.207%), S (0.927%), Si (0.926%), Mn (0.656%), Fe (0.434%), Cu (0.131%), Zn (0.130%), and Rb (0.100%) were present in garlic. The vitamin C contents was observed (27.455mg) in the samples by using titration method. Antioxidant activities of two solvent extracts, such as ethanol and water extracts, were determined by DPPH assay. Water extract exhibits higher DPPH radicals scavenging activity (IC50 = 1.21gmL-1) than that of ethanol extract (IC50 = 2.10 gmL-1). In vitro, antimicrobial activity of some crude extracts (EtOH, MeOH and H2O) of garlic was screened by agar-well diffusion method. H2O extract of garlic was found to exhibit potent of antimicrobial activity (25mm) against on B.sub, S.sureous, P.seudomonus, B.pumalis, Candida and E.coli.


Allium wallichii Kunth; Phytochemical; DPPH and EDXRF elemental Main text.

Full Text:



.AACC. 2000. Approved methods of the American Association of Cereal Chemists, 10th Ed. AACC, St. Paul, MN, USA.

.Amonkar SV, Banerji A. Isolation and characteri-zation of larvicidal principle of garlic. Science 1971;174:1343.

.Ashokkumar, R. and Ramaswamy, M. (2013). “Determination of DPPH Free Radical Scavenging Oxidation Effects of Methanolic Leaf Extracts of Some Indian Medicinal Plant Species”, Journal of Chemical, Bological and Physical Sciences, 3 (2), 1273 – 1278

.Del-Rio A Obdulio BG,Castillo J.Marin PR,Ortuno A(1977),Uses and properties of citrus flavonoids.J.Agric.Food Chem. 45:4505-4515.

.Gupta R, Sharma NK. A study of the nematicidal activity of allicin: an active principle in garlic, Allium sativum L., against root-knot nematode, Meloidogyne incognita. (Kofoid and White 1919) Chitwood 1949. Int J Pest Manag 1993;39:390-2.

.Kaufman PB, Pennacchini L, McKenzie M, Hoyt JE. Good and bad uses of these compounds by humans. In: Kaufman PB, Cseke LJ, Warber S, Dukes JA, Brielmann HL (eds). Natural products from plants. New York: CRC Press, 1999. p.123-56.

.Lawson LD. Bioactive organosulfur compound of garlic and garlic products: role in reducing blood lipids. In:Kinghorn AD, Balandrin MF, editors. Human medicinal agents from plants. Washington: American Chemical Society,1993. p.306-30. (ACS Symposium Series, n. 534).

.Lanzotti, V., Scala, F., and bonanomi, G. (2014). Compounds from Allium species with cytotoxic and antimicrobial activity. Phytochemistry Reviews, 13, 769-791.

.Liu RH (2004) .Potential synergy of phytochemicals in cancer prevention mechanism of action .J.Nutr.,134:3479S-3485S.

.Michahelles E. Über Neue wirkstoffe aus knoblauch (Allium sativum) und kuchenzwiebel (Allium cepa L.) [dissertation]. München: Ludwig-Maximilians Univiversität München, 1974. p.141.

.Okwu,DE (2004) Phytochemical and vitamin content of indigenous spices of South Eastern Nigeria .J.Sustain.Agric.Environ, 6:30-34.

.Salah W, MillerNj,Pagauga G,Tijbung,BolwellGP,Rice E,EvansC(1995)Polyphenolic flavonoids as acavenger of aqueous phase radicals and chainbreaking antioxidants. Arch.Biochem. Biol.2:339-346.

.Santos, P.R.V, Oliveria, A.C.X, Tomassini, T.C.B. (1995) "Controle Microbiogicode Produtos Fitoterapicos", Rev Farm Bioquim, vol.31, 1985,pp.35-38.

.Thabrew MI, Hughes RD, McFariane IG (1998). Antioxidant activity of Osbeckia aspera. Phtother.Res.12:288-290.

.Ujowundu, C. O., F.N. Kalu, E.C. Nwosunjoku, R.N.Nwaoguikpe, R.I. Okechukwu and K.O. Igwe. 2011 iodine and inorganic mineral contents of some vegetables, spices and grains consumed in Southeastern Nigeria. Afri. J. Biochem. Res. 5(2):57-64.

.Vogel, A.I., (1978), Vogels Text Book of Practical Organic Chemistry, 4th Edition, 1984 Reprint LRS/Londons, England, 280


  • There are currently no refbacks.




About ASRJETS | Privacy PolicyTerms & Conditions | Contact Us | DisclaimerFAQs 

ASRJETS is published by (GSSRR).