Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds
Keywords:
Antifungal activity, chilli seeds germination, Fusarium spp, Lactic Acid Bacteria, Rhizospheric soil.Abstract
Lactic acid bacteria (LAB) are known to inhibit the growth of fungi and LAB cells or its can be used as biocontrol against plant pathogens. The aim of this study was to evaluate the efficacy of selected LAB strains isolated from rhizospheric soil against Fusarium spp. These fungi were isolated from infected different plant parts and identified as F. oxysporum strain KAML01, Fusarium sp. CID124, Fusarium sp. fus 124 and F. proliferatum with 99 % similarity using Internal Transcribed Spacer (ITS) analysis. Five LAB strains were showed strong antifungal activity against the Fusarium spp. were evaluated using dual overlay method. Isolates MSS1 and FCF11 were 100% similarity with Lactobacillus plantarum and isolate MSS5 99% was similarity with Pediococcus pentoceous using 16S rDNA sequence analysis. Inhibition of Fusarium sp. CID124 fungal biomass in the de Man, Rogosa and Sharpe Broth (MRSB) was significantly greater (P ? 0.05) by cells of P. pentosaceus (97.43%) compared to inhibition of Fusarium sp. CID124 fungal biomass by Cell Free Supernatant (CFS) of Lb.
acidophilus ATCC314 (91.8%) using biomass dry method in Malt Extract Broth (MEB) medium. Fusarium spp. infected to seeds of three cultivars, Cili Kulai, Red spicy-0639 and GAAC-SEED-5005 and then, treated with CFS-LAB. All treated seeds with CFS-LAB showed good germination in the range of 82.6 to 93.3% compared to seed germination 53.3 to 70.0% of chili seeds infected with Fusarium spp. The percentage germination of chilli seeds was strongly suppressed when infected with Fusarium sp. CID124. This infection had significant (P?0.05) reduction of seed germination to 53.3 % compared to other Fusarium spp. Finally, the pathogenicity effect of Fusarium sp. CID124-CS was highly virulent on seeds germination of chilli.
References
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[43] B. J. A. Muhialdin, Z. Hassan, F. A. Bakar, L. H. Algboory, N. Saari, “Novel antifungal Peptides produced by Leuconostoc mesenteroides DU15 effectively inhibit growth of A. niger”,Journal of Food Science, Vol. 5, pp. 26-30, 2015.
[44] El- W. Mabrouk,“Lactic acid bacteria as biological control against Colletotrichumgloes porioides and Colletotrichum capsici on chilli plants”, Thesis. Unsersity Sains Islam Malaysia, 2014.
[45] W. El-Mobrouk, H. Zaiton, Ahmed M. M., K. M.A. H. & K. Sh. B. A.K. Fredy, “Screening of lactic acid bacteria as bio-control against (Colletotrichum capsici) on chilli bangi”, Research Journal of Biological Science, Vol. 7, No. 4, pp. 159-164., 2012.
[46] V. Jalonder and B. D. Gachonde, “Effect of cutture filtrates of Fusarium oxysporum on seed germination and seedling growth of pigeon pea (cnanus cnani varieties)”, Bioscience Discovery, Vol. 2, pp. 185-188, 2011.
[47] A. Laitila, H. L. Alakomi, L. Raaska, T. Mattila- Sandholm & A. Haikara, “Antifungal activity of two lactobacillus plantarum strains against Fusarium mould in vitro and in malting of barley”. Journal of Applied Microbiology, Vol 93, pp. 566-576, 2002.
[48] G. Newcombe, A. Shipunov, S. D. Eigenbrode, K. H. Anil Raghavendra, H. Ding, Cort L. Anderson, “Endophytes influence protection and growth of an invasive plant”, Communicative & Integrative Biology, Vol. 2, No. 1, pp. 29-31, 2009.
[2] K. Singh, J. C. Frisvad, U, Thrane and S. B. Mathur, “An iltustroted Manual on identification of Some Seed- Borne Aspergilli, Fusario, Pencillia ond their Mycotoxins”, Aio rryk as odense, Denmark, pp. 133, 1991.
[3] A.B. Anasol, P.D. & A.M. Emechebe, “Preliminary studies on toxic metabolites produced by Drechslero rostroto and Fusarium equiseti and their effect on root growth in wheat”, Nigerian Journal plant protection Vol. 5, pp. 102-105, 1981.
[4] V. Jalonder & B. D. Gachonde, “Effect of culture filtrates of Fusarium oxysporum on seed germination and seedling growth of pigeon pea (cnanus cnani varieties)”, Bioscience Discovery, Vol. 2, No. 2, pp.185-188, 2011.
[5] M. Z. Alam, I. Hamim, M. A. Ali, and M. Ashrafuzzaman, “Effect of seed treatment on seedling health of chili”, Journal of Environmental Scienc & Natural Resources, Vol. 7, No. 1, pp. 177 – 181, 2014.
[6] E. Chigoziri and E. J. Ekefan, “Seed borne fungi of chilli pepper (Capsicum frutescens) from pepper producing areas of Benue State”, Nigeria Agriculture and Biology Journal of North America ISSN Print: 2151-7517, ISSN Online: 2151-7525, 2013.
[7] M. N. Asalmol, V.P. Kale & S.T. Ingle, “Seed borne fungi of chilli, incidence and effect on seed germination”, Seed Resources, Vol. 29, pp. 76-79, 2001.
[8] R. Tharsika and G. Mikunthan, “Impact of Aspergillus fungi on germination of chilli seeds and its control”, International Journal of Scientific & Technology Research, Vol. 4, No. 05, MAY, 2015.
[9] C. Stephane, D. Brion, N. Jerzy, C. Christophe and A. Essaid, “Use of plant growth-promoting bacteria for bio-control of plant diseases: principles, mechanism of action, and future prospects”, Applied Environmental Microbiology,Vol.71,pp.4951-4959,2005.
[10] Rosalia,TLluis, B. Emilio and M. B. Esther, “Lactic acid bacteria from fresh fruit and vegetables as bio-control agents of phytopathogenic bacteria and fungi”,International Microbiology,Vol.11,pp.231-236,2008.
[11] H. Ashgar, & Mohammad, “A review on biological control of fungal plant pathogens using microbial antagonists”, Journal of Biological Science, Vol. 10, pp. 273-290, 2010.
[12] H. K. Wang, H. Yan, J. Shin, L. Huang, H. P. Zhang and W. Qi, “Activity against plant pathogenic fungi of Lactobacillus Plantarum IMAU10014 isolation from xinjiang koumiss in China”. Annals of Microbiology”, Vol.61,pp.879-885,2011.
[13] B. J. A Muhialdin, Z. Hassan & S. Sadon Kh, “Antifungal Activity of Lactobacillus fermentum Te007, Pediococcus pentosaceus Te010, Lactobacillus pentosus G004, and L. paracasi D5 on Selected Foods”, Journal of Food Science, Vol. 76, pp. 493–499, 2011.
[14] M. Plockova, J. Stiles & J. Chumchalova, “Control of mould growth by Lactobacillus rhamnosus VT1 and Lactobacillus reuteri CCM 3625 on milk agar plates”, Czech Journal of Food Science, Vol. 19, pp. 46-50, 2001.
[15] I. H. Yousef, and B. B. Lloyd, “Antifungal activity of Lactobacillus paracasei ssp. Tolerans isolated from a sourdough bread culture”, International Journal of Food Microbiology,Vol. 121. pp. 112-115,2008.
[16] El-Mabrouk, H. Zaiton, M. M. Ahmed & M. A. H. Khaled, “Screening of Lactic Acid Bacteria as Biocontrol Against (Collectotrichum capsici) on Chilli Bangi”, Research Journal of Applied Sciences, Vol.7, No. 9 & 12, pp. 446- 473, 2012.
[17] H.M. Gebreel, A.A. El-Mehalawy, I .M El-Kholy, H.M Rifaat,. and A.A. Humid, “Antimicrobial activities of certain bacteria isolated from Egyptian soil against pathogenic Fungi”, Research Journal of Agriculture and Biological Sciences, Vol. 4, No. 4, pp. 331-339, 2008.
[18] N. Noordiana, A. B. Fatimah & A. S. Mun, “Antibacterial agents produced by lactic acid bacteria isolated from Threadfin Salmon and Grass Shrimp”, International Food Research Journal Vol. 20, No. 1, pp. 117-124, 2013.
[19] J. Kohl, J. Postma, P. Nicot, M. Ruocco & B. Blum, “Stepwise screening of microorganisms for commercial use in biological control of plant-pathogenic fungi and bacteria”, Biological Control, Vol. 57, pp.1?12, 2011.
[20] P. L. Matthias, V. M. C. M.., C, C, “Lactic acid bacteria as bio-control agents of soil-borne pathogens, Biological Control of Fungal and Bacterial Plant Pathogens”, IOBC-WPRS Bulletin, Vol. 78, pp. 285-288, 2012.
[21] D. K. Jha, “Laboratory Manual on Seed Pathology”, Vikas Publishing House Private Limited, pp. 13-30, 1995.
[22] L. J. Judith, G. Anderson, R. D. Thomasd & K. S. Oliver, “Inoculation and growth with soil borne pathogenic fungi Medicago truncatula handbook version”. Australian Centre of Necrotrophic Fungal Pathogens, SABC, Department of Health Sciences, Murdoch University, South State, Murdoch 6150, Western Australia, 2006.
[23] M. Ignjatov, D. Milosevic, Z. Nikolic, J. Gvozdanovic-Varga, D. Jovicic and G. Zdjelar, “Fusarium oxysporum as Causal Agent of Tomato Wilt and Fruit Rot”, Pestic Phytomed Belgrade, Vol. 27, pp. 25–31, 2012.
[24] D.M. Geiser, M.D. Jimenez-Gasco, S.C. Kang, I. Makalowska, N. Veeraraghavan, T.J. Ward, N. Zhang, G.A. Kuldau, and K. O’Donnell, “Fusarium-ID V. 1.0: A DNA sequence database for identifying Fusarium”, European Journal of Plant Pathology, Vol. 110, pp. 473-479, 2004.
[25] F.S. Altschul, T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller & J. L. David, “Gapped BLAST and PSLBLAST: a new generation of protein database search programs”, .Nucleic Acids Research, Vol. 25, pp. 3389-3402, 1997.
[26] De Man, J.D., M. Rogosa & M.E. Sharpe,“A medium for the cultivation of lactobacilli”, Journal of Applied Bacteriology, Vol. 23, pp. 130-135. DOI: 10.1111/j.1365-2672.tb00188, 1960.
[27] P. Wanchai, S. Pramuan and W. Nisakorn, “Identification and evaluation of lactic acid bacteria for Pla-som (fermented fish) starter”, Proceedings of the 2nd International Conference on Fermentation Technology for Value Added Agriculture Products, May, 2007, pp. 23-25, Kosa Hotel, Khon Kean, Thailand. pp.1-6, 2007.
[28] B. W. Jarvis & L. M. Hoffman, “Epi-centre forum”, Vol. 11, pp. 0-5, 2004.
[29] J. Magnusson, “Antifungal activity of lactic acid bacteria”, Ph.D. Thesis, Agraria 397, Swedish University of Agricultural Sciences, Uppsala, Sweden, 2003.
[30] J. Magnusson & J. Schnurer, “Lactobacillus coryniformis subsp. coryniformis strain Si3 produces a broad-spectrum proteinaceous antifungal compound”, Applied Environmental Microbiology. Vol. 67, pp. 1-5. DOI: 10.1128/AEM.67.1.1-5, 2001.
[31] H. Hamed, A. M. Yomna and M. A. A. Shadia, “In vivo Efficacy of Lactic Acid Bacteria in Biological Control against Fusarium oxysporum for Protection of Tomato Plant”, Life Science Journal, Vol. 8, No. 4, pp. 462-468, 2011.
[32] B. J. A. Muhialdin, Z. Hassan, “Screening of Lactic Acid Bacteria for Antifungal Activity against Aspergillus oryzae”, American Journal of Applied Sciences, Vol. 8, No. 5, pp. 447-451, ISSN 1546-9239, 2011.
[33] M. N. Khare, “Methods to test seeds for associated fungi”, Indian Phyto-pathology, Vol. 49, pp. 319-328, 1996.
[34] D. Nirmaladevi1, M. Venkataramana, R. K. Srivastava, S. R. Uppalapati, V. K. Gupta, T. Yli-Mattila, K. M. Clement T., C. Srinivas1, S. R. Niranjana & N. S. Chandra, “Molecular phylogeny, pathogenicity and toxigenicity of Fusarium oxysporum f. sp. Lycopersici”, Scientific Report Vol. 6, 21367, 2012.
[35] Dhoro, M. 2010. “Identificaation and differentiation of Fusarium species using selected molecular methods”. Thesis. p. 1-74.
[36] H. Yu, Z. Lei, L. Lin, Z. Chengjian, G. Lei, L. S. Wenchao, Peixin, Q. Luping, “Recent developments and future prospects of antimicrobial metabolites produced by endophytes”, Microbiological Research. Vol. 165, pp.437-449, 2010.
[37] Sever z., d. ivic, T. kos, and T. milicevic, “Identification of Fusarium species isolated from stored apple fruit in Croatia”,Arh Hig Rada Toksikol, Vol.63, pp. 463-47, 2012.
[38] A. Ponce, M. Moreira, C. A. Del Valle & S. Roura, “Preliminary characterization of bacteriocin-like substances from lactic acid bacteria isolated from organic leafy vegetables”, LWT - Food Science and Technology, Vol. 41, No. 3, pp. 432-441, 2008.
[39] C. Ibarguren, M. Audisio, T. Farfaan, and M. Apella, “Silicates characterization as potential bacteriocins carriers”, Innovative Food Science and Emerging Technologies, Vol. 11, No.1. pp.197-202, 2010.
[40] El- W. Mabrouk Asma Saleh, Z. Hassan, A. Mahir Mokhtar Khaled. M. A. Hussin, “Antifungal Activity of Lactobacillus plantarum LAB-C5 and LAB-G7 Isolated from Malaysian Fruits”, Acta Biologica Malaysiana, Vol. 2, No. 1, pp. 22-30, 2013.
[41] Y.S. Chen, F. Yanagida & T. Shinohara, “Isolation and identification of lactic acid bacteria from soil using an enrichment procedure”, Letters in Applied Microbiology, Vol.40, pp.195–200, 2005.
[42] B. J. A. Muhialdin, Z. Hassan, F. A. Bakar, L. H. Algboory, N. Saari, “Identification of antifungal peptides produced by Lactobacillus plantarum IS10 grown in the MRS broth”, Food Control, Vol. 59, pp. 27, 2016.
[43] B. J. A. Muhialdin, Z. Hassan, F. A. Bakar, L. H. Algboory, N. Saari, “Novel antifungal Peptides produced by Leuconostoc mesenteroides DU15 effectively inhibit growth of A. niger”,Journal of Food Science, Vol. 5, pp. 26-30, 2015.
[44] El- W. Mabrouk,“Lactic acid bacteria as biological control against Colletotrichumgloes porioides and Colletotrichum capsici on chilli plants”, Thesis. Unsersity Sains Islam Malaysia, 2014.
[45] W. El-Mobrouk, H. Zaiton, Ahmed M. M., K. M.A. H. & K. Sh. B. A.K. Fredy, “Screening of lactic acid bacteria as bio-control against (Colletotrichum capsici) on chilli bangi”, Research Journal of Biological Science, Vol. 7, No. 4, pp. 159-164., 2012.
[46] V. Jalonder and B. D. Gachonde, “Effect of cutture filtrates of Fusarium oxysporum on seed germination and seedling growth of pigeon pea (cnanus cnani varieties)”, Bioscience Discovery, Vol. 2, pp. 185-188, 2011.
[47] A. Laitila, H. L. Alakomi, L. Raaska, T. Mattila- Sandholm & A. Haikara, “Antifungal activity of two lactobacillus plantarum strains against Fusarium mould in vitro and in malting of barley”. Journal of Applied Microbiology, Vol 93, pp. 566-576, 2002.
[48] G. Newcombe, A. Shipunov, S. D. Eigenbrode, K. H. Anil Raghavendra, H. Ding, Cort L. Anderson, “Endophytes influence protection and growth of an invasive plant”, Communicative & Integrative Biology, Vol. 2, No. 1, pp. 29-31, 2009.
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2017-03-24
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Husain, A., Hassan, Z., Huda-Faujan, N., & Lani, M. N. (2017). Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds. American Scientific Research Journal for Engineering, Technology, and Sciences, 29(1), 182–202. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/2760
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