Study of Antibiotic Resistivity of Some Bacteria from Selected Fish Species of Patna
Keywords:
Antibiotics, Bacteria, Fish, Pathogen, ResistivityAbstract
Infection caused by pathogens transmitted to humans by various means and fish is one of them. Fish are susceptible to various microorganisms (bacterial pathogens) which is the part of the micro environment. The purpose of this study was for isolation of bacteria from fish organ and impact of generic antibiotics upon isolated bacteria for potential checking. The study conducted between October to December, 2019 in the Department of Biotechnology, by selecting three species of fish Eutropiichthys vacha (Batchwa), Ctenopharyngodon idella (Grass Carp) and Labeo rohita (Rohu) from local fish market of Patna district. Possible bacteria isolated from fish are: Proteus vulgaris, Bacillus subtilis/P. aeruginosa, Vibrio cholera, Klebsiella aerogenes, Klebsiella pneumonia, Staphylococcus aureus, and Salmonella typhi from the selected organ are gills, intestine, heart, liver and muscle. The isolated bacteria identified by using biochemical test (iMVIC) and antibiotics susceptibility test was conducted for testing impact of antibiotics on it by using 13 antibiotics were used in this work. The result of the study showing that rohu fish sold in the market has highly bacterial contamination and antibiotics resistance. We are strongly suggested the urgent need to improve the quality of fish supplied in the fish market Patna.
References
[1]. Hastein T, Hjeltnes B, Lillehaung A, Utne Skare J, Berntssen M, Lundebye Ak (2006) Food Safety hazards that occur during the production stage: challenges for fish farming and the fishing industry. Rev Sci Technol 25(2):607-625
[2]. Alexopoulos A, Plessas S, Voidarou C, Noussias H, Stavrpoulou E, et al. (2011) Microbial ecology of fish species on growing in Greek sea farms and their watery environment. Anaerobe 17:264-266.
[3]. Al-Harbi AH, Uddin N (2005) Bacterial diversity of tilapia (Oreochromis niloticus) cultured in brackish water in Saudi Arabia. Aquaculture 250:566-572.
[4]. Austine B (1983) Bacterial microflora associated with a coastal, marine fish-rearing unit. Journal of Marine Biological association of the United Kingdom 63:585-592.
[5]. Kvenberg EJ. In: Donn R, Cameron H, Van Nostrand R, editors. Non-Indigenous Bacterial Pathogen. New York: Microbiology of Marine Food Products;(1991). P. 263-91.
[6]. ICMSF (International Commission for Microbiological Specification for Foods) (1998) Microorganisms in foods 6. Blackie Academic, London.
[7]. Das, M.F., hafiz, M.K. Ahmed and S. Parveen, (2007). Microbiological analysis of some raw fish samples, Bangladesh. J. Microbial., 24(I):67-69.
[8]. Hinton M, Kaukas A, Linton AH. The ecology of drug resistance in enteric bacteria. Journal of applied bacteriology (1986); 15:77-92
[9]. Kanth MK*, Singh SK, Kashyap A, Gupta VK, Shalini S, Kumari S, Kumari R, Puja K. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2017) Volume 38, No 2, pp 161-167, ISSN:2313-4402.
[10]. Obi SKC, Krakowiaka A (1983) Theory and Practice of Food Microbiology (unpublished manual)
[11]. Cheesbrough MC. District laboratory practice in tropical countries. 1st Edition, Cambridge University Press, UK. 2006;115.
[12]. L. Samuel, M. M. Marian, K. Apun, M. B. Lesley, and R. Son, “Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis,” International Food Research Journal, vol. 18, no. 3, pp. 971–976, 2011.
[13]. Clinical and Laboratory Standards Institute (CLSI), Performance Standards for Antimicrobial Susceptibility Testing: Nineteenth Information Supplement, CLSI, Pennsylvania, Pa, USA, 2012
[14]. R. Bonnet, Comit´e de I’Antibiogramme de la Soci´et´eFranc¸aisede Microbiologie, CASFM, 2012.
[15]. P. H. Krumperman, “Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods,” Applied and Environmental Microbiology, vol.46, no. 1, pp. 165–170, 1983.
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