Comparative Effects of Bone Char and NPK Agricultural Fertilizers on Hydrocarbon Utilizing Bacteria and Fungi in Crude Oil Polluted Soil
Bone char and NPK fertilizers are stimulants for bioremediation of crude oil polluted soil were investigated. Cells were constructed in-situ with dimensions of 1.5m by 1.5m. Crude oil samples were applied to cells with crude penetration depths of 30 cm. The hydrocarbon content (THC), total organic carbon (TOC), bacterial and fungal contents of the soils of the cells were investigated before and 8 weeks after addition of 0.5kg, 2kg and 3.5kg of bone char and NPK fertilizer. The control cells had no bone char or NPK fertilizer. The results showed that bone char and NPK fertilizer significantly reduced THC and TOC when compared with the control. Furthermore, both bone char and NPK fertilizers significantly increased the number of hydrocarbon utilizing bacteria and fungi as well as total heterotrophic bacteria population. Consequently, THC removal efficiency ranged from 62.24 to 87.74% and TOC removal efficiency ranged from 62.93 to 77.37% for NPK fertilizer and bone char amended cells, respectively. The stimulatory efficiency for THC ranged from 82.00 to 87.23% and stimulatory efficiency for TOC ranged from 72.40 to 77.55% for NPK and bone char respectively. In conclusion, our results suggest that the stimulatory effects of bone char for bioremediation of crude oil contaminated soil are comparative with those of NPK fertilizer.
O. Lindén, J. Pålsson. “Oil contamination in Ogoniland, Niger Delta.” Ambio, vol. 42, pp. 685–701, 2013.
B. B. Babatunde, Sikoki FD, Avwiri GO, Chad-Umoreh YE. “Review of the status of radioactivity profile in the oil and gas producing areas of the Niger delta region of Nigeria.” J Environ Radioact, vol. 202, pp. 66-73, 2019.
E. S. Osuagwu, E. Olaifa. “Effects of oil spills on fish production in the Niger Delta.” PLoS One, vol. 13, no. 10, e0205114, 2018.
B. Ordinioha, S. Brisibe. “The human health implications of crude oil spills in the Niger delta, Nigeria: An interpretation of published studies.” Niger Med J, vol. 54, pp. 10–16, 2013.
O. Maduka, C. Tobin-West. “Is living in a gas-flaring host community associated with being hypertensive? Evidence from the Niger Delta region of Nigeria.” BMJ Glob Health, vol. 2, e000413, 2017. doi:10.1136/bmjgh-2017-000413.
P. C. Manyike, J. M. Chinawa, A. Ubesie, H. A. Obu, O. I. Odetunde, A. T. Chinawa. “Prevalence of malnutrition among pre-school children in, South-east Nigeria.” Ital J Pediatr, vol. 40, 75, 2014.
U. F. Ekpo, A. M. Omotayo, M. A. Dipeolu. “Prevalence of malnutrition among settled pastoral Fulani children in Southwest Nigeria.” BMC Res Notes, vol. 1, 7, 2008.
World Health Organization (WHO). WHO data set on child malnutrition. Geneva, WHO Press, 2014.
U. M. Obiakalaije, O. A. Makinde, E. R. Amakoromo. “Bioremediation of crude oil polluted soil using animal waste.” International Journal of Environmental Bioremediation & Biodegradation, vol. 3, no. 3, pp. 79-85, 2015.
T. P. Nwogu, C. C. Azubuike, C. J. Ogugbue. “Enhanced bioremediation of soil artificially contaminated with petroleum hydrocarbons after amendment with Capra aegagrus hircus (Goat) Manure.” Biotechnol Res Int, vol. 2015, 657349, 2015. doi: 10.1155/2015/657349.
V. B. Khodse, N. P. Lotlikar, R. M. Meena, S. R. Damare. “Bioremediation potential of hydrocarbon-utilizing fungi from select marine niches of INatasha Maria Barnes.” 3 Biotech, vol. 8, 21, 2018.
I. F. H. A. Jawhari. “Ability of some soil fungi in biodegradation of petroleum hydrocarbon.” Journal of Applied & Environmental Microbiology, vol. 2, no. 2, pp. 46-52, 2014.
C. E. Anih, A. Okewale, N.-O. E. Moses. Effect of nutrients on bioremediation of crude oil-polluted water. American Journal of Environmental Science and Engineering, vol. 3, pp. 1-7, 2019.
K. C. Ubochi, V. I. Ibekwe, E. U. Ezeji. “Effect of inorganic fertilizer on microbial utilization of hydrocarbons on oil contaminated soil.” African Journal of Biotechnology, vol. 5, no. 17, pp. 1584-1587, 2006.
M. Kalita, A. Devi. “Study on the effects of soil ph and addition of n-p-k fertilizer on degradation of petroleum hydrocarbon present in oil-contaminated soil.” International Journal of Chemical and Petrochemical Technology (IJCPT), vol. 2, no. 3, pp. 9-22, 2012.
J. Rawat, J. Saxena, P. Sanwal. “Biochar: a sustainable approach for improving plant growth and soil properties.” London, United Kingdom: IntechOpen, 2019. doi: 10.5772/intechopen.82151
J. Lehmann, M. C. Rillig, J. Thies, C. A. Masiello, W. C. Hockaday, D. Crowley. “Biochar effects on soil biota – A review.” Soil Biology and Biochemistry, vol. 43, no. 9, pp. 1812-1836, 2011.
A. Walkley, I. A. Black. “An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromicacid titration method.” Soil Science, vol. 37, pp. 29–38, 1934.
R. H. Bray, L. T. Kurtz. “Determination of total, organic, and available forms of phosphorus in soils”. Soil Science, vol. 59, pp. 39-45, 1945.
C. T. Odu, O. F. Esuruoso, L. C. Nwoboshi, J. A. Ogunwale. “Environmental study of the Nigerian Agip Oil Company operational areas.” Proceedings of the soils and fresh water vegetation conference, Milan, Italy, 1985.
G. J. Bouyoucos. “Hydrometer method improved for making particle size analysis of soils.” Agron J, vol. 54, pp. 464-465, 1962.
G. J. Bouyoucos . “Directions for Making Mechanical Analysis of Soils by the Hydrometer Method.” Soil Science, vol. 4, pp. 225–228, 1936.
R. P. Day. “Pipette method of particle size analysis. In: Methods of soil analysis.” Agronomy, vol. 9, pp. 553-562, 1965.
R. E. Buchanan, N. E. Gibbons, eds. Bergey’s Manual of Determinative Bacteriology, 8th edn. Baltimore, MD, USA: Baltimore, Williams and Wilkins Co., 1974.
S. T. Cowan. “Cowan and Steel’s Manual for the Identification of Medical Bacteria, 2nd edn. Cambridge, London: Cambridge University Press, 1974.
W. F. Harrigan, M. E. McCane. Laboratory Methods in Food and Diary Microbiology. 8th ed. London: Academic Press, 1990.
T. F. Khan, M. M. Ahmed, S. M. Imamul Huq. “Effects of biochar on the abundance of three agriculturally important soil bacteria.” Journal of Agricultural Chemistry and Environment, vol. 3, no. 2, pp. 31-39, 2014.
S. D. Umboh, C. L. Salaki, M. Tulung, L. C. Mandey, R. T. D. Maramis. “The isolation and identification of fungi from the soil in gardens of cabbage were contaminated with pesticide residues in Subdistrict Modoinding.” International Journal of Research in Engineering and Science, vol. 4, no. 7, pp. 25-32, 2016.
C. Ditzler, K. Scheffe, H. C. Monger, eds. Soil survey manual. USDA Handbook 18. Washington, D.C: Government Printing Office, 2017.
L. O. Odokuma, A. A. Dickson. “Bioremediation of a crude oil polluted tropical mangrove environment.” Global Journal of Applied Sciences and Environment, vol. 7, no. 2, pp. 23-29, 2003
L. O. Odokuma, A. A. Dickson. “Bioremediation of a crude oil polluted tropical rainforest soil.” Global of Applied Sciences and Environment, vol. 2, no. 1, pp. 29-40, 2003.
N. Ali, M. Eliyas, H. Al-Sarawi, S. S. Radwan. “Hydrocarbon-utilizing microorganisms naturally associated with sawdust.” Chemosphere, vol. 83, no. 9, pp.1268-1272, 2011.
S. Schneiker, A. P. V. Martins dos Santos, D. Bartels, T. Bekel, M. Brecht, J. Buhrmester, et al. “Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis.” Nature Biotechnology, vol. 24, pp. 997–1004, 2006.
D. J. Naether, S. Slawtschew, S. Stasik, M. Engel, M. Olzog, L. Y. Wick, K. N. Timmis, H. J. Heipieper. “Adaptation of the hydrocarbonoclastic bacterium Alcanivorax borkumensis SK2 to alkanes and toxic organic compounds: a physiological and transcriptomic approach.” Appl Environ Microbiol, vol. 79, no. 14, pp. 4282–4293, 2013.
J. S. Sabirova, M. Ferrer, D. Regenhardt, K. N. Timmis, P. N. Golyshin. “Proteomic insights into metabolic adaptations in Alcanivorax borkumensis induced by alkane utilization.” J Bacteriol, vol. 188, no. 11, pp. 3763–3773, 2006.
D. W. Graham, V. H. Smith, D. L. Cleland, K. P. Law. “Effects of nitrogen and phosphorus supply on hexadecane biodegradation in soil systems.” Water Air and Soil Pollution, vol. 111, pp. 1-18, 1999.
H. Al-Awadhi, H. Al-Awadhi, D. Al-Mailem, N. Dashti, L. Hakam, M. Eliyas, S. Radwan. “The abundant occurance of hydrocarbon-utilizing bacteria in the phyllospheres of cultivated and wild plants in Kuwait.” International Biodeterioration & Biodegradation, vol. 73, pp. 73-79, 2012.
A. A. Adekunle, O. A. Adebambo. “Petroleum hydrocarbon utilization by fungi isolated from Detarium Senegalense (J. F Gmelin) Seeds.” Journal of American Science, vol. 3, pp. 69-76, 2007.
C. B. Chikere, C. C. Azubuike. “Characterization of hydrocarbon utilizing fungi from hydrocarbon polluted sediments and water.” Nig J Biotech, vol. 27, pp. 49–54, 2014.
R. U. Ofoegbu, Y. O. L. Momoh, I. L. Nwaogazie. “Bioremediation of crude oil contaminated soil using organic and inorganic fertilizers.” J Pet Environ Biotechnol, vol. 6, pp. 198, 2014.
S. E. Agarry, G. K. Latinwo. “Kinetic modelling of bioremediation of water contaminated with Bonny Light crude oil using biostimulation-bioaugmentation.” Universal Journal of Environmental Research and Technology, vol. 5, no. 4, pp. 188-200, 2015.
- There are currently no refbacks.