The Effects of Different Concentrations of Sliver Nanoparticles on the Kidneys of Male Albino Mice

Reham Yousef Abdul Rahman Zamil

Abstract


Silver nanoparticles (AgNPs) are one of the most abundantly used nanomaterials. Following exposure, AgNPs can accumulate in various organs including kidneys. This study aimed to determine the effects of increased doses of AgNPs on the kidneys of adult male albino mice. Sixty adult white male mice (20-25 g) were randomized divided equally into four groups (15 mice each): control group (saline solution), low-dose group (LD AgNPs) was intraperitoneal (i.p.) injected with 1 g/ kg, medium dose group (MD AgNPs) was i.p. injected with a dose of 2 g/ kg and high dose group (HD AgNPs) was i.p. injected with a dose of 4 g/ kg. Samples were taken from each group after one week, two weeks and four weeks of injection. Then blood was collected from all animals for serum analysis of kidney functions (serum creatinine, uric acid and blood urea nitrogen) at the end of each period and the kidneys were examined microscopically. The results showed impaired kidney function as revealed by increased in sera levels of creatinine, uric acid and blood urea nitrogen in rats treated with AgNPs. The study of light microscopy showed tissue changes in the kidney membranes including programmed death and focal necrosis. One of the most significant effects was changes in nuclei that appeared either enlarged or bilateral in the kidney cells. The biochemical and structure were more prominent by increased dose and prolonged duration. The study concluded that despite the benefits of silver nanoparticles, it is important to test the safety of the use of different doses to determine the safe dose, especially on vital organs such as kidneys.


Keywords


Silver nanoparticles; mice; kidneys; nephrotoxicity; structure.

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