Extraction and Characterization of Linoleic Acid from the Leaves of the Traditional Medicinal Plant Caloncoba Echinata in Sierra Leone

Lahai Koroma, T. B. R. Yormah, L. M. Kamara, G. M.T. Robert

Abstract


Dried powdered organs of Caloncoba echinata plant were subjected to organoleptic evaluation and Fluorescence properties. The reagent which gave the most fluorescent character was used to extract a compound from the plant materials. 3.14% (2.50 g) of an oily substance was extracted from 79.62g of powdered leaves of Caloncoba echinata in 450 mL 10% of HNO3 and allowed to stand for 72 hours. The extract was filtered using a Buchner funnel attached to portable Vacuum Pump and the acidic crude extracted with petroleum ether (10 mL x 3).The crude oily compound was separated from the mixture, purified weighed and labelled as LKL01. Sample LK01 tested positive for terpenoids and unsaturation with Saponification and Iodine Values of 201.96 and 177.66 respectively indicating that the compound is very suitable soap production and cosmetic purposes. Chemical and spectroscopic analysis and from literature revealed the compound to be Linoleic acid. This is the first report of the presence of Linoleic acid in the leaves of Caloncoba echinata. Linoleic acid has been reported to be used as an emollient and thickening agent in cosmetics, antioxidant and an anti-inflammatory agent in the treatment of burns, cold sores and other minor wounds supporting the use of Caloncoba echinata plant in traditional medicine.


Keywords


Linoleic Acid; Organoleptic; Fluorescence; Pharmacognostical evaluation; Saponification value; Iodine value and McLafferty Rearrangement.

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References


Hanson, S.W., 1977. Local plants of medicinal interest. Part 4: Habropetalum dawei. Chemistry in Sierra Leone 4: 38–40

Hanson, S.W., Crawford, M. & Thanasingh, D.P.J., 1981. (+)-Isoshinanolone and 2 methylbenzofuran-4-carbaldehyde from the fish-stunning plant Habropetalum dawei. Phytochemistry 20: 1162.

Gagan Shah, Richa Shri, Vivek Panchal,2 Narender Sharma, Bharpur Singh, and A. S. Mann (2011) Scientific basis for the therapeutic use of Cymbopogon citratus, stapf (Lemon grass) J Adv Pharm Technol Res. 2011 Jan-Mar; 2(1): 3–8

Basil Nse Ita, Lahai Koroma and Kalilu Kormoh, Isolation and characterization of inositol from the ethanolic leaf extract of Aspilia africana. J. Chem. Pharm. Res., 2010, 2(4):1-6.

Ayensu, E. S. Medicinal Plants of West Indies, Ed. 1, 32; Michigan; Reference publication Inc 1981

Sofowora, E. A. and Odebiyi, O. O. Lloydia, 1978, 41, 234 – 246

Ziegler HL, Staerk D, Christensen J, Olsen CE, Sittie AA, Jaroszewski JW. New dammarane and malabaricane triterpenes from Caloncoba echinata J Nat Prod. 2002 Dec; 65(12):1764-8.

Ziegler H. L. Ziegler and coauthors, Royal Danish School of Pharmacy, 2003

Burkill, H. M. The useful plants of west tropical Africa. (Use Pl WT Afr) Chapter 13. sinaver Association, sunder land, 1985

Burkill, H. M. The useful plants of west tropical Africa. (Use Pl WT Afr) Chapter 13. sinaver Association, Sunder land, 2004

Keay, R. W. J. & F. N. Hepper. Flora of west tropical, 1953

Keay, R. W. J. & F. N. Hepper. Flora of west tropical, 1972

Mabberley, D. J. The plant-book: a portable dictionary of the vascular plants, ed. 2. 1997. (Pl Book).

Bouquet A., Debray M. (1974) Plantes médicinales de la Côte-d'Ivoire, O.R.S.T.O.M., p. 232 France

Siddiqui, Hakim MA. Format for the pharmacopoeia analytical standards of compound formulation, workshop on standardization of Unani drugs, (appendix), 24‐25 January. New Delhi: Central Council for Research in Unani Medicine (CCRUM); 1995.

Chase CR, Pratt RJ: Fluorescence of powdered vegetable drugs with particular reference to development of a system of identification. J. Amr. Pharm. Assoc., 1949; 38: 324‐ 331.

Haryati T., Che Y.B. Man, Ghazali H. M., Asbi B.A., Buana L.(1998): Determination of iodine value of palm oil based on triglyceride composition. J. of the American oil Chemist’s society, 1998, volume 75, Issue 7 pp 789 - 792

JIS K 0070 – 1992 Test method for acidity, Saponification value, Ester value, Iodine value and Hydroxy value of Chemical products and saponifiables

David T Plummer (1987) An Introduction to Practical Biochemistry, third edition by, McGraw-Hill, C. McGraw-Hill Book Compny (U.K.) Ltd, London. 1987

Vlab.amrita.edu. (2011) Estimation of Saponification Value of Fats/Oils

ISO 3657:2002 Animal and vegetable fats and oils – Determination of saponification value

Firestone D. (1994) “Determination of the iodine value of oils and fats: summary of collaborative study” J AOAC Int. 77 (3); 674-6. 1994

Thomas, Alfred (2002). “Fats and fatty oils” Ullmann’s Encyclopedia of Industrial Chemistry. Weinheim: Wiley-VCH

Morio Yasuda, 1931; The determination of the iodine number of lipids 1931, 94:401-409.

Siddiqui, Hakim MA. Format for the pharmacopoeia analytical standards of compound formulation, workshop on standardization of Unani drugs, (appendix), 24‐25 January. New Delhi: Central Council for Research in Unani Medicine (CCRUM); 1995.

Chase CR, Pratt RJ: Fluorescence of powdered vegetable drugs with particular reference to development of a system of identification. J. Amr. Pharm. Assoc., 1949; 38: 324‐ 331

Ansari S.H Essentials of Pharmacognosy. 1st Edn. New Delhi; Birla PublicationsPvt. Ltd.; 2006

Morio Yasuda, 1931; The determination of the iodine number of lipids 1931, 94:401-409.

Furst, A; Pretsch, W; Robien, W, “A comprehensive parameter set for the prediction of the 13C NMR chemical shifts of sp3 – hybridized carbon atoms in organic compounds, Anal. Chim. Acta 1990, 213, 233

Marshall, J.L., Carbon – Carbon and Carbon – Proton NMR couplings, Verlag Chemie International, Deerfield Beach, FL, 1983

Gerhard Knothe and James A. Kenar: Determination of the fatty acid profile by 1H-NMR spectroscopy, Eur. J. Lipid Sci. Technol. 106 (2004) 88–96

Ahmad, I., Suits, F., Hoekman, B., Swertz, M. A., Byelas, H., Dijkstra, M., Hooft, R., Katsubo, D., van Breukelen, B., Bischoff, R. & Horvatovich, P. (2011). A high-throughput processing service for retention time alignment of complex proteomics and metabolomics LC-MS data, Bioinformatics 27(8): 1176–1178.

Christie, W.W. (2000b) WWW home page of Lipid Analysis Unit, Mylnefield Research Services, Dundee, Scotland. < http://www.lipid.co.uk/>

Christie, W.W., Rebello, D., Holman, R.T.: Mass spectrometry of derivatives of cyclopentenyl fatty acids. Lipids 4, 229–231 (1969)

Ando H, Ryu A, Hashimoto A, Oka M, Ichihashi M. Linoleic acid and alpha-linolenic acid lightens ultraviolet-induced hyperpigmentation of the skin. Arch Dermatol Res. 1998; 290(7):375-81.

Letawe C, Boone and M, Piérard G. Digital image analysis of the effect of topically applied linoleic acid on acne microcomedones. Clin Exp Dermatol. 1998; 23(2):56-8.

Valdman-Grinshpoun Y, Ben-Amitai D, Zvulunov A. Barrier-Restoring Therapies in Atopic Dermatitis: Current Approaches and Future Perspectives. Dermatol Res Pract. 2012; 2012:923134.

Burr G. O., Burr M. M;. On the nature and role of the fatty acids essential in nutrition. Journal of Biological Chemistry. 1930;86:587–621.

Akoh C.C. and Min D.B. “Food lipids: chemistry, nutrition, and biotechnology” 3th ed. 2008

Chow Ching K. “Fatty acids in foods and their health implication” 3th ed. 2008

Dr. Duke's Phytochemical and Ethnobotanical Databases. Plants with a chosen chemical. Linoleic Acid. Washington, DC: US Dept Agric, Agric Res Service. Available from, as of Jan 17, 2014:


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