Evaluation of the Antibacterial Activity of Chromolaena Odorata in Wistar Rats and its Chemical Characterization

Joshua Isirima Charles, Iyeopu Siminialayi Minakiri


Chromolaena odorata is a plant that has been used traditionally in the treatment of diverse disease conditions. This study sort to evaluate the anti-Salmonella typhi potential of Chromolaena odorata compared to ciprofloxacin in Wistar rats and to identify the phytochemical constituents present in the plant that could be responsible for the antibacterial activity exhibited by the plant.  The animals were divided into six groups: group 1 was normal control, group 2 was infected with Salmonella typhi without treatment, groups 3, 4 and 5 were Salmonella typhi infected and treated with 100mg/kg, 200mg/kg and 400mg/kg of the extract respectively and group 6 was infected and treated with 500mg/70kg of ciprofloxacin. The animals were inoculated with a single infective dose of Salmonella typhi bacteria and subsequently, treated with the graded doses of methanol leaf extract of Chromolaena odorata and 500mg/70kg dose of ciprofloxacin for a period of fifteen days, when the animals were confirmed infected. Fecal matter was collected from each group on day 0 (when the animals were confirmed infected) and on every five days interval for the period of the study, for Salmonella typhi load assay. This was followed by phytochemical screening and GC-MS analysis of the plant extract. Treatment of infected animals with the extract and standard drug, resulted in a dose and time dependent eradication of the Salmonella typhi from the stool, while the phytochemical screening and the GC-MS analysis revealed the presence of phenolic, flavonoid, alkaloid, steroid and Geijerene,         3-methyl-3-vinyl- cyclopropene;      4, 6-Dimethyl-3-nitro-2(1H)-pyridinone, 2-Hexyn-1-ol,                1,2,3,4-Tetrahydro-2,3-dimethylquinoxaline, nonyl prop-1-en-2-yl ester Carbonic acid respectively. Thus the observed antibacterial activity could be attributed to the presence of these phytochemicals.


Chromolaena odorata; Phytochemical Screening; GC-MS analysis; Salmonella typhi; Wistar Rats.

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Fabrega, A., & Vila, J. (2013). Salmonella enterica serovar Typhimurium skills to succeed in the host: virulence and regulation. Clinical Microbiology Reviews, 26 (2), 308-341.

Shahane, V. Muley, V. Kagal, A. Bharadwaj R. (2007). Non-typhoid Salmonellosis: Emerging infection in Pune? Indian Journal of Medical Microbiology, 25 (2):173-4 ·

Saeed, A. M., & Naji, R. (2007). Infection Agents. Epidemiologic Principles and Food Safety, Edited by Tamar Lasky Oxford University Press, 18-40.

Oksman-Caldentey, K. & Inze, D. (2004). Plant cell factories in the post-genomic era: New ways to produce designer secondary metabolites. Trend in Plant Science, 9, 433-440.

Salim, A. A., Chin, Y. W., & Kinghorn, A. D. (2008). Drug Discovery from Plants. Ramawat KG, Merillon JM (eds.), In: Bioactive Molecules and Medicinal Plants Chapter DOI: 10.1007 / 978-3-540-74603-4_1, c Springer 1-25

Gautam, R., Saklani, A., & Jachak, S. M. (2007). Indian medicinal plants as a source of antimycobacterial agents. J. Ethnopharmacol, 110, 200–234.

Thang, P. T., Patrick, S., Teik, L. S., & Yung, C. S. (2001). Anti-oxidant effects of the extracts from the leaves of Chromolaena odorata on human dermal fibroblasts and epidermal keratinocytes against hydrogen peroxide and hypoxanthine-xanthine oxidase induced damage. Burns, 27, 319-327.

Timbilla, J. A., & Braimah, H. (1994). The menacing effects of Chromolaena odorata: a case study in the Brong Ahafo region of Ghana. Third international workshop on biological control and management ofChromolaena odorata, Cote d’ Ivoire, 5.

Younes, A., Hamouda, A., & Amyes, S. G. (2011) “First Report of a Novel Extended-Spectrum Beta-Lactamase KOXY-2 Producing Klebsiella oxytoca That Hydrolyses Cefota- xime and Ceftazidime,” Journal of Chemotherapy. 23(3), 127-130.

Naidoo,K. K., Coopoosamy, R. M. and Naidoo, G.(2011). Screening of Chromolaena odorata L King and Robinson for Antibacterial and Antifungal Properties. Journal of Medicinal Plants Research 519:4839- 4862.

Hanan, B., Akram, H., Hassan, R., Ali, H., Zeinab, S., & Bassam, B. (2013). Techniques for the Extraction of Bioactive Compounds from Lebanese Urtica dioica. Maceration method. American Journal of Phytomedicine and Clinical Therapeutics, 1(6), 507-513

Watson, K. C. (1954). Clot culture in typhoid fever. J Clin Pathol, 7, 305-307.

. Watson, K. C. (1978). Laboratory and clinical investigation of recovery of Salmonella typhi from blood. J Clin Microbiol 7, 122-126.

. Taye, B., Giday, M., Animut, A., & Seid, J., (2011). Antimicrobial Activities of Selected Medicinal Plants in Traditional Treatment of Human Wounds In Ethiopia. Asian Pacific Journal of Trop Biomed, 1(5), 370-375.

. Jeyachandran, R., & Mahesh, A. (2007). Antimicrobial Evaluation of Kigelia Africana (Lam). Research Journal of Microbiology, 2(8), 645-649.

. Ngeny LC, Magiri E, Mutai C, Mwikwabe N, Bii C, Antimicrobial Properties and Toxicity of Hagenia abyssinica (Bruce) J. F. Gmel, Fuerstia africana, T. C. E. Fries, Asparagus racemosus (Willd.) and Ekebergia capensis Sparrm. African Journal of Pharmacology and Theurapeutics, 2013, 2(3): 76-82.

. Agyare, C. Anita, S. D,1 Nicholas, A.,1 Yaw, D. B.,1 Kwesi, B. M.,2 Patrick, G. A.,3 & Adarkwa-Yiadom. M. (2013). Antimicrobial, Antioxidant, and Wound Healing Properties of Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC. Advances in Pharmacological Science,

, (10).

. Adegoke, A. A. & Adebayo-tayo, B C. (2009). Antibacterial activity and phytochemical analysis of leaf extracts of Lasientheraafricanum. African Journal of Biotechnology, 8 (1), 77-80.

. Hewitt, W., & Vincent, S., (1989). In: Theory and application of microbiological assay. Academic Press, San Diego, p. 39.

. Singh, B., Sahu, P. M., & Sharma M. K., (2002). Anti-inflammatory and Antimicrobial activities of triterpenoids from Strobilanthes callosus Nees. Phytomed, 9,355-359.

. Talukdar, A. D., Choudhary, M. D., Chakraborty, M. & Dutta, .B. K: (2010). Phytochemical screening and TLC profiling of plant extracts Cyathea gigantea (Wall. Ex. Hook.) Haltt. and Cyathea brunoniana. Wall.ex.Hook. (Cl.& Bak.). Assam UniversityJournal of Science & Technology: Biological and Environmental Sciences, 5(1), 70-74.

. Mead, R. and Curnow, R. N. (1982). A simple statistical method in Agriculture and experimental Biology. Charpman Hall, London, UK, pp: 33-46.

. Akinmoladun, A. C., Ibokun, E. O., & Dan-Ologe, I. A. (2007). Phytochemical Constituents And Antioxidant Properties of Extracts From the Leaves of Chromolaena odorata. Scientific Research and Essay, 2(6), 191-194.

. Harlina., A. P., Eddy, S.& Happy, N. (2013).The Identification of Chemical Compound and Antibacterial Activity Test of Kopasanda (Chromolaena Odorata L.)Leaf Extract Against Vibriosis-Causing Vibrio harveyi (MR 275 Rif) on Tiger Shrimp Aquatic Science and Technology, 1 (2), 15-29.

. Vital P. G., & Rivera, W. L. (2009). Antimicrobial Activity and Cytotoxicity of Chromolaena odorata (L.f.) King and Robinson and Uncaria perrottetii (A. Rich) Merr. Extracts. Journal of Medicinal Plants Research, 3(7), 511-518.

. Nwinuka, N., Nwiloh, B. & Eresama, J. (2009). Nutritional and potential medicinal value of chromolaena odorata leaves. Int. J. Tropical Agric. and Food Systems, 3(2),

. Okpashi, V. E., Bayim, P. R. & Obi-Abang, M. (2014). Antimcrobial Effect of Independence Leaves (Chromolaena Odorata) Extracts. International Journal of Scientific & Engineering Research, 5(5), 949-955.

. Volk, W. A., & Wheeler. (1993). Basic Microbiology (p. 390). Miscellaneous: Markham Edition V publisher. Jakarta.

. Cowan, M. M. (1999). Plants Product as Antimicrobial agent. Clinical Microbiology review, 12(4), 564-582.

. Mori, A., Nishino, C., Enoki, N., & Tawata, S. (1987). Antibacterial Activity and Mode of action of Plant Flavonoids againt Proteus vulgaris and Staphylococcus auratus. Phytochemistry, 26, 2231-2234.

. Putra, I. N. K. (2007). Study Power Antimicrobial Preservatives Plant Extract Multiple Materials destroyer Nira Nira Against Microbes and Gynecology Actively compounds. (Unpublished Doctoral Dissertation). University of Brawijaya Malang.

. Peiman, A., Hamid, T., Masoud, R., & Amir, H. E. R. (2014). Properties and antimicrobial activity of edible methylcellulose based film incorporated with Pimpinella affinis oil. European Journal of Experimental Biology, 4(1), 670-676

. Verdian-Rizi, M. (2008). Composition and Antimicrobial Activity of Pimpinella affinis ledeb. Essential oil growing in Iran. ChemicResearch journal of biological sciences, 3 (8), 913-915.

. Wafa, T., Khaldoun, B., & Zahia, K. (2016). Comparative Compositions and Antibacterial Activity of the Essential Oils of Anthemis nobilis L. And Anthemis mixta L. (Asteraceae). International Journal of Pharmacy and Pharmaceutical Sciences, 8 (7), 456-459

. Yılmaz, Y., et al. (2011). Synthesis, microwave-promoted catalytic activity in Suzuki–Miyaura cross-coupling reactions and antimicrobial properties of novel benzimidazole salts bearing trimethylsilyl group. Applied Organometallic Chemistry, 25,366-373.

. Cong, T., et al., (2011). Synthesis and antibacterial activity of novel 4 -O-benzimidazolyl clarithromycin derivatives. European Journal of Medicinal Chemistry 2011, 46,3105-3111.

. Mónica, V., Cátia, P., Rúben, F,. João, P., & Noronhaa, C. P. (2014). Antimicrobial activity of quinoxaline 1, 4-dioxide with 2- and 3-substituted derivatives, Microbiological Research 169, 287– 293

. Blondeau, J. M., Laskowski, R., Bjarnason, J., Stewart, C. (2000). Comparative in vitro activity ofgatifloxacin, grepafloxacin, levofloxacin, moxifloxacin and trovafloxacin against4151 Gram-negative and Gram-positive organisms. Int J Antimicrob Agents, 14(1), 45–50.

. Mitchell, H. W., Cameron, J. L., Megan, S. J. A., Joseph, S., Alan, W., Anthony, C. G., & Ian, E. C. (2017). GC-MS analysis of Tasmannia lanceolata Extracts which Inhibit the Growth of the Pathogenic Bacterium Clostridium perfringens, Pharmacogn J., 9(5), 626-637.


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