Effect of PMF on Hepatitis Induced by Carbon Tetrachloride in Rats
Nowadays liver diseases constitute a major medical problem of worldwide proportions, so it is important to search for compounds that are more effective in their therapeutic action for hepatic diseases. The objective of this study is to evaluate the protective effects of PMF derived from camel urine on hepatitis induced in Wistar male rats by carbon tetrachloride (CCL4). Four groups of rats were treated as follows (Control, PMF orally, were intraperitoneally injected with CCL4 and CCL4 plus oral PMF) respectively. Physiological and histological alterations were evaluated after 7, 21, 42, 60 and 90 days. Highly significant decrease in the body weight and highly statistically increase of liver/body weight ratios were observed in rats treated with CCL4. Moreover, the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (GGT), alkaline phosphatase (ALP) and total bilirubin (TBL) were statistically increased. We also observed significantly decrease in total protein (TPRT) and total albumin (TALB). Additionally, the liver sections from rats injected with CCL4 showed liver fibrosis and necrosis, inflammatory cell aggregation and adipocyte deposition. Interestingly, the findings of this experimental study suggest that PMF may work as hepatoprotective agents against CCL4 induced hepatic damage by reducing the physiological and histological alterations. These results also suggest that the protective effect of PMF may be attributed to their antioxidant activities.
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