Antimicrobial Activity of Persea americana Peel Extract from North Sumatera, Indonesia , against Gram Positive and Gram Negative Bacteria in Vitro

Yuliani Mardiati Lubis, Zulkarnain Chaidir, Refilda Refilda, Abdi Dharma

Abstract


Lots of species of Indonesian plants are suspected to have potential as an antimicrobial, but not yet optimally utilized. Avocado peel (Persea americana) is one of the nutritional plant species can be used as an antimicrobial agent. The aim of the study was to determine the antimicrobial activity of ethanolic extract of Persea americana  peel (EEPA) against several bacteria. The extraction was conducted by maceration and concentrated using rotary evaporator using ethanol. The indicator bacteria used in this study were Staphylococcus aureus, Proteus vulgaris, Enteroccus faecalis, Escherichia coli and Pseudomonas aeruginosa. The concentration of EEPA  were 0,4%; 0,78%; 1,56%; 6,25%; 12,5%; 50%; 75% and 100%. The antimcriobial activity was conducted using disc diffusion method. The result showed that EEPA have antimicrobial activity against Gram positive and negative bacteria with the strongest inhibition zone at 100% and the weakest inhibition zone at 0,4%. 


Keywords


avocado peel; lime citrus peel; antimicrobial; Gram positive; Gram negative.

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References


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