Comparative Study on Genetic Variations in Maternal Antibody (IgY) Transfer from Dam to Egg-yolk in Different Meat Lines of Chickens
Keywords:
maternal antibody, immunoglobulin Y, egg-yolk, broiler sire lines, broiler dam lines.Abstract
The amount of maternal antibody transferred to the egg and the amount taken up by the developing chicks are important parameters that may greatly influence the health and survival of the chicks. Thus, the study of these parameters might provide a new likelihood in improving the livability of chicks. Considering the short life span of broiler chicks from hatch to slaughter, the importance of maternal antibodies is highly relevant. Therefore, the study was conducted to compare genetic variations in transfer of maternal immunoglobulin Y (IgY) through egg yolk in different broiler sire and dam lines namely: Male Line White (MLW), Male Line Colour (MLC), Male Line White 2 (MLW2), Female Line White (FLW), Female Line Colour (FLC) and a two-way cross of MLW male and FLW female (MLW x FLW). In total, 42 freshly laid eggs were collected from apparently healthy hens; 7 each from six different lines of chicken. Egg yolk IgY of the collected eggs was isolated by polyethylene glycol (PEG) precipitation method with slight modifications. Furthermore, the purity of the isolated IgY was examined by the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The egg yolk IgY levels were significantly (p<0.001) varied among the studied lines of chicken. The total amount of IgY per milliliter of yolk was the highest in FLW (30.904 ± 7.621) and the lowest in MLW (16.753 ± 5.282). However, the calculated total amount of yolk IgY (mg/egg) contained by the entire egg yolk was the highest (392.030 ± 136.185) in MLW2 and was the lowest (206.015 ± 61.058) in FLC and it was varied between 206.015 and 392.030 in the experimental lines of chicken. This study concluded that genotype of chickens has significant effect on transferability of maternal IgY through egg yolk in different meat lines of chickens.
References
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[2] N. M. Nemeth, P. T. Oesterle, R. A. Bowen. “Passive immunity to west nile virus provides limited protection in a common passerine species”. The American journal of tropical medicine and hygiene, 79; 283-290. 2008.
[3] R. Chalghoumi, Y. Beckers, D. Portetelle, A.Théwis. “Hen egg yolk antibodies (IgY), production and use for passive immunization against bacterial enteric infections in chicken: a review.” Biotechnology, Agronomy, Society and Environment, 13; 295-308. 2009.
[4] J. Kovacs-Nolan, Y. Mine. “Egg yolk antibodies for passive immunity.” Annual review of food science and technology, 3; 163-182. 2012.
[5] T. Diraviyam, B. Zhao, Y. Wang, R. Schade, A. Michael, X. Zhang. “Effect of chicken egg yolk antibodies (IgY) against diarrhea in domesticated animals: A systematic review and meta-analysis.” PloS one, 9; e97716. 2014.
[6] K. R. Hamal, S. C. Burgess, I. Y. Pevzner, G. F. Erf. “Maternal antibody transfer from dams to their egg yolks, egg whites, and chicks in meat lines of chickens.” Poultry Science, 85; 1364–1372. 2006.
[7] J. A. Cutting,T. F. Roth. “Changes in specific sequestration of protein during transport into the developing oocyte of the chicken.” Biochimica et Biophysica Acta, 298; 951-955. 1973.
[8] M. R. Loeken, T. R. Roth. “Analysis of maternal IgG subpopulations which are transported into the chicken oocyte.” Immunology, 49; 21-28. 1983.
[9] A. M. Ulmer-Franco. “Transfer of chicken immunoglobulin Y (IgY) from the hen to the chick.” Avian Biology Research, 5; 81-87. 2012.
[10] A. Lardinois, T. Van den Berg, B. Lambrecht, M. Steensels. “A model for the transfer of passive immunity against Newcastle disease and avian influenza in specific pathogen free chickens.” Avian Pathology, 43; 118-124. 2014.
[11] K. Kowalczyk, J. Daiss, J. Halpern, T. F. Roth. “Quantitation of maternal-fetal IgG transport in the chicken.” Immunology, 54; 755–762. 1985.
[12] A. S. Abdel-Moneim, M. Abdel-Gawad. “Genetic variations in maternal transfer and immune responsiveness to infectious bursal disease virus.” Veterinary Microbiology,114; 16-24. 2006.
[13] F. Davison, K. Magor, B. Kaspers. “Structure and evolution of avian immunoglobulins.” Avian Immunology. In: Davison F, Kaspers B, Schat KA, editors.. Elsevier: Academic press; 2008 .p.107-127.
[14] R. Schade, E. G. Calzado, R. Sarmiento, P. A. Chacana, J. Porankiewicz-Asplund, H. R. Terzolo. “Chicken egg yolk antibodies (IgY-technology): A review of progress in production and use in research and human and veterinary medicine.” ATLA, 33; 129-154. 2005.
[15] R. Agrawal, S. D. Hirpurkar, C. Sannat, A. K. Gupta. “Comparative study on immunoglobulin Y transfer from breeding hens to egg yolk and progeny chicks in different breeds of poultry.” Veterinary World, 9; 425-31. 2016.
[16] T. Diraviyam, T. Jeevitha, P. Saravanan, A. Michael, S. Meenatchisundaram.“Preparation of chicken (IgY) antibodies consortium for the prevention of enteric infections in poultry.” Journal of Microbiology and Biotechnology Research,1; 95-103. 2011.
[17] D. Carlander, M. Wilhelmson, A. Larsson. “Immunoglobulin Y levels in egg yolk from three chicken genotypes.” Food and Agricultural Immunology, 15 ; 35-40. 2003.
[18] R. Schade, C. Staak, C. Hendriksen, M. H. Erhard, H. Hugl, G. Koch, A. Larsson, W. Pollmann, M. Regenmortel, E. Rijke, H. Spielmann, H. Steinbusch, D. Straughan. “The production of avian (egg yolk) anitibodies: IgY.” ATLA. 24; 925-934. 1996.
[19] D. Pauly, P. A. Chacana, E. G. Calzado, B. Brembs, R. Schade. “IgY technology: ex-traction of chicken antibodies from egg yolk by polyethylene glycol (PEG) precipitation.” Journal of Visualized experiments, 51; 3084. 2011.
[20] A. Polson, M. B. von Wechmar, M. H. V. van Regenmortel. “Isolation of viral IgY antibodies from yolks of immunized hens.” Immunological Communications , 9; 475-493. 1980.
[21] U. K. Laemmeli. “Cleavage of structural proteins during the assembly of the head of bacteriophage T4.” Nature, 227; 680-685. 1970.
[22] R Core Team. R: “A language and environment for statistical computing.” Vienna: R Foundation for Statistical Computing. Available: http://www.R-project.org/. 2014.
[23] M. M. S. Dallal, E. Kalantar, L. K. Khorasani, M. H. H. Tehrani, M. Z. Zamani. “Identification and Extraction of Chicken Egg Yolk Immunoglobulin from Egg by Polyethylene Glycol (PEG) Precipitation.” Journal of Medical Bacteriology. 4;13-18. 2015.
[24] A. Polson. “Isolation of IgY from the yolk of eggs by a chloroform polyethylene glycol procedure.” Immunological Investigations, 19; 253-258. 1990.
[25] G. A. Leslie, L. W. Clem. “Phylogeny of immunoglobulin structure and function iii. Immunoglobulins of the chicken.” The Journal of Experimental Medicine, 130; 1337–1352. 1969.
[26] H. M. Chang, T. C. Lu, C. C. Chen, Y. Y. Tu, J. Y. Hwang. “Isolation of immunoglobulin from egg yolk by anionic polysaccharides.” Journal of Agriculture and Food Chemistry, 48; 995-999. 2000.
[27] H. Asemota, S. Curtello, A. A. J. Vaillant, W. Mohammed, S. Vuma, A. V. C. Rao, A. Kurhade, S. Kissoon, M. Smikle, B. Wisdom, G. Kurhadeet. “Purification of avian IgY with Trichloroacetic acid (TCA).” Journal of Chromatography & Separation Techniques, 4; 205. 2013.
[28] C. M. Almeida, C. L. Silva, H. P. Couto, R. C. Escocard, D. G. Rocha, L. P. Sentinelli, T. L. Kipnis, W. D. Silva. “Development of process to produce polyvalent IgY antibodies anti-african snake venom.” Toxicon, 52; 293-301. 2008.
[29] E. M. Akita, S. Nakai. “Immunoglobulins from egg yolk: isolation and purification.” Journal of Food Science, 57; 629-634. 1992.
[30] E. M. Akita, S. Nakai. “Production and purification of Fab' fragments from chicken egg yolk immunoglobulin Y (IgY).” Journal of Immunological Methods, 162; 155-164. 1993.
[31] C. Schwarzkopf, B. Thiele. “Effectivity of different methods for the extraction and purification of IgY.” Altex, 1; 35-39. 1996.
[32] D. Carlander, M. Wilhelmson, A. Larsson. “Limited day-to-day variation of IgY levels in eggs from individual laying hens.” Food and Agricultural Immunology , 13; 87–92. 2001.
[33] A. M. Ulmer-Franco, G. Cherian, N. Quezada, G. M. Fasenco, L. M. MacMullen.“Hatching egg and newly hatched chick yolk sac total IgY content at 3 broiler breeder flock ages.” Poltry Science, 91; 758-764. 2012.
[34] T. Deignan, J. Kelly, A. Alwan, H. J. Gert. “Comparative analysis of methods of purification of egg yolk immunoglobulin.” Food and Agricultural Immunology, 12 ; 77–85. 2000.
[35] X. Li, T. Nakano, H. H. Sunwoo, B. H. Paek, H. S. Chae, J. S. Sim. “Effects of egg and yolk weights on yolk antibody (IgY) production in laying chickens.” Poultry Science, 7; 266–270. 1998.
[36] U. Gadde, T. Rathinam, H. S. Lillehoj. “Passive immunization with hyperimmune egg-yolk IgY as prophylaxis and therapy for poultry diseases--A review.” Animal Health Research Reviews, 16; 163-176. 2015.
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2017-10-12
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Hussain, C. N. B., & Rahman, M. C. (2017). Comparative Study on Genetic Variations in Maternal Antibody (IgY) Transfer from Dam to Egg-yolk in Different Meat Lines of Chickens. American Scientific Research Journal for Engineering, Technology, and Sciences, 36(1), 357–369. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/3422
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