The Prevalence of Listeria monocytogenes In Placental Tissue from Abortion and Fetal Death at Mother and Child’s Sri Ratu Hospital, Medan, Indonesia

Mangatas Silaen, Ety Yerizel, Sumaryati Syukur, Endang Purwati

Abstract


For the identification of pathogenic bacteria Listeria monocytogenes (L.monocytogenes) in vitro, the method of examination that currently most reliable is the growth in the culture medium, followed by isolation and identification of biochemical and serological. Total of 98 samples were collected from placental tissue curettage resulted from abortion at Mother and Child's Sri Ratu Hospital Medan, North Sumatra, Indonesia. Curettage was undertaken in accordance with the working procedures issued by the research ethics committee of the Faculty of Medicine, University of Andalas Padang, West Sumatra, with a qualifying ethic examine, No: 190 / KEP / FK / 2015. The media we use to isolate and identification are Listeria Enrichment Broth (Biolife Italia) and Agar Listeria Ottaviani Agosti (ALOA) (Biolife Italia). All the tissues examined were found: Listeria (9%) include, L. monocytogenes isolates (90%), and L.innocua (10%). We conclude that L.monocytogenes has been found in placental tissue in less than 20  weeks of  abortion in Mother and Child’s Sri Ratu Hospital Medan, North Sumatra, Indonesia.

The aim of this study was to investigate other causes of abortion and fetal death in utero less than 20 weeks and whether L.monocytogenes has become another cause of abortion.


Keywords


Abortion; Placental tissue culture; L.monocytogenes.

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References


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