Spasmolytic Effects of Aqueous Extract of Sterculia setigera Delile on Isolated Rat Trachea

Judith F. Ahounou Aïkpe, André Hamadou, Géoffroy Ouedraogo, Sylvin Ouedraogo, Joachim D. Gbenou, Pierre H. Dansou


The spasmolytic properties of aqueous extract of Sterculia setigera was tested on Rat isolated trachea. Inhibition of the contraction was observed in presence of the aqueous extract (EC50 = 0.91mg/mL ± 0.02) after a pre contraction of the trachea by acetylcholine (10-5M). In the presence of propranolol (10-6M), the spasmolytic activity of the extract was inhibited. The EC50 value was 0.46mg/mL ± 0,11. The aqueous extract of Sterculia setigera also inhibited contraction induced by KCl (4.10-2 M) with EC50 value = 1.9mg/mL ± 0.48).  These results clearly show a relaxing effect of aqueous extract of Sterculia setigera on the trachea isolated from rat. This effect involves at least in part β-adrenergic receptor inhibition.


Sterculia setigera; bronchodilatory; spasmolytic; Rat trachea.

Full Text:



. C. Bakhoum, A. Samba, S. Ndiaye, B. Ndour. Sterculia setigera Del.: effet sur les cultures. Annals of forest science, ISSN 1286-4560, vol. 58, (2), pp. 207-215, 2001.

. E-J. Adjanohoun, A.M. Akyi, L. Aké Assi, J. Baniakina, J. Chibon, G. Cusset, V. Doulou, A. J. Enzanza, Eymé, E. Gondoté, A. Kéita, C. Mbemba, J. Mollet. Contribution aux études ethnobotaniques et floristiques en République Populaire du Congo, A.C.C.T. Ed., Paris, pp. 605, 1988.

. PJ. Houghton, R. Amala. Laboratory Handbook for the Fractionation of Natural Extracts. 1ère édition, CHAPMAN and HALL. 1998, pp 40-44. .

. M. Schramm Craig. β-adrenergic relaxation of rabbit tracheal smooth muscle: a receptor deficit improves with corticosteroid administration. Journal of pharmacology and experimental therapeutics, vol. 292, pp. 280-287, 2000.

. B. Loenders, M. Rampart and A.G. Herman. Selective M3 muscarinic receptor antagonists inhibit smooth muscle contraction in rabbit trachea without increasing the release of acetylcholine. Journal of pharmacology and experimental therapeutic, Vol. N°2, pp. 263-266, 1992.

. V.V. Van and A.R. Hulsmann. Autonomic innervation of human airways: structure, function, and pathophysiology in asthma. Neuroimmunomodulation, 6, pp.145-159, 1999.

. V. Kouloumenta, A. Hatziefthimiou, E. Paraskeva, K. Gourgoulianis and P.A. Molyvdas. Non-genomic effect of testosterone on airway smooth muscle. British Journal of Pharmacology, vol 149, pp. 1083–1091 2006.

. O. Arunlakshana and H. O. Schild. Some quantative uses of drugs antagonists. British Journal of Pharmacology, vol 14, pp.48-58, 1959.

. H. Kume and M.J. Kotilkoff. Muscarinic inhibition of single Kca channels in smooth muscle cell by pertussis-sensitive G protein. American Journal of Physiology, vol 261, pp. 1204-1209, 1991.

. A.F. Roffel, C.R. Elzinga, J. Zaagsma. Muscarinic M3 receptors mediate contraction of human central and peripheral airway smooth muscle. Pulm. Pharmacol., vol 3, pp. 47-51, 1990.

. T.J. Torphy and L.B. Cielslinkski. Characterization and selective inhibition of cyclic nucleotide phosphodiesterase isozymes in canine tracheal smooth muscle. Mol. Pharmacol., vol 37, pp. 206-214, 1990.

. M.T. Nelson, JB. Patlask, J.F. Worley, N.B. Standen. Calcium channels, potassium channels, and voltage dependance of arterial spooth muscle tone. American Journal of Physiology, 259 (Cell physiol), vol 28, pp. 3-18, 1990.

. O. Ezekiel, I. Walewaa, A. Anthony, B. Elujoba, A. Olanrewaju, A. Bankole. Invitro spasmolytic effect of aqueous extract of Calotropis procera on Guinea-pig trachea smooth muscle chain. Fitoterapia, vol 76, pp. 250–253, 2004.

. M. Ouedraogo, S. Ouedraogo, A.Traore, M. Lompo, N. Some, L. Sawadogo et I. P. Guissou. Propriétés bronchodillatatrices de l’extrait aqueux total de Tapinanthus dodoneifolus (DC. Danser) sur la trachée isolée de rat. Ethnopharmacologia. N° 35, pp. 44-49, 2005.


  • There are currently no refbacks.




About ASRJETS | Privacy PolicyTerms & Conditions | Contact Us | DisclaimerFAQs 

ASRJETS is published by (GSSRR).