A Review on Dried Blood Spots (DBS) as Alternative, Archival Material for Detection of Viral Agents (Measles, Mumps, Rubella, Hepatitis B Virus)
In recent years there appears a variety of new and innovative applications of the dried blood spots. The areas of their range of application are medicine, neonatology, virology, microbiology, toxicology and pharmacokinetics, metabolic exchange, therapeutic drug monitoring, toxicology, and control of environmental pollution. The advantages of DBS technology can be combined into four main groups: (1) compared to conventional venipuncture, requires less blood volume, which is especially important in pediatrics and neonatology; (2) the procedure for blood collection is easy, inexpensive and noninvasive; (3) the risk of bacterial contamination or hemolysis is minimal; and (4) DBS can be maintained for a long time with almost no impact on the quality of the analysis. In recent years is increasing the application of DBS as method for seroepidemiological survey with focus viral infections: measles, mumps, rubella and hepatitis B virus. The DBS technique is optimized as an alternative approach (non-invasive, inexpensive, not requiring trained staff and cold chain for transport and storage) of venipuncture collection of clinical material in virology.
This method facilitates the scientific researches about the concentration of virus specific antibodies in peripheral blood taken from a finger or heel; determining the percentage susceptibility / protection of the studied group of patients againt vaccine-preventable infectious - measles, mumps, rubella and hepatitis B; social benefits - non-invasive technique for testing of small children and infants and applications in regions in the countries with not well developed logistics infrastructure.
Schmidt, V., Bang, I. (1869 - 1918), founder of modern clinical microchemistry. (1986). Clin Chem. 32, (1), 213-215.
Hannon, W., Therell, B. (2014). Overview of the history and applications of dried blood spot samples. Dried blood spots. Applications and techniques. Li, W., Lee, M. S. 3, 3-15 Wiley Hoboken.
Bang, I., Bergmann, J., Der Blutzucker F. (1913). Wiesbaden Available from: https://archive.org/details/derblutzucker00bang.
Chapman, O. (1924). The complement-fixation test for syphilis. Use of patient's whole blood dried on filter paper. Arch Derm Syphilol., 9, (5), 607-611.
Smit, P., Elliott, I., Peeling, R., Mabey, D., Newton, P. (2014). An overview of the clinical use of filter paper in the diagnosis of tropical diseases. Am J Trop Med Hyg., 90, (2), 195-210.
Guthrie, R., Susi, A. (1963). A simple phenylalanine method for detecting phenylketonuria in large populations of newborn infants. Pediatrics, 32:338-343.
Alvarez-Munoz, M., Zaragoza-Rodriguez, S., Rojas-Montes, O., Palacios-Saucedo, G., Vazquez-Rosales, G., Gomez-Delgado, A., et al. (2005). High correlation of human immunodeficiency virus type-1 viral load measured in dried-blood spot samples and in plasma under different storage conditions. Arch Med Res, 36:382-386.
Dezateux, C. (1998). Evaluating newborn screening programmes based on dried blood spots: future challenges. Br Med Bull., 54(4):877-90.
Parker, S., Cubitt, W. (1999). The use of the dried blood spot sample in epidemiological studies. J Clin Patho., 52(9):633-639.
Ramakrishnan L., and Ruby G. (2009). Measurement of Glycated Hemoglobin A1c from Dried Blood by Turbidimetric Immunoassay. J Diabetes Sci Technol., 3(5): 1203–1206.
Beaudette, P., et al. (2004). Discovery Stage Pharmacokinetics Using Dried Blood Spots, J Chromatograph B., 809:153-158.
Barfield, M., et al. (2008). Application of Dried Blood Spots combined with HPLC-MS/MS for the quantification of acetaminophen in toxicokinetic studies, J Chromatograph B., 870:32-37.
Spooner, N., et al. (2009). Dried Blood Spots as a Sample Collection Technique for the Determination of Pharmacokinetics in Clinical Studies: Considerations for the Validation of a Quantitative Bioanalytical Method, Anal Chem., 81:1557-1563.
Demirev, P. (2013). Dried blood spots: analysis and applications. Anal Chem. 85, (2), 779-789.
Li, W., Lee, M. (2014). Dried blood spots. Applications and techniques. Li, W., Lee, M. S. VIII-IX Wiley Hoboken.
Rosalia, L., Maldonado-Rodriguez, A., Rojas-Montes O. et. al. (2009). Use of dried blood samples for monitoring hepatitis B virus Infection. Virology Journal, 6:153
Waterboer, T., Dondog, B., Michael, K. (2011). Dried Blood Spot Samples for Seroepidemiology of Infections with Human Papillomaviruses, Helicobacter pylori, Hepatitis C Virus, and JC Virus. Published OnlineFirst December 6, DOI: 10.1158/1055-9965.EPI-11-1001.
Blood Collection on Filter Paper for Newborn Screening Programs (2013); Approved Standard-Sixth Edition. Clinical and Laboratory Standards Institute (CLSI) Wayne, PA Available at: http://shop.clsi.org/c.1253739/site/Sample_pdf/NBS01A6_sample.pdf.
De Jesus VR, Zhang XK, Keutzer J, Bodamer OA, Mühl A, Orsini JJ, Caggana M, Vogt RF, Hannon WH (2009). Development and evaluation of quality control dried blood spot materials in newborn screening for lysosomal storage disorders. Clin Chem.; 55(1): 158–164.
McDade, T., Williams, S., Snodgrass, J. (2007). What a Drop Can Do: Dried Blood Spots as a Minimally Invasive Method for Integrating Biomarkers Into Population-Based Research. Demography, 44: 899–925.
Solomon, S.S., Solomon, S., Rodriguez, I., McGarvey, S., Ganesh, A., Thyagarajan, S., Mahajan, A., Mayer, K. (2002). Dried blood spots (DBS): a valuable tool for HIV surveillance in developing/tropical countries. Int J STD AIDS; 13:25-8.
World Health Organization. Eliminating measles and rubella and preventing congenital rubella infection, WHO European region strategic plan 2005–2010.
Marinova, L., Kojouharova, M., Mihneva, Z. (2009). An ongoing measles outbreak in Bulgaria, 2009. Euro Surveill., 14(26)s
Manual for the laboratory diagnosis of measles and rubella virus infection - Second edition World Health Organization 2007.
Calabrese, L., Zein, N., Vassilopoulos, D. (2006). Hepatitis B virus (HBV) reactivation with immunosuppressive therapy in rheumatic diseases: assessment and preventive strategies. Ann Rheum Dis, 65: 983-989
Yeo, W., Johnson, P. (2006). Diagnosis, prevention and management of hepatitis B virus reactivation during anticancer therapy. Hepatology, 43:209-220
Gigi, E., Georgiou, T., Mougiou, D., Boura, P., Raptopoulou-Gigi, M. (2013). Hepatitis B reactivation in a patient with rheumatoid arthritis with antibodies to hepatitis B surface antigen treated with rituximab. Hippokratia, 17 (1): 91-93
Basavaraju, S., Pitman, J. (2014) Dried blood spots for use in HIV-related epidemiological studies in resource-limited settings. Dried blood spots. Applications and techniques. Li, W., Lee, M. S. 76-94 Wiley Hoboken.
Gakhar, H., Holodniy, M. (2014). Use of dried blood spot samples in HCV-, HBV-, and influenza-related epidemiological studies. Dried blood spots. Applications and techniques. Li, W., Lee, M. S. 3, 95-113 Wiley Hoboken.
Marques, B., et al. (2012). Dried blood spot samples: optimization of commercial EIAs for hepatitis C antibody detection and stability under different storage conditions. J Med Virol. 84, (10), 1600-1607.
Larrat, S., et al. (2012). Performance of an antigen–antibody combined assay for hepatitis C virus testing without venipuncture. J Clin Virol. 55, (3), 220-225.
Uzicanin, A., Lubega, I., Nanuynja, M., Mercader, S., Rota, P., Bellini, W., Helfand R. (2011). Dried Blood Spots on Filter Paper as an Alternative Specimen for Measles Diagnostics: Detection of Measles Immunoglobulin M Antibody by a Commercial Enzyme Immunoassay. The J of Infec Dis, 204:S564–S569.
Helfand, R., Cabezas, C., Abernathy, E., Castillo-Solorzano, C. et. al. (2007). Dried Blood Spots versus Sera for Detection of Rubella Virus-Specific Immunoglobulin M (IgM) and IgG in Samples Collected during a Rubella Outbreak in Peru. Clinical and Vaccine Immunology, Vol. 14, No. 11, p. 1522–1525.
Grüner, N., Stambouli, O., Ross, R. (2015). Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques. J. Vis. Exp. (97), e52619, doi:10.3791/52619.
WHO manual for HIV drug resistance testing using dried blood spot specimens (2012). World Health Organisation (WHO).
Mei, J., Lee, M. (2014). Dried blood spots sample collection, storage, and transportation. Dried blood spots. Applications and techniques. Li, W., Lee, M. S. 21-31 Wiley Hoboken.
Zimmermann, R. (2012). DRUCK-Studie – Drogen und chronische Infektionskrankheiten in Deutschland. Ergebnisse der Pilotierung eines Sero- und Verhaltenssurveys bei i. v. Drogengebrauchern. Epidemiol Bull. 33, 335-339.
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