Seroepidemiological Analysis of Contagious Caprine Pleuropneumonia through cELISA in Selected Districts of Khyber Pakhtunkhwa-Pakistan

Inamullah Wazir, Iftikhar Hussain, Mirza Ali Khan, Muhammad Ijaz Ali, Hanif Ur Rahman, Faiza Ashraf, Shahid Khan, Baitullah Khan, Sibghat Ullah, Qudrat Ullah, khyber khyber, Asaf Khan


Contagious caprine pleuropneumonia (CCPP) is a fatal disease of goats caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp).This disease has been causing huge economic losses to goat rearing farmers in Khyber Pakhtunkhwa.  Seroepidemiological study of this disease was conducted for the first time in selected districts of Khyber Pakhtunkhwa namely Swat, Peshawar, Kohat and Dera Ismail Khan. Total 384 serum samples were collected randomly from goats having different ages and both sexes showing respiratory signs belonging to flocks with no vaccination history against CCPP. The serum samples were examined for Mccp directed antibodies using monoclonal antibody based cELISA. Out of total 384 samples 15 samples were detected positive on cELISA with 3.91% overall seroprevalence in the selected districts. The highest CCPP seroprevalence was recorded in district Swat (8.33%) followed by district Kohat and D.I Khan (3.13% in each district) and the lowest seroprevalence was observed in district Peshawar (1.04%). Age based CCPP seroprevalence was found highest (6.73%) in the goat kids of age 1 to 180 days followed by the (3.85%) young goat of 181 to 365 days while the lowest was found in the adult goats (2%) with age more than one year.

Sex based seroprevalence was found more in female goats (4.01%) as compare to male goats (3.33%). This study determined the load of CCPP using highly specific monoclonal antibody based cELISA kit in the selected districts of Khyber Pakhtunkhwa for the first time. Further study on CCPP is needed with increased sample size, that cover wider region of Khyber Pakhtunkhwa as necessary for limiting this disease through effective control measures. 


Mycoplasma; Mccp; cELISA; CCPP.

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A. Gelagay, S. Teshale, W. Amsaluc and G. Esayas. (2007). “Prevalence of contagious caprine pleuropneumonia in the Borana pastoral areas of Ethiopia”. Small Ruminant Research, 70: 131-135, 2007.

A. Peyraud, F. Poumarat, F. Tardy, L.M. Silván, K. Hamroev, T. Tilloev, M. Amirbekov, K. Tounkara, C. Bodjo, H. Wesonga, I.G. Nkando, S. Jenberie, M. Yami, E. Cardinale, D. Meenowa, M.R. Jaumally, T. Yaqub, M.Z. Shabbir, N. Mukhtar, M. Halimi, G.M. Ziay, W. Schauwers, H. Noori, A.M, Rajabi, S. Ostrowski and F. Thiaucourt. “An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based Celisa”. BMC Veterinary Research, 10, 48-57, 2014.

A.B. Zahur, M. Ashfaque, S.U. Rehman, M. Afaq and M. Akhtar. (1994). “Comparison of single radial haemolysis and indirect haemagglutination tests for assaying antibodies against Mycoplasma mycoides subspecies capri”. Pakistan Veterinary Journal, 14, 119-122, 1994.

E.R. Nascimento, M.G.F. Nascimento, E.A. Freundi and H. Andrsen. “Isolation of Mycoplasma mycoides from outbreaks of caprine mycoplasmosis in Brazil”. British Veterinary journal, 142(3), 246-257, 1986.

H. Ejaz, H. Hashmi, M.A. Awan, M.A. Kakar, M.N. Shahwani, S. Ameen, F.A. Bukhari, T. Hameed and M.M. Tariq. “Molecular Study on the Prevalence of Respiratory Mycoplasma Species in Small Ruminants of Kuchlak, District Quetta and Khanozai, District Pishin, Balochistan”. Pakistan Journal of Zoology, 47(2), 473-478, 2015.

M.A. Awan, F. Abbas, M. Yasinzai, R.A.J. Nicholas, S. Babar, R.D. Ayling, M.A. Attique and Z. Ahmed. “Prevalence of Mycoplasma capricolum subsp. capricolum and Mycoplasma putrefaciens in goats in Pishin District of Balochistan”. Pakistan Veterinary Journal, 29(4), 179-185, 2009.

R.A.J Nicholas. “Improvements in the diagnosis and control of diseases of small ruminants caused by mycoplasmas”. Small Ruminant Research, 45, 145-149, 2002.

S. Woubit, S. Lorenzon, A. Peyraud, L.M. Silvan and D.I.M. Silvan. “A specific PCR for the identification of Mycoplasma capricolum subsp. capripneumoniae, the causative agent of contagious caprine pleuropneumonia (CCPP)”. Veterinary Microbiology, 104, 125-132, 2004.

S.A. Atim, C. Ayebazibwe, F.N. Mwiine, J. Erume and R. Tweyongyere. “A Survey for Contagious Caprine Pleuropneumonia in Agago and Otuke Districts in Northern Uganda”. Open Journal of Veterinary Medicine, 6, 9-14, 2016.

T.O. Abegunde, H.E. Adler, T.B. Farver and A.J. DaMassa. “A serologic survey of Mycoplasma putrefaciens infection in goats”. American Journal of Veterinary Research, 42(10), 1798-801, 1981.

Verbisck-Bucker, G.M. Gonzalez-Candela, J. Galian, M.J. Cubero-Pablo, P. Martin-Atance and L. Leon-Vizcaino. “Epidemiology of Mycoplasma agalactiae infection in free-ranging Spanish Ibex (Carpra pyrenaica) in Andalusia, Southern Spain”. Journal of Wild life Diseases, 44(2), 369-380, 2008.

W. Shahzad, R. Munir, M.S. Khan, M.U.D. Ahmad, M.A. Khan, M. Ijaz, M. Shakil, M. Iqbal and R. Ahamd. “Characterization, Molecular Diagnosis and Prevalence of Caprine Mycoplasmosis in Different Areas of Pakistan”. Pakistan Journal of Zoology, 44(2), 559-568, 2012.

W. Shahzad, T. Yaqoob, N. Mukhtar, R. Munir, R. Ahmad, M.S. Khan and A. Hussain. “sero-prevalence of mycoplasma capricolum subsp.capripneumoniae in goats through cELISA in different districts of punjab, Pakistan”. The Journal of Animal & Plant Sciences, 26(4), 931-937, 2016.


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