Bcl2, Bax and LMP1 Genes Expression in Uterine Leiomyoma Tissue According to Vitamin D Status among Congolese Women

Authors

  • P Ingala Department of Obstetrics and Gynecology, University of Kinshasa Hospital,Kinshasa,00243,Démocratic Republic of Congo
  • B Lebwaze Department of Pathology, University Of Kinshasa,Kinshasa,00243,Démocratic Republic of Congo
  • J Mboloko Department of Obstetrics and Gynecology, University of Kinshasa Hospital,Kinshasa,00243,Démocratic Republic of Congo
  • F Lepira Department of Internal Medicine, University of Kinshasa ,Kinshasa,00243,Démocratic Republic of Congo
  • P Kayembe Public Health School of Kinshasa ,Kinshasa,00243,Democratic Republic of Congo
  • N Pakasa Department of Pathology, University Of Kinshasa,Kinshasa,00243,Démocratic Republic of Congo
  • A Tshiband Division of radio immunologie,Regional Center of Nuclear study of Kinshasa,Kinshasa,00243,Democratic Republic of Congo
  • A Mputu Department of Obstetrics and Gynecology, University of Kinshasa Hospital,Kinshasa,00243,Démocratic Republic of Congo

Keywords:

Bcl 2, Bax, and LMP1 genes expression, Uterine leiomyoma tissue, vitamin D status.

Abstract

The objective of this paper is to describe genes expression patterns of the anti-apoptotic Bcl2 and pro apoptotic Bax as well as EBV infection marker LMP1 in, leiomyoma tissue according to patients vitamin D status. To investigate the relationship between alterations of genes expression patterns and serum vitamin D levels, samples from 105 women undergoing surgery for uterine leiomyoma in 6 hospitals in Kinshasa from April 1 to October 31, 2014 were obtained for genes expression analysis of Bcl-2, Bax and LMP-1 genes. Genes expression in leiomyomas was measured by immunohistochemistry. Serum vitamin D levels were determined by IRMA. Association between vitamin D status and genes expression was assessed using logistic regression analysis. From 105 women providers of leiomyoma tissues examined, 41 were sufficients in vitamin D, 36 were insufficients and 28 deficients. In uterine leiomyoma tissues obtained Bcl2 was expressed in 96 (91.4%) of samples with low, moderate and high expression observed in 33.3%, 32.4% and 25.7%, respectively. The differences in Bcl2 expression between the three subgroups of vitamin D categories were not statistically significant.

As a mirror of Bcl2 expression, Bax protein was not expressed in the majority (n = 95, 90.5%) of samples. Similar to Bcl2, the differences in Bax expression between the three subgroups of vitamin D categories did not reach the level of statistical significance. Of the 105 leiomyoma tissue examined, LMP1 was observed in 30 (28.6%) of samples and showed a tendency towards increased expression with the decline in vitamin D levels; however, the tendency was fairly not statistically significant.

In multivariate analysis, predictors of Bcl2 expression were age, parity, and overweight/obesity and insufficiency/deficiency vitamin D; however, the differences observed were not statistically significant. The same predictors were also associated with Bax expression but once again the observed differences were not statistically significant. Vitamin D deficiency, the only predictor significantly associated with LMP1 expression, conferred a 3.9 fold greater risk (aOR 3.9; 95% CI 1.068-14.242; p = 0.039) of expressing LMP1 in leiomyoma tissue.

In the present study, Bcl2 gene expression in ULM tissues tended to increase with the decline in vitamin D levels but observed differences were not statistically significant. In contrast, LMP1 gene expression was significantly associated with vitamin D deficiency. In spite of methodological limits, these findings do suggest a role of Vitamin D deficiency in the development and progression of ULM.

References

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Published

2016-07-20

How to Cite

Ingala, P., Lebwaze, B., Mboloko, J., Lepira, F., Kayembe, P., Pakasa, N., Tshiband, A., & Mputu, A. (2016). Bcl2, Bax and LMP1 Genes Expression in Uterine Leiomyoma Tissue According to Vitamin D Status among Congolese Women. American Scientific Research Journal for Engineering, Technology, and Sciences, 22(1), 50–62. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/1826

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Vol. 22 No. 1 (2016)

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