Microbiology and Antibiotic Sensitivity Pattern of Diabetic Foot Ulcers Patients

Authors

  • Ary Putra Noor Resident of Department of Orthopaedic and Traumatology, Faculty of Medicine and Nursing, RSUP dr. Sardjito, Jalan Kesehatan no. 1, Yogyakarta, Indonesia, 55284
  • Puntodewo Puntodewo Staff of Department of Orthopaedic and Traumatology, Faculty of Medicine and Nursing, RSUP dr. Sardjito, Jalan Kesehatan no. 1, Yogyakarta, Indonesia, 55284

Keywords:

Diabetic foot ulcer, Bacterial pattern, Antibiotic sensitivity, Acinetoobacter baumanii.

Abstract

One of complications in diabetes mellitus which occurs in the foot, due to a combination of peripheral vasculopathy, neuropathy and abnormal foot pressure, followed by immunopathy and infections is diabetic foot ulcer [1,2]. Those are a major medical, social, and economic problem and are the leading cause of hospitalization for patients with diabetes. Severe infections can lead to amputations and increasing the burden both to the patient and the hospital2. This study aims to know the microbiology pattern and the antibiotic sensitivity pattern for diabetic diabetic foot ulcer in Sardjito General Hospital. The data were obtained from Clinical Pathology Laboratory Sardjito Hospital. Subject in this study were diabetic foot ulcer patients whom hospitalized and undergo surgical procedure between January 2013, until January 2015. Foot ulcers samples acquired from wound bed during surgery were analyzed microbiologically to provide information about the bacterial pattern and their antibiotic sensitivity. From 64 patient samples analyzed, 27 different kind of bacteria were obtained. Acinetobacter baumanii was the most frequent bacteria isolated from patient in this study (25%), followed by Enterococcus faecalis (8%) and pseudomonas aeruginosa (8%).  The antibiotic sensitivity test was performed using 31 antibiotics, and the result was tabulated. Infection with multi drug resistant bacteria is common in diabetic foot ulcers and is associated with increased requirement for surgical treatment. This study proved that multidrug resistant bacteria also a complicating factor in Sardjito Hospital. There is a need for continuous surveillance of bacteria involved in diabetic foot infections to help determine the choice of suitable antibiotic selection.

References

. S. Singh, D. R. Pai, and C. Yuhhui , “Diabetic Foot Ulcer – Diagnosis and Management,” Clinical Research on Foot & Ankle, vol. 01, no. 03, 2013.

. Mathangi, T, Prabhakaran, P. “Prevalence of Bacteria Isolated from Type 2 Diabetic Foot Ulcers and the Antibiotic Susceptibility Pattern,” International Journal of Current Microbiology and Applied Science, Vol. 2 No. 10, pp. 329-337, 2013.

. American Diabetes Association, “Diagnosis and Classification of Diabetes Mellitus,” Diabetes Care, vol. 35, no. Supplement 1, 2011.

. M. Harris, "Diabetes in America, 2nd Edition", The National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, 1995.

. S. Pendsey, "Understanding diabetic foot", International Journal of Diabetes in Developing Countries, vol. 30, no. 2, p. 75, 2010. Available: 10.4103/0973-3930.62596

. K. Ziegler-Graham, E. MacKenzie, P. Ephraim, T. Travison and R. Brookmeyer, "Estimating the Prevalence of Limb Loss in the United States: 2005 to 2050", Archives of Physical Medicine and Rehabilitation, vol. 89, no. 3, pp. 422-429, 2008. Available: 10.1016/j.apmr.2007.11.005

. G. Leese et al., "Use of antibiotics in people with diabetic foot disease: A consensus statement", The Diabetic Foot Journal, vol. 12, no. 2, pp. 1-10, 2009.

. P.A Yildiz, T. Ozdil, M. Dizbay, Ö.G Tunccan and K. Hizel, "Peripheral arterial disease increases the risk of multidrug-resistant bacteria and amputation in diabetic foot infections", TURKISH JOURNAL OF MEDICAL SCIENCES, vol. 48, no. 4, pp. 845-850, 2018. Available: 10.3906/sag-1803-217.

. E. J. C. Goldstein, D. M. Citron, and C. A. Nesbit, “Diabetic Foot Infections: Bacteriology and activity of 10 oral antimicrobial agents against bacteria isolated from consecutive cases,” Diabetes Care, vol. 19, no. 6, pp. 638–641, 1996.

. S. Sekhar, N. Vyas, M. Unnikrishnan, G. Rodrigues and C. Mukhopadhyay, "Antimicrobial susceptibility pattern in diabetic foot ulcer: A pilot study", Annals of Medical and Health Sciences Research, vol. 4, no. 5, p. 742, 2014. Available: 10.4103/2141-9248.141541.

. B. Lipsky et al., "2012 Infectious Diseases Society of America Clinical Practice Guideline for the Diagnosis and Treatment of Diabetic Foot Infections", Clinical Infectious Diseases, vol. 54, no. 12, pp. e132-e173, 2012. Available: 10.1093/cid/cis346.

. S. Singh, S. Tiwari, D. Pratyush, A. Dwivedi, S. Gupta and M. Rai, "Microbiological and clinical characteristics of diabetic foot infections in northern India", The Journal of Infection in Developing Countries, vol. 6, no. 04, 2012. Available: 10.3855/jidc.1827.

. A. J. K. Ibrahim, S. M. Bhatawadekar, Arunima, B. Y. Peerzada, M. M. Modak, and K. K. Lahiri, “Bacterial Profile of Diabetic Foot Ulcer - Study From Western India,” International Journal of Health Sciences and Research, vol. 6, no. 5, pp. 65–71, May 2016.

. N. Cardoso, L. Cisneiros, C. Machado, J. Cenedezi, R. Procopio and T. Navarro, "Bacterial genus is a risk factor for major amputation in patients with diabetic foot", Revista do Colégio Brasileiro de Cirurgiões, vol. 44, no. 2, pp. 147-153, 2017. Available: 10.1590/0100-69912017002007.

. A. Howard, M. O’Donoghue, A. Feeney and R. Sleator, "Acinetobacter baumannii", Virulence, vol. 3, no. 3, pp. 243-250, 2012. Available: 10.4161/viru.19700.

. S. A. Mazumder, “Acinetobacter Medication: Antibiotics,” Acinetobacter Medication: Antibiotics, 02-Feb-2019. [Online]. Available: https://emedicine.medscape.com/article/236891-medication. [Accessed: 27-Mar-2019].

. M. E. Pachon-Ibanez, M. E. Jimenez-Mejias, C. Pichardo, A. C. Llanos, and J. Pachon, “Activity of Tigecycline (GAR-936) against Acinetobacter baumannii Strains, Including Those Resistant to Imipenem,” Antimicrobial Agents and Chemotherapy, vol. 48, no. 11, pp. 4479–4481, 2004.

. W. Ni, Y. Han, J. Zhao, C. Wei, J. Cui, R. Wang, and Y. Liu, “Tigecycline treatment experience against multidrug-resistant Acinetobacter baumannii infections: a systematic review and meta-analysis,” International Journal of Antimicrobial Agents, vol. 47, no. 2, pp. 107–116, 2016.

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Published

2019-03-29

How to Cite

Putra Noor, A., & Puntodewo, P. (2019). Microbiology and Antibiotic Sensitivity Pattern of Diabetic Foot Ulcers Patients. American Scientific Research Journal for Engineering, Technology, and Sciences, 54(1), 49–55. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/4755

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