Antioxidant and Anti-elastase Activity of Seed and Peel Extract of P.edulis

Krusty Vera; Ahmad Raif; Refi Ikhtiari

Krusty Vera Master Program of Biomedical Sciences, Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia
Ahmad Raif Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia
Refi Ikhtiari Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia

Keywords: Passiflora edulis, antioxidant, elastase inhibitor, anti-aging.

Abstract

Passiflora edulis is a woody climber, native in Brazil and now cultivated in all parts of the world, chiefly for its edible fruits and for ornamental flowers. The seed and the peel of P.edulis always removed and do not have economical values. The aim of the study was to determine the antioxidant activity of the seed and peel of P.edulis and its potential as an elastase inhibitor. The seed and the peel were extracted using ethanol 70%. The extracts were evaporated and the antioxidant activity was determined using H2O2 scavenging method. The IC50 values of H2O2 scavenging activity of peel and seed extract were 626.31 ± 2.10 µg/mL and 2106.46 ±33.21 µg/mL respectively. the effect of variation concentration of seed and peel extract showed that the antioxidant activity was dose-dependent manner. The IC50 value of elastase inhibitor of peel and seed extract was 62.82 ± 1.50 µg/mL and 41.06 ± 0.31 µg/mL respectively. This result gave the promising effect of peel and seed extract of P.edulis as an anti-aging cosmetic ingredient.

References

L. Santos et al., “Extraction Parameters Affect Flavonoids Content and Antioxidant Activities in Passiflora edulis,” J. Chem. Pharm. Res., vol. 8, no. 10, pp. 99–107, 2016.

M. Sunitha and K. Devaki, “Antioxidant Activity of Passiflora edulis Sims Leaves Ion Pair-HPLC Method for the Simultaneous Estimation of Quinapril and Hydrochlorothiazide in Tablets,” Indian J. Pharm. Sci., no. June, pp. 310–311, 2009.

L. M. Uwa, “The Anti-aging Efficacy of Antioxidants,” Curr Trends Biomed. Eng Biosci, vol. 7, no. 4, pp. 2–4, 2017.

I. Binic, V. Lazarevic, M. Ljubenovic, J. Mojsa, and D. Sokolovic, “Skin Ageing : Natural Weapons and Strategies,” Evidence-Based Complement. Altern. Med., vol. 2013, pp. 1–10, 2013.

J. H. Kim, J. C. Byun, A. Kumar, R. Bandi, C. Hyun, and N. Ho, “Compounds with elastase inhibition and free radical scavenging activities from Callistemon lanceolatus,” J. Med. Plant Res., vol. 3, no. 11, pp. 914–920, 2009.

W. Widowati et al., “Antioxidant and Anti Aging Assays of <I>Oryza sativa</I> Extracts, Vanillin and Coumaric Acid,” J. Nat. Remedies, vol. 16, no. 3, p. 88, 2016.

T. Nithiya and R. Udayakumar, “In Vitro Antioxidant Properties of Phloretin — An Important Phytocompound,” J. Biosci. Med., vol. 4, no. January, pp. 85–94, 2016.

Z. Huyut, F. Beydemir, and E. Gülçin, “Antioxidant and Antiradical Properties of Selected Flavonoids and Phenolic Compounds,” Biochem. Res. Int., vol. 2017, pp. 1–10, 2017.

C. Irawan, L. Sulistiawaty, and H. Rochaeni, “Evaluation of DPPH free radical scavenging activity of Pometia pinnata from Indonesia,” Pharma Innov. J., vol. 6, no. 8, pp. 403–406, 2017.

Y.S.Wong. C.M.Sia. H.E.Khoo. Y.K.Ang. S.K.Chang. H.S.Yim, “PROPERTIES OF PASSION FRUIT ( PASSIFLORA EDULIS ) PEEL,” Acta Sci. Pol.,Technol. Aliment, vol. 13, no. 3, pp. 257–265, 2014.

I. Fidrianny, A. Rizkiya, and K. Ruslan, “Antioxidant activities of various fruit extracts from three solanum sp. using DPPH and ABTS method and correlation with phenolic, flavonoid and carotenoid content,” J. Chem. Pharm. Res., vol. 7, no. 5, pp. 666–672, 2015.

H. Kadum et al., “Applications of Date ( Phoenix dactylifera L .) Fruits as Bioactive Ingredients in Functional Foods,” vol. 12, no. 3, pp. 1101–1108, 2018.

A. Sahasrabudhe and M. Deodhar, “Anti-hyaluronidase, anti-elastase activity of Garcinia indica,” International Journal of Botany, vol. 6, no. 3. pp. 299–303, 2010.