Effect of White Turmeric Rhizome Extract (Curcuma zedoaria) on Testis Histology of Male Wistar Rat
Traditional medicine is an alternative in medicine because of the minimal side effects and low cost. One of the spices that are widely grown in Indonesia and used for traditional medicine is white turmeric rhizome. White turmeric rhizome has a chemical content such as curcumin which can be used as an anticancer. The function of white turmeric rhizome as an anticancer is not selective and may interfere with the normal cell function that actively performs mutation such as seminiferous tubule cells can cause antifertility effect. To find out the antifertility effects of white turmeric rhizomes in the testis, a rat was examined by looking at the histology of testis due to the effects of white turmeric rhizome. The study was conducted for two months from April 2019 until June 2019. All experimental procedures were performed in accordance with the Medical Research Ethics Committee Faculty of Medicine University of Prima Indonesia. Research of the animals were carried out in experimental cages at the Pharmacology and Toxicology Laboratory, Faculty of Pharmacy, University of North Sumatera Medan, while testis histopathology was made in Department of Histology, Faculty of Medicine, University of North Sumatera Medan. This research used 24 white male Wistar rats (Rattus noverticus), with ages 6-8 weeks and weighing 160-200 grams. Samples were grouped randomly into 4 groups, each group consisting of 6 rats. Each group then was given ethanol extract of white turmeric rhizome obtained from UPT Materia Medica Batu, East Java, which was determined based on the method of calculating conversion factors with doses of ethanol extract of white turmeric rhizome 20, 40 and 60 mg / 200 gram rats and control groups. All data obtained then tested using the normality test and Shapiro-Wilk and the result is normal distributed data (P> 0.05), then continued with the variance analysis test (ANOVA) obtained p value <0.05. The results showed that there was a significant difference between the decrease in the number of spermatogenic cell layers and mitosis count due to the effects of white turmeric rhizome.
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