Antioxidant and Antifungal Activities of Cocoa Butter (Theobroma cacao), Essential Oil of Syzygium aromaticum and a Combination of Both Extracts against Three Dermatophytes

Patience Mekemzeu Fankem, Sylvie Nguikwie Kwanga, Modeste Lambert Sameza, François Tchoumbougnang, Raymond Tchabong, Leopold Tatsadjieu Ngouné, Pierre Michel Jazet Dongmo

Abstract


To contribute in the research of better drugs against dermatophytosis, we evaluated the antioxidant and antidermatophytic activities of cocoa butter, cloves essential oil, and a mixture of both extracts. The cocoa butter was obtained by boiling the cocoa paste. The essential oil extracted by hydrodistillation was chemically analysed by gas chromatography and gas chromatography coupled with mass spectrometry. The antioxidant activity was determined using the DPPH scavenging method, and  the antidermatophytic activity was evaluated using the agar dilution method. The essential oil, majoritary constituated by eugenol (87.62%), β-caryophyllene (5.88%), and β-bisabolene (4.41%), had an antiradical power (4.22 x 10-2) higher than that of BHT (4.00 x 10-3), like the cocoa butter and essential oil mixture (6.06 x 10-3). The essential oil was more active than the griseofulvin: it was fungicidal at 400 ppm against Trichophyton rubrum, and at 900 ppm against Microsporum gypseumand Trichophyton tonsurans.

The cocoa butter activity was low, but the mixture with the essential oil had an important activity with inhibitory percentages of 78.69 %, 88.27 %, 91.20% against T. rubrum (at 400 ppm), T. tonsurans(at 900 ppm)and M. gypseum (at 900 ppm)respectively. Cloves essential oil and the mixture with cocoa butter can be used to formulate new drugs against dermatophytes. 


Keywords


Antioxidant; antidermatophytic; cocoa butter; cloves; essential oil.

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References


A.J.G. Araújo, M.A.J. Souza, O.M. Bastos, J.C. Oliveira. "Occurrence of onychomycosis among patients attended in dermatology offices in the city of Rio de Janeiro, Brazil". Anais Brasileiros De Dermatologia journal, vol 78, pp. 299-308, 2003.

A. Sepahvand, J. Abdi, Y. Shirkhani, Sh. Fallahi, M. Tarrahi, S. Soleimannejad. "Dermatophytosis in Western Part of Iran, Khorramabad". Asian Journal of Biological Sciences, vol 2, pp. 58-65, 2009.

E.R. Siqueira, J.C. Ferreira, C.M.L. Maffei, R.C. Candido. "Occurrence of dermatophyte, in nails, feet and hands of universitary students". Revista da Sociedade Brasileira de Medicina Tropical, vol 39, pp.269-71, 2006.

V. Arya, S. Yadav, S. Kumar, J.P. Yadav. "Antimicrobialactivity of Cassia occidentalis L. (leaf) againstvarioushumanpathogenic microbes". Life science and medicalresearch journal, vol 9, pp. 1-11, 2010.

P.N. Chowdhry, S.L. Gupta, N. Anand. "Diversity of Fungi as Human Pathogen".Recent Research in Science and Technology, vol 5, pp. 17-20, 2013.

T. Jiofack, C. Fokunang, N.M. Guedje, V. Kemeuze, E. Fongnzossie, B.A. Nkongmeneck, et al. "Ethnobotanical uses of medicinals plants of two ethnoecological regions of Cameroon". International Journal of Medicine and Medical Sciences, vol 2, pp. 60-79, 2010.

K.R.Park, D. Nam, H.M. Yun, S.G. Lee, H.J. Jang, G. Sethi, et al. "Beta-caryophyllene oxide inhibitsgrowth and induces apoptosis through the suppression of PI3K/AKT/mTOR/S6K1 pathways and ROS-mediated MAPKs activation". Journal of cancer letters, vol 312, pp. 178-88, 2011.

E. Pinto, L. Vale-Silva, C. Cavaleiro, L. Salgueiro. "Antifungalactivity of the clove essential oil from Syzygium aromaticumon Candida, Aspergillus and dermatophyte species". Journal of Medicine and Microbiology, vol 58, pp. 1454-462, 2009.

D. Webster, P. Taschereau, R. J. Belland, C. Sand, R. P. Rennie. "Antifungal activity of medicinal plant extracts; preliminary screening studies". Journal of Ethnopharmacology, vol 115, pp. 140-46, 2008.

J.C.N. Assob, H.L.F. Kamga, D.S. Nsagha, A.L. Njunda, P.F. Nde, E.A. Asongalem, et al. "Antimicrobial and toxicological activities of five medicinal plant species from Cameroon Traditional Medicine in BMC". Complementary and Alternative Medicine, vol 11, pp. 70, 2011.

Y. Kongo-Nzuzi. "Evaluation in vitro des pouvoirs antifongiques des extraits des feuilles de papayer sur des souches de Candida albicans".Graduéeen techniques de laboratoire, Institut Supérieur des Techniques Médicales, Kinshasa, 2009.

A.D. Afagnigni. "Evaluation des activités antibactériennes et antifongiques de Dracaena deisteliana(Dracaenaceae) ". Mémoire présenté en vue de l’obtention du Diplôme de Professeur de l’Enseignement Secondaire, 2ème Grade, Université de Yaoundé I, Cameroun, 2013.

S. Murugan, D. P. Uma, P. N. Kannika, K.R. MANI. "Antimicrobial activity of SyzygiumJambosagainst selected human pathogens".International Journal of Pharmacy and Pharmaceutical Sciences, vol 3, pp.44-47, 2011.

M.A. Martín, S. Ramos, I. Cordero-Herrero, L. Bravo, L. Goya."Extrait de cacao phénolique protège les cellules bêta du pancréas contre le stress oxydatif". Journal of Nutriments, vol 5, pp. 2955-968, 2013.

G.A. Alitonou, F.P. Tchobo, F. Avlessi, B. Yehouenou, P. Yedomonhan, A.Y. Koudoro, et al. "Chemical and biological investigations of SyzygiumaromaticumL. essential oil from Benin".International Journal of Biological and Chemical Sciences, vol 6, pp. 1360-367, 2012.

S. Boubrit, N. Boussad."Détermination "in vitro" du pouvoir antibactérien des huiles essentielles d'eucalyptus, myrte, clous de girofle et sarriette, et leur application à la conservation de la viande fraîche type hachée". Mémoire du diplôme d’Ingénieur d'Etat en Biologie, option contrôle de la qualité et analyses, Université Mouloud Mammeri de Tizi-ouzou, Algérie, 2007.

F. Chami. "Evaluation in vitro de l’ActionAntifongique des huilesessentiellesd’Origan et de Girofle et de leurs Composés Majoritairesin vivo Application dans la Prophylaxie et le Traitement de la CandidoseVaginalesur des Modèles de Rat et de Souris Immunodéprimés". Thèse de Doctorat, UniversitéSidi Mohamed Ben Abdallah, Maroc, 2005.

L. Jirovetz, G. Buchbauer, I. Stoilova, A. Stoyanova, A. Krastanov, E. Schmidt. "Chemical composition and antioxidant properties of clove leaf essential oil". Journal of Agricultural and Food Chemistry, vol 54, pp. 6303-307, 2006.

G. Razafimamonjison, M. Jahiel, T. Duclos, P. Ramanoelina, F. Fawbush, P. Danthu. "Bud, leaf and stem essential oil composition of Syzygium aromaticum from Madagascar, Indonesia and Zanzibar".International Journal of Basic and Applied Sciences, vol 3, pp. 224-233, 2014.

M. Faucon. Traitéd’aromathérapiescientifique et médicale :fondements& aide à la prescription monographies: huiles essentielles, huiles végétales, hydrolats aromatiques. France. Edition Sang de la Terre,2012, pp. 879.

P. Mailhebiau. La nouvelle aromathérapie: caractérologie des essences ettempéramentshumains. France. Edition Nouvelle Vie, 1989, pp. 372.

J. F. Clevenger. "Apparatus for the determination of volatile oil".Journal of the American Pharmaceutical Association, vol 17, pp. 346-49, 1928.

P. Mbarga, A.P. Nkoa. "Evaluation de la qualité du beurre de cacao vendudansquelquesmarchés de la ville de Yaoundé". Mémoiresoutenuenvue de l’obtention du Diplôme de l’enseignement technique, 1er grade, ENSET de l’Université de Douala, Cameroun, 2014.

R.P. Adams. "Identification of essential oil components by Gas Chromatography / Mass spectrometry". USA. 4th edition, Allured Publishing Corporation, Carol Stream, 2007, pp. 804.

M.E. Cuvelier, H. Richard, C. Berset. "Comparison of the antioxidative Biochem, antioxydative activity of some acid phénols: Structure-activity relationship". Bioscience, Biotechnology, and Biochemistry journal, vol 56, pp. 324-25, 1992.

P. M. Jazet Dongmo, F. Tchoumbougnang, E. Tchinda Sonwa, F. Fekam Boyom, P. H. Amvam Zollo, C. Menut."Antiradical, antioxidant activities and anti-inflammatory potential of the essential oils of the varieties of citrus limon and citrus aurantifolia growing in Cameroon".Journal of Asian Scientific Research, vol 3, pp. 1046-057, 2013.

P. M. JazetDongmo, F. Tchoumbougnang, B. Ndongson , W. Agwanande, B. Sandjon, P. H. AmvamZollo, et al. "Chemical characterization, antiradical, antioxidant and anti-inflammatorypotential of the essential oils of Canariumschweinfurthiiand Aucoumeaklaineana(Burseraceae) growing in Cameroon". Agriculture and biology journal of north America, vol1, pp. 606-11, 2010.

M. Lahlou. "Methods of studying the phytochemistryand bioactivity of Essential Oil". Journal ofPhytotherapy research, vol 18, pp. 435-48, 2004.

R.F. Houinsou, E.S. Adjou, E.D. Ahoussi, D.C.K. Souhouhloué, M. M. Soumanou. "Biochemical and sensorial charactéristics of cowpea (vignaunguiculata)stored with essentials oils extracted from plant of Myrtaceaefamily". International journal of Innovation and Applied Studies, vol 9, pp. 428-37, 2014.

M.L. Sameza, M.A.B. Boat, C.N.M. Lile, S. Tchameni, M. Nguemnang, B.B. Ampere, et al. Evaluation of clove essential oil as a mycobiocide against Rhizopus stolonifer and fusarium solani, tuber

rot causing fungi in yam (discorea rotumdata). Journal of Phytopathology, pp.8, 2016.

G. Razafimamonjison, M. Jahiel, P. Ramanoelina, F. Fawbush, P. Danthu. "Effects of phenological stages on yield and composition of essential oil of Syzygium aromaticum buds from Madagascar". International Journal of Basic and Applied Sciences, vol 2, pp. 312 – 18, 2013.

P. Eke, W.L. Nana, J. Kuate, B.D. Begoude, T. Tchana, C. Menut, et al. "Antifungal potential of Syzygium aromaticumand Zanthoxylum xanthoxyloides essential oils against Phaeoramularia angolensis causing phaeoramularia leaf and fruit Spot disease on citrus". International Journal of Current Microbiology and Applied Sciences, vol 4, pp. 964-76, 2015.

V.K. Raïna, S.K. Srivastava, K.K. Aggarwal, S. Ramesh, S. Kumar. "Essential oil composition of CinnamomumzeylanicumBlume leaves from Little Andaman, India". Flavour and Fragrance Journal, vol 16, pp. 374-76, 2001.

K.A.Srivastava, K.S. Srivastava, V. K. Syamsundar. "Bud and leaf essential oil composition of SyzygiumaromaticumfromIndia and Madagascar". Flavour and Fragrance Journal, vol 20, pp. 51-53, 2005.

E.M. Gaydou, R.P. Randriamiharisoa. "Multidimentional analysis of Gas chromatographic data, application to the differenciation of clove bud and clove stem essential oil from Madagascar". Parfumer and Flavorist, vol12, pp. 45-51, 1987.

S. Ali, R. Prasard, A. Mahmood. "Eugenol-rich Fraction of Syzygium aromaticum(clove) Reverses Biochemical and Histopathological Changes in Liver Cirrhosis and Inhibits Hepatic Cell Proliferation". Journal of Cancer Prevention, vol 19, pp.288-300, 2014.

K. Chaieb, T. Zmantar, R. Ksouri, H. Hajlaoui, K. Mahdouani, C. Abdelly, et al. "Antioxidant properties of the essential oil of Eugenia caryophyllata and its antifungal activity against a large number of clinical Candida

species". Mycoses, vol50, pp. 403-06, 2007.

T. Atsusane. "Clove oil or dehydroeugenol for controlling oxygen in thehuman body".Japan KokaiTokkyoKoho, vol 227, pp. 6, 1991.

S. Barbelet. "Le giroflier : historique, description et utilisations de la plante et de son huile essentielle". Mémoire de thèse soutenu en vue de l’obtention du diplôme d'Etat de Docteur en Pharmacie, Université de Lorraine, France, 2015.

J.P. Dai, X.F. Zhao, J. Zeng, Q.Y.Wan, J.C. Yang, W.Z. Li, et al."Drug screening for autophagy inhibitors based on the dissociation of Beclin1-Bcl2 complex using BiFC technique and mechanism of eugenol on anti-influenza A virus activity". PLoS One, vol 8, pp.1-9, 2013.

H. Nam, M.M. Kim. "Eugenol with antioxidant activity inhibits MMP-9 related to metastasis in human fibrosarcomacells". Food and chemical toxicology, vol55, pp. 106-12, 2013.


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