Microbial Quality of Preserved Sardines Sold in Mombasa
The research was conducted in five different market centres in Mombasa to investigate microbiological quality of sardines preserved using different techniques. A total of thirty (30) sardines were randomly sampled and purchased for analysis of pathogenic and spoilage microorganisms. The microbial load (bacteria and moulds) were determined using pour plate technique. The results showed that out of the six preservation techniques (smoking, drying, salting, freezing, frying and canning) sardines preserved using five of the techniques (83.3%) were contaminated with Vibrio species while two techniques (33.3%) allowed the growth of Salmonella species. Other bacterial species found in preserved sardines included Micrococcus, Staphylococcu and Listeria species among many others. There were also five species of fungi isolated from preserved sardines including Aspergillus species which is of clinical importance. The presence of pathogenic bacteria and fungi of clinical importance are some of the possible health risks that may be associated with consumption of preserved sardines sold in Mombasa. This may possess real health problems unless measures are put in place to prevent microbial contamination of preserved sardines. The total microbial contamination of sardine samples were compared to International Commission on Microbiological Specifications for Foods (ICMSF, 1998) shown in appendix I. Based on total microbial considering bacteria and fungi, smoked sardines had 3.57 x 103, dried 5.096 x 103, salted 2.528 x 103, frozen 3.74 x 102, fried 3.72 x 102 and canned 78 cfu. This showed that dried sardines were the most contaminated while canned were least contaminated.
. Adu – Gyamfi, A. 2006. Studies on microbiological quality of smoked fish in some markets in Accra Ghana. Journal of science 46: 67 – 78.
. Ahmed, E. O., Kalid, M. E., Taha, R. A. and Mahammed, H. M.2010. Investigating the quality changes of hot smoked Oreochromis niloticus and Clarias lazera. Pakistan. Journal of Nutrition, 9(5): 481 – 484.
. Awuor, C. B., Orindi, V. A. and Adwera, A. 2008. Climatic change and coastal cities, the case of Mombasa, Kenya. Environment and Urbanization, 20 (1): 231 – 242.
. Bahri, P., Ayse, G., Gulsum, O. and Irfan, J. 2006. Microbiological and chemical qualities of salted grey mullet. International journal of science technology, 2: 91 – 98.
. Banjo, A. D., Lawal, O. A. and Adeyemi, A. I. 2002. Microorganisms associated with dried fish, Nigeria. Applied microbiology, 68: 4307 – 4314.
. Dalgaard, P. 1993. Evaluation and prediction of microbial fish spoilage. Ph,D – thesis, Technological laboratory, Danish Ministry of Fisheries, Lyngby, Denmark, pp. 169.
. FAO/WHO, 2003. International seafood trade. Journal of economics, vol. 33. No. 1. February, pp. 122 – 132
. Gram, L. and Wedelle – Neergard , P. 1990. The bacteriology of fresh and spoiling lake Victorian Nile perch (Lates niloticus). Journal of food microbiology, vol. 10. pp. 303 – 316.
. Guye, B. and Jemmi. 2007. Clinical Microbiology of Bacterial and Fungal sepsis. Norway. Journal of Public Health, 97 (4): 66 – 675.
. Kris – Etherton, P. M., Harris, W. S., Apee, U. 2003. Fish consumption, fish oil, Omega – 3fatty acids and cardiovascular disease. Circulation 106 (21): 2447 – 2757.
. Mensah, P., Yeboah – menu, D., Owusu – Darko, K. and Ablordey, A. 2002. Street foods in Accra, Ghana. Bulletin of the WHO, 80 (7): 546 – 554.
. Miller, M. L. and Koburger, J. A. 2007. Plesiomonas shigelloids; an opportunistic food and water borne pathogen. Journal of food protection, 48: 449.
. Nketsia, T. 2007. Quality attributes of cured fish in Ghana. Journal of applied science technology 5, (1&2): 148 – 155.
. Obodai, E. A., Nyarko, H. D., Boamposen, L. K., Coomson, S. S. and Aniwe, Y. 2011. Microbial Profile of smoked sardines (Sardinella aurita) at smoking sites and market centres of Tema, Ghana. Archives of applied science research, 3(3): 443 – 453.
. Obuz, E. and Dikeman, M. E. 2003. Effect of smoking and frozen storage on microbiological and nutrient quality of some African fish Ghana. Journal of science, pp. 22 – 29.
. Reilly, P. J., Fuchs, R. S., Barbara, M. and Lund, A. C. 1992. Review of occurrence of Salmonella in tropical shrimp, Rome, Italy. Journal of food science, pp. 315.
. Todar, K. 2009. Bacterial resistance to antibiotics. Malaysia, Nutrition and growth of bacteria, pp. 13.
. Weagant, S. D., Sado, P. A. and Colburn, K. G. 2009. Incidence of Listeria species in tropical fish, India. Tropical products institute, pp. 51 – 60
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